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Genetic Transformation of Ajuga multiflora Bunge with Agrobacterium rhizogenes and 20-Hydroxyecdysone Production in Hairy Roots

Kim Ok Tae    (Department of Biology and Institute of Plant Resources, Chonnam National University   ); Manickavasagm M.    (Department of Biotechnology, Bharathidasan University   ); Kim Young Jun    (Department of Biology and Institute of Plant Resources, Chonnam National University   ); Jin Mei Ran    (Department of Biology and Institute of Plant Resources, Chonnam National University   ); Kim Kwang Soo    (Mokpo Experiment Station, National Institute of Crop Science, RDA   ); Seong Nak Sul    (National Institute of Crop Science, RDA   ); Hwang Baik    (Department of Biology and Institute of Plant Resources, Chonnam National University  );
  • 초록

    An efficient transformation system for Ajuga multiflora Bunge was established by using Agrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformed Ajuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type.


  • 주제어

    Ajuga multiflora .   hairy roots .   20-hydroxyecdysone .   Ri-plasmid .   rol genes.  

  • 참고문헌 (19)

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