본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Ex Vivo Permeability Characteristics of Porcine Buccal Mucosa to Drugs with Various Polarity

Lee, Jae-Hwi    (College of Pharmacy, Chung-Ang University   ); Lee, Yoon-Jin    (College of Pharmacy, Chung-Ang University   ); Yoon, Mi-Kyeong    (College of Pharmacy, Chung-Ang University   ); Choi, Young-Wook    (College of Pharmacy, Chung-Ang University  );
  • 초록

    The aim of this study was to analyze characteristics of the barrier function of excised porcine buccal mucosa to the test compounds, estradiol, propranolol HCI, melatonin, and mannitol with a wide range of partition coefficient values. The permeability of melatonin was measured through frozen, stored, and fresh porcine buccal mucosa to examine the impact of storage conditions on the permeability of porcine buccal mucosa. The results demonstrated that the ex vivo permeability of the porcine buccal mucosa was greater for more lipophilic solutes, which was consistent with a series of molecules transported by passive transepithelial diffusion. The melatonin permeation profiles through frozen, stored, and fresh mucosa illustrated that damage was incurred by the freezing process of the mucosal tissue, leading to loss of the barrier function and thereby an increased permeation coefficient. It can be observed that the influence of compound lipophilicity on the association of the compounds with buccal mucosa was clear. The relationship between permeation coefficient and Log P values for the four compounds investigated demonstrated a proportional relationship, further confirming the importance of the lipophilicity of a compound to permeate the buccal mucosa. These results showed that the ex vivo porcine buccal mucosa model is a suitable tool to screen oral mucosal permeability.


  • 주제어

    Buccal drug delivery .   Porcine buccal Mucosa .   Drug polarity .   Permeability.  

  • 참고문헌 (16)

    1. M.J. Rathbone, B.K. Drunnnond and I.G. Tucker, The oral cavity as a site for systemic drug delivery, Adv. Drug Del. Rev., 13, 1-22 (1994) 
    2. M.E. De Vries, H.E. Bodde, J.C. Verhoef, and H.E. Junginger, Developments in buccal drug delivery, Crit. Rev. Ther. Drug Carr. Sys., 8, 271-303 (1991) 
    3. C.A. Lesch, C.A. Squier, A. Cruchley, D.M. Williams and P. Speight, The permeability of human oral mucosa and skin to water, J. Dent. Res., 68, 1345-1349 (1989) 
    4. C.A. Squier, The permeability of keratinized and nonkeratinized oral epithelium to horseradish peroxidase, J. Ultrastruct. Res., 43, 160-177 (1973) 
    5. K. Tsutsumi, Y. Obata, K. Takayama, K. Isowa and T. Nagai, Permeation of several drugs through keratinized epithelial-free membrane of hamster cheek pouch, Int. J. Pharm., 177, 7-14 (1999) 
    6. R.M. Eggerth, Z.A. Rashidbaigi, M. Mahjour, F.W. Goodhart and M.B. Fawzi, Evaluation of hamster cheek pouch as a model for buccal absorption. Proceed Int. Symp. Control. Rel. Bioact. Mat., 14, 180-181 (1987) 
    7. J. Lee, S.K. Lee and Y.W. Choi, The effect of storage conditions on the permeability of porcine buccal mucosa, Arch. Pharm. Res., 25, 546-549 (2002)     
    8. A. Shojaei and X. Li, Determination of transport route of acyclovir across buccal mucosa, Proceed. Int. Symp. Control. Rel. Bioact. Mat., 24, 427-428 (1997) 
    9. G. DeGrande, L. Benes, F. Horriere, H. Karsenty, C. Lacoste, R. McQuinn, J.H. Guo and R. Scherrer, Specialized oral mucosal drug delivery systems: Patches: In Oral Mucosal Drug Delivery, M.J. Rathbone (Ed.), Marcel Dekker, New York, U.S.A., pp. 285-317 (1996) 
    10. M. Yazdanian, The effect of freezing on cattle skin permeability, Int. J. Pharm., 103, 93-96 (1994) 
    11. P.P.H. Le Brun, P.L.A. Fox, M.E. de Vries and H.E. Bodde, In vitro penetration of some $\beta$-adrenoreceptor blocking drugs through porcine buccal mucosa, Int. J. Pharm., 49, 141-145 (1989) 
    12. R.E. Kates, Absorption kinetics of sub lingually administered propranolol, J. Med., 8, 393-398 (1977) 
    13. M.E. Dowty, K.E. Knuth, B.K. Irons, and J.R. Robinson, Transport of thyrotropin releasing hormone in rabbit buccal mucosa in vitro, Pharm. Res., 9, 1113-1122 (1992) 
    14. J. Swarbrick, G. Lee and J. Brom, Drug permeation through human skin: I. Effect of storage conditions of skin, J. Invest. Dermatol., 78, 63-66 (1982) 
    15. T. Nagai and R. Konishi, Buccal/gingival drug delivery systems, J. Controlled Rel., 6, 353-360 (1987) 
    16. A.J. Hoogstraate and H.E. Bodde, Method for assessing the buccal mucosa as a route of drug delivery, Adv. Drug Del. Rev., 12, 99-125 (1993) 

 저자의 다른 논문

  • Lee, Jae-Hwi (28)

    1. 2004 "뉴로메드정(옥시라세탐 800 mg)에 대한 뉴라세탐정의 생물학적동등성" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 34 (3): 215~222    
    2. 2004 "친수성 폴리머 제피를 이용한 말레인산암로디핀 정제의 안정성 개선" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 34 (5): 407~411    
    3. 2004 "2 × 2 교차설계에 의한 생물학적동등성시험에서 결측치가 있을 때의 통계적 해석 방법" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 34 (5): 379~383    
    4. 2004 "수성미세채널을 형성하는 서방성 매트릭스 장용정을 이용한 탐스로신의 방출제어" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 34 (6): 471~475    
    5. 2004 "타가메트정 400 mg에 대한 신일시메티딘정 400 mg의 생물학적동등성시험" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 34 (6): 521~527    
    6. 2005 "현탁된 고형지질나노입자 내로 친수성 약물의 봉입률을 증대시키기 위한 w/o/w 에멀션 가온용융유화법의 평가" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 35 (1): 33~38    
    7. 2005 "Buccal Delivery of [D-Ala2, D-Leu5]Enkephalin Incorporated in Mucoadhesive Poly(acrylic acid) Hydrogels" 藥劑學會誌 = Journal of Korean pharmaceutical sciences 35 (5): 369~373    
    8. 2005 "Formulation of Microemulsion Systems for Transdermal Delivery of Aceclofenac" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 28 (9): 1097~1102    
    9. 2006 "Mechanism of Oxygenation of 2,6-Di-tert-butylphenol Derivative" Bulletin of the Korean Chemical Society 27 (1): 33~34    
    10. 2006 "High-Performance Liquid Chromatographic-Tandem Mass Spectrometric Determination of Itraconazole in Human Plasma for Bioavailability and Bioequivalence Studies" Bulletin of the Korean Chemical Society 27 (2): 291~294    
  • Lee, Yoon-Jin (3)

  • 윤미경 (9)

  • Choi, Young-Wook (67)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기