본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biology = 식물학회지 v.48 no.2, 2005년, pp.237 - 244   피인용횟수: 2

A Double Mutant for the CYP85A1 and CYP85A2 Genes of Arabidopsis Exhibits a Brassinosteroid Dwarf Phenotype

Kwon Mi    (Department of Biological Sciences, College of Natural Sciences, Seoul National University   ); Fujioka Shozo    (RIKEN(Institute of Physical and Chemical Research)   ); Jeon Ji Hyun    (Department of Biological Sciences, College of Natural Sciences, Seoul National University   ); Kim Ho Bang    (Department of Biological Sciences, College of Natural Sciences, Seoul National University   ); Takatsuto Suguru    (Department of Chemistry, Joetsu University of Education   ); Yoshida shigeo    (RIKEN(Institute of Physical and Chemical Research)   ); An Chung Sun    (Department of Biological Sciences, College of Natural Sciences, Seoul National University   ); Choe Sunghwa    (Department of Biological Sciences, College of Natural Sciences, Seoul National University  );
  • 초록

    Brassinosteroid (BR)-6-oxidases mediate the bridge reactions that connect the late and early C-6 oxidation pathways by converting 6-deoxoBR to 6-oxoBRs. Two similar genes of Arabidopsis, CYP85A1 (At5g38970) and CYP85A2 (At3g30180), are proposed to encode BR-6-oxidases based on findings that heterologously expressed genes mediate BR-6-oxidation reactions in yeast. However, genetic evidence that both genes are critically involved in the BR-6-oxidation step in Arabidopsis has been limited. Here, we show that a double mutant for the two genes displays dwarfism similar to that of typical BR biosynthesis-deficient mutants, suggesting that they are the major BR-6-oxidases in Arabidopsis. Examination of endogenous BR levels and metabolism monitoring tests using this double mutant revealed a great reduction in the levels of 6-oxoBRs, e.g., TY and CS, due to a lack in the conversion reactions from 6-deoxoCS to CS, and from 6-deoxoTY to TY. Surprisingly, the double mutant accumulated a significant amount of 6-oxocampestanol, suggesting that the upstream C-6 oxidation of campestanol to 6-oxocampestanol is not catalyzed by the two BR-6-oxidases in Arabidopsis, rather, by another enzyme yet to be discovered.


  • 주제어

    brassinosteroid .   BR-6-oxidase .   C-6 oxidation .   cell elongation .   CYP85 .   cytochrome P450.  

  • 참고문헌 (19)

    1. Asami T, Mizutani M, Fujioka S, Goda H, Min YK, Shimada Y, Nakano T, Takatsuto S, Matsuyama T, Nagata N, Sakata K, Yoshida S (2001) Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta. J Biol Chem 276: 25687-25691 
    2. Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA (2001) Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J 26: 573-582 
    3. Kwon M, Lee HK, Choe S (2005) Novel simple sequence length polymorphic (SSLP) markers for positional cloning in Arabidopsis thaliana. Kor J Genet 27: 1-8 
    4. Kang JG, Yun J, Kim DH, Chung KS, Fujioka S, Kim JI, Dae HW, Yoshida S, Takatsuto S, Song PS, Park CM (2001) Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth. Cell 105: 625-636 
    5. Shimada Y, Coda H, Nakamura A, Takatsuto S, Fujioka S, Yoshida S (2003) Organ-specific expression of brass inosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis. Plant Physiol 131: 287-297 
    6. Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S (2005) The last reaction producing brassinolide is catalyzed by cytochrome P450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis. J Biol Chem 280: 17873-17879 
    7. Fujioka S, Noguchi T, Watanabe T, Takatsuto S, Yoshida S (2000) Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus. Phytochem 53: 549-553 
    8. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657 
    9. Kwon M, Choe S (2005) Brassinosteroid biosynthesis and dwarf mutant. J Plant Biol 48: 1-15     
    10. Hong Z, Ueguchi-Tanaka M, Shimizu-Sato S, Inukai Y, Fujioka S, Shimada Y, Takatsuto S, Agetsuma M, Yoshida S, Watanabe Y, Uozu S, Kitano H, Ashikari M, Matsuoka M (2002) Loss-of-function of a rice brassinosteroid, biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J 32: 495-508 
    11. Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamlya Y, Bishop GJ, Yoshida S (2001) Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis. Plant Physiol 126: 770-779 
    12. Nomura T, Sato T, Bishop GJ, Kamiya Y, Takatsuto S, Yokota T (2001) Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis. Phytochem 57: 171-178 
    13. Hong,Z, Ueguchi-Ianaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M (2003) A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell 15: 2900-2910 
    14. Choe S (2005) Brassinosteroid biosynthesis and metabolism. In Davies PJ, ed, Plant Hormones: Biosynthesis, Signal Transduction, and Action. Springer, Syracus, pp 156-178 
    15. Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier C, Gregory B, Ross A, Tanaka A, Yoshida S, Tax F, Feldmann KA (1999) The Arabidopsis dwf7/ste1 mutant is defective in the delta 7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Plant Cell 11 : 207-221 
    16. Noguchi T, Fujioka S, Choe S, Takatsuto S, Yoshida S, Yuan H, Feldmann KA, Tax FE (1999) Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol 121: 743-752 
    17. Bishop GJ, Harrison K, Jones JD (1996) The tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family. Plant Cell 8: 959-969 
    18. Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto S, Jones JD,.Kamiya Y (1999) The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci USA 96: 1761-1766 
    19. Noguchi T, Fujioka S, Choe S, Takatsuto S, Tax FE, Yoshida S, Feldmann KA (2000) Biosynthetic pathways of brassinolide in Arabidopsis. Plant Physiol 124: 201-209 
  • 이 논문을 인용한 문헌 (2)

    1. 2007. "" Journal of plant biology = 식물학회지, 50(3): 225~229     
    2. 2011. "" Journal of plant biology = 식물학회지, 54(1): 10~14     

 저자의 다른 논문

  • 권미 (4)

    1. 1991 "낙우송(落羽松)(Taxodium distichum Rich.) 지재(枝材)의 압축이상재(壓縮理想材) 및 대응재(對應材)에 관한 해부학적(解剖學的) 특성(特性)" 韓國林學會誌 = Journal of Korean Forest Society 80 (3): 296~302    
    2. 1993 "갈색부후균(Lentinus lepideus)에 의해 부후된 소나무 재(Pinus densiflora S. et Z.)의 해부학적 특성" 목재공학 = Journal of the Korean wood science and technology 21 (1): 39~50    
    3. 2005 "Brassinosteroid Biosynthesis and dwarf Mutants" Journal of plant biology = 식물학회지 48 (1): 1~15    
    4. 2006 "An Arabidopsis short root and dwarfism Mutant Defines a Novel Locus That Mediates Both Cell Division and Elongation" Journal of plant biology = 식물학회지 49 (1): 61~69    
  • Jeon, Ji-Hyun (1)

  • Kim, Ho-Bang (15)

  • An, Chung-Sun (42)

  • Choe, Sung-Hwa (8)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기