본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

Kim, Mu-Chan    (Department of Marine Environmental Engineering, Gyeongsang National University   ); Park, Chan-Il    (Department of Marine Biology & Aquaculture, Gyeongsang National University  );
  • 초록

    Two distinct CD3 homologue genes, $CD3\gamma/\delta\;and\;CD\varepsilon$ , were isolated from a olive flounder leukocyte cDNA library and a BAC library. $CD3\gamma/\delta$ consisted of 961 bp encoding 178 amino acid residues, and $CD3\varepsilon$ consisted of 1006 bp encoding 164 amino acid residues. When compared with other known CD3 peptide sequences, the most conserved region of the two olive flounder CD3 chain peptides are the cytoplasmic domain and the least conserved are the extracellular domain. A phylogenetic analysis based on the deduced amino acid sequence grouped the two olive flounder CD3 sequences with $CD3\varepsilon$ and $CD3\gamma/\delta$ , respectively. The olive flounder CD3 cluster (consisting of $CD3\varepsilon\;and\;CD3\gamma/\delta$ ) spans only 10.4 kb. The $CD3\varepsilon\;and\;CD3\gamma/\delta$ genes are oppositely transcribed only 3.8 kb apart. Both olive flounder CD3 genes have five exons. The two olive flounder CD3 genes were predominantly expressed in PBLs, kidney, spleen, and gills.


  • 주제어

    Olive flounder .   CD3 .   ITAM .   T cell receptor .   Teleost.  

  • 참고문헌 (29)

    1. Bernot, A. and C. Auffray. 1991. Primary structure and ontogeny of an avian CD3 transcript. Proc. Natl. Acad. Sci. USA, 88, 2550-2554 
    2. Bertram, E.M., R.G. Wilkinson, B.A. Lee, A.R. Jilbert and I. Kotlarski. 1996. Identification of duck T lymphocytes using an anti-human T cell (CD3) antiserum. Vet. Immunol. Immunopathol., 51, 353-363 
    3. Boris, Y.A., V.G. Sergei, M.N. Alexander, Y.M. Ludmila and Y.T. Alexander. 2000. CD3$\varepsilon$ homologues in the chondrostean fish Acipenser ruthenus. Immunogenetics, 51, 1012-1020 
    4. Boyer, C., N. Auphan, F. Luton, J.M. Malburet, M. Barad, J.P. Bizozzero, H. Reggio and A.M. Schmitt-Verhulst. 1991. T cell receptor/CD3 complex internalization following activation of a cytolytic T cell clone: evidence for a protein kinase C-independent staurosporine-sensitive step. Eur. J. Immunol., 21, 1623-1634 
    5. Buferne, M., F. Luton, F. Letourneur, A. Hoeveler, D. Couez, M. Barad, B. Malissen, A.M. Schmitt-Verhulst and C. Boyer. 1992. Role of CD3 delta in surface expression of the TCR/CD3 complex and in activation for killing analyzed with a CD3 delta-negative cytotoxic T lymphocyte variant. J. Immunol., 148, 657-664 
    6. Clevers, H.C., S. Dunlap, T.E. Wileman and C. Terhorst. 1988b. Human CD3-$\varepsilon$gene contains miniexons and is transcribed from a non-TATA promoter. Proc. Natl. Acad. Sci. USA, 85, 8156-8160 
    7. Dzialo, R.C. and M.D. Cooper. 1997. An amphibian CD3 homologue of the mammalian CD3 gamma and delta genes. Eur. J. Immunol., 27, 1640-1647 
    8. Geisler, C. 1992. Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. J. Immunol., 148, 2437-2445 
    9. Gobel, T.W. and M. Fluri. 1997. Identification and analysis of the chicken CD3 $\varepsilon$ gene. Eur. J. Immunol., 27, 194-198 
    10. Hirono, I., B.H. Nam, T. Kurobe and T. Aoki. 2000. Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralichthys olivaceus. J. Immunol., 165, 4423 -4427 
    11. Huppa, J.B. and H.L. Ploegh. 1997. In vitro translation and assembly of a complete T cell receptor-CD3 complex. J. Exp. Med., 186, 393-403 
    12. Katagiri, T., S. Asakawa, I. Hirono, T. Aoki and N. Shimizu. 2000. Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus. Mar. Biotechnol., 2, 571-576 
    13. Klausner, R.D., J. Lippincott-Schwartz and J.S. Bonifacino. 1990. The T cell antigen receptor: insights into organelle biology. Ann. Rev. Cell Biol., 6, 403-431 
    14. Krangel, M.S. 1987. Endocytosis and recycling of the T3- T cell receptor complex. The role of T3 phosphorylation. J. Exp. Med., 165, 1141-1159 
    15. Krangel, M.S., B.E. Bierer, P. Devlin, M. Clabby, J.L. Strominger, J, McLean and Brenner M.B. 1987. T3 glycoprotein is functional although structurally distinct on human T-cell receptor gamma T lymphocytes. Proc. Natl. Acad. Sci. USA, 84, 3817-3821 
    16. Krissansen, G.W., M.J. Owen, P.J. Fink and M.J. Crumpton. 1987. Molecular cloning of the cDNA encoding the T3 gamma subunit of the mouse T3/T cell antigen receptor complex. J. Immunol., 138, 3513-3518 
    17. Mallabiabarrena, A., M. Fresno and B. Alarcon. 1992. An endoplasmic reticulum retention signal in the CD3$\varepsilon$ chain of the T-cell receptor. Nature, 357, 593-596 
    18. Manolios, N., F. Letourneur, J.S. Bonifacino and R.D. Klausner. 1991. Pairwise, cooperative and inhibitory interactions describe the assembly and probable structure of the T-cell antigen receptor. EMBO J., 10, 1643-1651 
    19. Nam, B.H., I. Hirono and T. Aoki. 2003. Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A/PMA. Fish Shellfish Immunol., 14, 467-476 
    20. Niedergang, F., A. Dautry-Varsat and A. Alcover. 1997. Peptide antigen or superantigen-induced downergulation of TCRs involves both stimulated and unstimulated receptors. J. Immunol., 159, 1703-1710 
    21. Pasquier, L., M. Courtet and J. Robert. 1995. A Xenopus lymphoid tumor cell line with complete Ig genes rearrangements and T-cell characteristics. Mol. Immunol., 32, 583-593 
    22. Reth, M. 1989. Antigen receptor tail clue. Nature, 338, 383-344 
    23. Rubin, B.J. Arnaud, S. Caspar-Bauguil, F. Conte and Huchenq 1994. A. Biological function of the extracellular domain of the T-cell receptor constant region. Scand. J. Immunol., 39, 517-525 
    24. Sussman, J.J., J.S. Bonifacino, J. Lippincott-Schwartz, A.M. Weissman, T. Saito, R.D. Klausner and J.D. Ashwell. 1988. Failure to synthesize the T cell CD3- zeta chain: structure and function of a partial T cell receptor complex. Cell, 52, 85-95 
    25. Wange, R.L. and L.E. Samelson. 1996. Complex complexes: signaling at the TCR. Immunity, 5, 197-205 
    26. Charlemagne, J., J.S. Fellah, A. De Guerra, F. Kerfourn and S. Partula. 1998. T-cell receptors in ectother-mic vertebrates. Immunol. Rev., 166, 87-102 
    27. Gobel, T.W. and J.P. Dangy. 2000. Evidence for a stepwise evolution of the CD3 family. J. Immunol., 164, 879-883 
    28. Park C.I., I. Hirono, J. Enomoto, Nam B.H., T. Aoki. 2001. Cloning of Japanese flounder Paralichthys olivaceus CD3 cDNA and gene, and analysis of its expression. Immunogenetics, 53, 130-135 
    29. Clevers, H., B. Alarcon, T Wileman and C. Terhorst. 1988a. The T cell receptor/CD3 complex: a dyna-mic protein ensemble. Annu. Rev. Immunol., 6, 629-662 

 저자의 다른 논문

  • 김무찬 (15)

    1. 2004 "기후변화 적응방안 연구 -해수면 상승을 중심으로-" 海洋環境安全學會誌 = Journal of the Korean society of marine environment & safety 10 (2): 81~88    
    2. 2006 "Cloning and Characterization of DAP10 homologue gene from Olive Flounder, Paralichthys olivaceus" 韓國魚病學會誌= Journal of fish pathology 19 (3): 227~233    
    3. 2006 "Heterosigma akashiwo(Raphidophyceae)을 이용한 북만의 조류성장잠재력 시험" 海洋環境安全學會誌 = Journal of the Korean society of marine environment & safety 12 (2): 81~87    
    4. 2006 "해산어 양식환경하의 미생물군집에 대한 옥소린산의 영향" 海洋環境安全學會誌 = Journal of the Korean society of marine environment & safety 12 (2): 89~98    
    5. 2006 "해양환경에서 부유물질, 염분 및 pH의 옥소린산 화학적 거동에 미치는 영향" 海洋環境安全學會誌 = Journal of the Korean society of marine environment & safety 12 (2): 99~106    
    6. 2006 "물벼룩(Moina macrocopa)배양을 위한 해양효모의 유효성에 대한 안전 동위원소의 증거" Korean journal of microbiology = 미생물학회지 42 (2): 111~115    
    7. 2007 "Cloning and expression of cDNA for chemokine receptor 9 from Olive flounder, Paralichthys olivaceus" 韓國魚病學會誌= Journal of fish pathology 20 (3): 299~306    
    8. 2008 "Temporal Variation in the Chlorophyll α Concentration of the Coastal Waters of Spain Following the Ship Prestige Oil Spill" Journal of fisheries science and technology 11 (4): 212~218    
    9. 2008 "Haemocytes responses of the pearl oyster, Pincdata fucata, at different temperatures" 韓國魚病學會誌= Journal of fish pathology 21 (3): 241~246    
    10. 2008 "참굴(Crassostrea gigas) 수정란에 미치는 나노입자의 영향" 海洋環境安全學會誌 = Journal of the Korean society of marine environment & safety 14 (1): 9~14    
  • Park, Chan-Il (20)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기