본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of fisheries science and technology v.8 no.2, 2005년, pp.109 - 112   피인용횟수: 2

Peptide Inhibitor for Angiotensin-Converting Enzyme from Thermolysin Hydrolysate of Manila Clam Proteins

Lee Tae-Gee    (Department of Hotel Culinary & Baking, Namdo Provincial College of Jeonnam   ); Yeum Dong-Min    (Department of Food Processing & Baking, Yangsan College   ); Kim Young-Sook    (Department of Food Processing & Baking, Yangsan College   ); Yeo Saeng-Gyu    (Department of Hotel Culinary Arts, Busan College of Information Technology   ); Lee Yong-Woo    (Division of Food Science, Dongeui Institute of Technology   ); Kim Jin-Soo    (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University   ); Kim In-Soo    (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University   ); Kim Seon-Bong    (Faculty of Food and Biotechnology, Pukyong National University  );
  • 초록

    A peptide that inhibits angiotensin-converting enzyme (ACE) was isolated from a hydrolysate of Manila clam (Ruditapes philippinarum) proteins prepared with thermolysin. Amino acid sequence of the peptide was determined to be Leu-Leu-Pro. Chemically synthesized Leu-Leu-Pro had an $IC_{50}\;value\;of\;158\;\mu{M}$ . Peptides related to the Manila clam-derived peptide were synthesized to study the structure-activity relationships. The tetrapeptide, Leu-Leu-Pro-Pro, had a very weak effect on the enzyme. However, Leu-Leu-Pro-Asn showed no inhibitory activity.


  • 주제어

    Angiotensin-converting enzyme (ACE) .   Manila clam .   thermolysin hydrolysate .   ACE- inhibitory peptide.  

  • 참고문헌 (15)

    1. Brewer, H.B., Jr., T. Fairwell, A. LaRue, R. Ronan, A. Houser and T.J. Bronzert. 1978. The amino acid sequence of human ApoA-I, an apolipoprotein isolated from high density lipoproteins. Biochem. Biophys. Res. Commun., 80, 623-630 
    2. EI-Dorry, H.A., H.G. Bull, K. Iwata, N.A. Thornberry, E.H. Cordes and R.L. Soffer. 1982. Molecular and catalytic properties of rabbit testicular dipeptidyl carboxypeptidase. J. Biol. Chem., 257, 14128-14133 
    3. Ferreira, S.H., D.C. Bartelt and L.J. Greene. 1970. Isolation of bradykinin-potentiating peptides from Bothrops jararaca venom. Biochemistry, 9, 2583-2593 
    4. Kinoshita, E., J. Yamakoshi and M. Kikuchi. 1993. Purification and identification of an angiotensin I-converting enzyme inhibitor from soy sauce. Biosci. Biotech. Biochem., 57, 1107-1110 
    5. Lee, T.G., Y.B. Park, D.C. Park, D.M. Yeum, I.S. Kim, Y.S. Gu, Y.H. Park and S.B. Kim. 1998. Angiotensin converting enzyme inhibitory activity in enzymatic hydrolysates of anchovy muscle protein. J. Kor. Fish. Soc., 31, 875-881 
    6. Maruyama, S., K. Nakagomi, N. Tomizuka and H. Suzuki. 1985. Angiotensin-converting enzyme inhibitor derived from an enzymatic hydrolysate of casein. II. Isolation and bradykinin-potentiating activity on the uterus and the ileum of rats. Agric. Biol. Chem., 49, 1405-1409 
    7. Matsui, T., H. Matsufuji, E. Seki, K. Osajima, M. Nakashima and Y. Osajima. 1993. Inhibition of angiotensin I-converting enzyme by Bacillus lichen iformis alkaline protease hydrolysates derived from sardine muscle. Biosci. Biotech. Biochem., 57, 922-925 
    8. Matsumura, N., M. Fujii, Y. Takeda, K. Sugita and T. Shimizu. 1993. Angiotensin I-converting enzyme inhibitory peptides derived from bonito bowels autolysate. Biosci. Biotech. Biochem., 57, 695-697 
    9. Miyoshi, S., H. Ishikawa, T. Kaneko, F. Fukui, H. Tanaka and S. Maruyama. 1991. Structures and activity of angiotensin-converting enzyme inhibitors in an $\alpha$-zein hydrolysate. Agric. Biol. Chem., 55, 1313-1318 
    10. Oshima, G., H. Shimabukuro and K. Nagasawa. 1979. Peptide inhibitors of angiotensin l-converting enzyme in digests of gelatin by bacterial collagenase. Biochim. Biophys. Acta, 566, 128-137 
    11. Titani, K., A. Koide, J. Hermann, L.H. Ericsson, S. Kumar, R.D. Wade, K.A. Walsh, H. Neurath and E.H. Fischer. 1977. Complete amino acid sequence of rabbit muscle glycogen phosphorylase. Proc. Natl. Acad. Sci. USA, 74, 4762-4766 
    12. Yang, H.Y.T., E.G. Erdos and Y.A. Levin. 1970. Dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin. Biochim. Biophys. Acta, 214, 374-376 
    13. Ondetti, M.A., N.J. Williams, E.F. Sabo, J. Pluscec, E.R. Weaver and O. Kocy. 1971. Angiotensin-converting enzyme inhibitors from the venom of Bothrops jararaca. Isolation, elucidation of structure, and synthesis. Biochemistry, 10, 4033-4039 
    14. Skeggs, L.T., W.H. Marsh, J.R. Kahn and N.P. Shumway. 1954. The existence of two forms of hypertensin. J. Exp. Med., 99, 275-282 
    15. Kohama, Y., S. Matsumoto, H. Oka, T. Teramoto, M. Okabe and T. Mimura. 1988. Isolation of angiotensinconverting enzyme inhibitor from tuna muscle. Biochem. Biophys. Res. Commun., 155, 332-337 
  • 이 논문을 인용한 문헌 (2)

    1. 2008. "" Journal of food science and nutrition, 13(3): 196~203     
    2. Park, Yeung-Beom 2010. "Characteristics of Angiotensin Converting Enzyme Inhibitory Peptides from Salt-fermented Squid Liver Sauce" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(11): 1654~1659     

 저자의 다른 논문

  • 염동민 (12)

    1. 2000 "냉풍건조 멸치의 식품성분 특성" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 29 (6): 973~980    
    2. 2001 "식품소재로서 굴통조림 가공부산액의 성분 특성" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 30 (2): 299~306    
    3. 2002 "바지락 단백질 Thermolysin 가수분해물의 Angiotensin Converting Enzyme 저해 Peptide의 특성" 한국수산학회지 = Journal of the Korean Fisheries Society 35 (5): 529~533    
    4. 2003 "Isolation of HIV-1 Pretense Inhibiting Peptide from Thermolysin Hydrolysate of Manila Clam Proteins" Nutraceuticals and food 8 (2): 154~157    
    5. 2006 "가다랑어 및 황다랑어 알의 식품성분 특성" 한국수산학회지 = Journal of the Korean Fisheries Society 39 (1): 1~8    
    6. 2007 "새우 부산물을 이용한 기능성 소스의 제조" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 36 (2): 209~215    
    7. 2008 "약용식물을 첨가한 어간장의 품질특성" 한국식품저장유통학회지 = Korean journal of food preservation 15 (3): 367~376    
    8. 2009 "레토르트 열처리 조건에 따른 굴자숙수 농축물의 식품성분 특성 비교" 한국수산과학회지 = Korean journal of fisheries and aquatic sciences 42 (3): 197~203    
    9. 2011 "국산 및 중국산 율무의 이화학적 특성" 식품산업과 영양 = Food industry and nutrition 16 (2): 49~53    
    10. 2012 "알팔파 추출물을 첨가한 두부의 품질 특성" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 41 (1): 123~128    
  • 김진수 (158)

  • 김인수 (43)

  • Kim, Seon-Bong (49)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기