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키토산분해효소의 분류와 효소적 특성
Enzymatic Characterization and Classifications of Chitosanases

정우진   (전남대학교 응용생물공학부,전남대학교 농업과학기술연구소UU0001112  ); 국주희   (전남대학교 응용생물공학부,전남대학교 농업과학기술연구소UU0001112  ); 김길용   (전남대학교 응용생물공학부,전남대학교 농업과학기술연구소UU0001112  ); 박지용   (광주과기원 생명과학과UU0000201  ); 박노동   (전남대학교 응용생물공학부,전남대학교 농업과학기술연구소UU0001112  );
  • 초록

    키토산분해효소(Chitosanases, EC 3.2.1.132)는 당질가수분해효소군의 하나로, 아미노당인 D-glucosamine polymer인 chitosan의 ${\beta}-1,4-glycoside$ 결합을 가수분해하는 효소이며, 세균, 곰팡이, 식물 등에 널리 분포한다. 본 논문에서는 chitosanase의 N-말단 아미노산의 서열과 입체구조에 근거한 family 및 clan 분류, 작용모형, 절단 유형, subclass 분류, 및 family-subclass 상관성을 검토하였다. 아미노산 서열과 입체구조와 기질의 분해패턴 사이에는 깊은 상관이 있음을 확인 제시하였다. 다양한 종 유래 chitosanase의 1차구조의 해명과 진화적 상관 규명, 나아가 보다 정교한 chitosanase의 정의와 다양한 산업적 응용에의 가능성도 검토하였다.


    Many chitosanases, glycosyl hydrolases that catalyze the degradation of chitosan, have been found in microorganism. In this paper, classification of the enzyme has been described, which is based on the amino acid sequence (families) and splitting patterns (subclasses). Glycohydrolytic mechanisms such as inversion and retention of the substrate anomer are also discussed in context of the families. Interrelationship among the primary structure, clan, anomeric conversion and the splitting patterns has been suggested. In addition, advanced definition of chitosanase was introduced through the investigation of enzymatic products from partially N-acetylated chitosan as a substrate.


  • 주제어

    키토산분해효소 .   당류 가수분해효소 .   작용기작 .   당전이반응 .   미생물 키토산분해효소 분류 .   키토산분해효소 계통.  

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