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Journal of plant biology = 식물학회지 v.48 no.2, 2005년, pp.253 - 257   피인용횟수: 1

Micropropagation of Dendrobium fimbriatum Hook. by Green Pod Culture

Sharma Ramnath    (Post Graduate Department of Botany, Darjeeling Government College   ); De K.K.    (Post Graduate Department of Botany, Darjeeling Government College   ); Sharma Bibaychana    (Post Graduate Department of Botany, Darjeeling Government College   ); Majumdar Sudipta    (Post Graduate Department of Botany, Darjeeling Government College  );
  • 초록

    Present studies have demonstrated that immature seeds obtained from green pod of Dendrobium fimbriatum Hook., an endangered epiphytic forest orchid having horticultural importance, can be germinated asymbiotically in vitro for rapid micropropagation. Vacin and Went medium containing $0.1mg\;L^{-1}\;NAA\;and\;15\%$ coconut water was found most effective for high percentage $(80-90\%)$ seed germination and seedling development. This method can be exploited for their rapid micropropagation and conservation.


  • 주제어

    Dendrobium fimbriatum .   green pod culture .   micropropagation.  

  • 참고문헌 (14)

    1. Knudson I (1951) Nutrient Solutions for Orchids. Bot Gaz 112: 528-532 
    2. Mcintyre KK, Vietch GJ, Wrigley JW (1974) Australian terrestrial orchids from seeds II. Improvement in technique and further success. Am Orchid Soc Bull 43: 52-53 
    3. Monaco LC, Sondahl MR, Carvalho A, Crocomo OJ, Sharp WR (1977) Applications of tissue culture . in the improvement of coffee, In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Springer-Verlag, Berlin, Heidelberg, pp 109-129 
    4. Nimoto DH, Sagawa (1961) Ovule development in Dendrobium. Am Orchid Soc Bull 30: 813-819 
    5. Intuwong C, Sagawa Y (1975) Clonal propagation of Dendrobium 'Golden Wave' and other nobile types. Am Orchid Soc Bull 44: 319 
    6. Kotomori S, Murashige T (1965) Some aspects of aseptic . propagation of orchids. Am Orchid Soc Bull 34: 484-489 
    7. Lawrence D, Arditti J (1964) A new medium for the germination of orchid seed. Am Orchid Soc Bull 33: 766-768 
    8. Sharma RN, Sharma B, De KK (2004) Micropropagation of Dendrobium fimbriatum Hook. - An endangered orchid of Sikkim Himalaya. J Hill Res 17: 30-35 
    9. Salenda RP (1976) Harvesting time of orchid seed capsule for the green pod culture process. Am Orchid Soc Bull 45: 305-309 
    10. Vacin E, Went F (1949) Some pH changes in nutrient solutions. Bot Gaz 110: 605-613 
    11. Sarma CM (2002) Micropropagation of Dendrobium fimbriatum var occulatum from seeds. J Adv Plant Sci 2: 22-25 
    12. Kumaria S, Tandon P (2000) Effect of growth regulators on peroxidase, polyphenol oxidase and 1M oxidase activities and phenol contents during protocorm development of Dendrobium fimbriatum var. oculatum H.k.F. J Orchid Soc India 14: 27-39 
    13. George EF (1996) The trade term for aseptic seed germination is flasking, Plant Propagation by Tissue Culture, Ed 2, Vol 2. Exegetics Limited, London, pp 921 
    14. Matthews VH, Rao PS (1980) In vitro multiplication of Vanda hybrids through tissue culture technique. Plant Sci Lett 17: 383-389 
  • 이 논문을 인용한 문헌 (1)

    1. 2006. "" Journal of plant biology = 식물학회지, 49(2): 167~173     

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