본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Plasma Membrane Transporters for Lead and Cadmium

Bressler, Joseph P.    (Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Kennedy-Krieger Institute   ); Olivi, Luisa    (Kennedy-Krieger Institute   ); Kim, Yong-Bae    (Department of Preventive Medicine, Soonchunhyang University   ); Bannon, Desmond    (US Army, Aberdeen Proving Ground   ); Ko, Hong-Sook    (Department of Pharmacy, Sahmyook University   ); Cheong, Jae-Hoon    (Department of Pharmacy, Sahmyook University  );
  • 초록

    Lead and cadmium are potent environmental toxicants that affect populations living in Europe. Americas, and Asia. Identifying transporters for lead and cadmium could potentially 1 help us better understand possible risk factors. The iron transporter, divalent metal transporter 1 (DMT1), mediates intestinal transport of cadmium, and lead in yeast and fobroblasts overexpressing DMT1. In human intestinal cells knocking down expression of DMT1 attenuated uptake of cadmium and iron but not lead. A possible explanation is the expression of a second transporter for lead in intestine. In astrocytes, however, DMT1 appears to transport lead in an extracellular buffer at pH value. At neutral pH, transport was not mediated by DMT1 but rather by a transporter that is stimulated by bicarbonate and inhibited by 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonic acid. The identity of this lead transporter will beverified by future study.


  • 주제어

    divalent metal transporter 1 (DMT1) .   cadmium .   lead.  

  • 참고문헌 (57)

    1. Alper, S.L. (1998) The band 3-related AE anion exchange gene family. Cell Physiol. Biochem. 4, 265-281 
    2. Alper, S.L., et al. (2002) The AE gene family of Cl/HCO3-exchangers. J. Nephrol. 15 Suppl 5, S41-53 
    3. Bannon, D.I., Abounader, R., Lees, P.S.J. and Bressler, J.P. (2003b) Effect of DMTl Knockdown on iron, cadmium and lead uptake in Caco-2 cells. Am. J. Psysiol. 28, C44-50 
    4. Bannon, D.I., et al. (2002) Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells. Biochem. Biophys. Res. Commun. 295(4), 978-984 
    5. Bannon, D.I., et al. (2003a) Effect of DMTl knockdown on iron, cadmium, and lead uptake in Caco-2 cells. Am. J. Physiol. Cell. Physiol. 284(1), C44-50 
    6. Bellinger, D. (1994) Low-level lead exposure and childrens cognitive function in the preschool years. Peds. 93(2), A28 
    7. Berglund, M., et al. (1994) Intestinal absorption of dietary cadmium in women depends on body iron stores and fiber intake. Environ. Health Perspect. 102(12), 1058-1066 
    8. Bevensee, M.O., Weed, R.A. and Boron, W.F. (1997b) Intracellular pH regulation in cultured astrocytes from rat hippocampus. I. Role Of HCO3. J. Gen. Physiol. 110(4), 453-465 
    9. Canfield, R.L., et al. (2003) Intellectual impairment in children with blood lead concentrations below 10 microg per deciliter. N. Engl. J. Med. 348(16), 1517-1526 
    10. Cerklewski, F.L. and Forbes, R.M. (1976) Influence of dietary zinc on lead toxicity in the rat. J. Nutr. 106(5), 689-696 
    11. Cheng, Y, et al. (2001) Bone lead and blood lead levels in relation to baseline blood pressure and the prospective development of hypertension: the Normative Aging Study. Am. J. Epidemiol. 153(2), 164-171 
    12. Cheong, J.H., Bannon D.I., Olivi L., Kim, Y.B. and Bressler, J.P. (2004) Different Mechanisms Mediate Uptake of Lead in a Rat Astroglial Cell Line. Toxicol. Sci. 77, 334-340 
    13. Cobbett, C.S. (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol. 123(3), 825-832 
    14. de Kloet, E.R. and M.S. Oitzl (2003) Who cares for a stressed brain? The mother, the kid or both? Neurobiol. Aging 24 Suppl 1, S61-65; discussion S67-68 
    15. Donovan, A., et al. (2000) Positional cloning of zebrafish ferroportinl identifies a conserved vertebrate iron exporter [see comments]. Nature 403(6771), 776-781 
    16. Drasch, G.A., et al. (1983) An increase of cadmium body burden for this century--an investigation on human tissues. Sci. Total Environ. 26(2), 111-119 
    17. Eide, D.J. (2004) The SLC39 family of metal ion transporters. Pflugers Arch. 447(5), 796-800 
    18. el-Waseef, A. and Hashim, M.M. (1985) Zinc lead-interaction in the rabbit. Acta. Med. Hung. 42(3-4), 199-207 
    19. Flanagan, P.R., et al. (1978) Increased dietary cadmium absorption in mice and human subjects with iron deficiency. Gastroenterology. 74(5 Pt 1), 841-846 
    20. Fleming, M.D., et al. (1998) Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport. Proc. Natl. Acad. Sci. 95(3), 1148-1153 
    21. Gaither, L.A. and Eide, D.J. (2000) Functional expression of the human hZIP2 zinc transporter. J. Biol. Chem. 275(8), 5560-5564 
    22. Guerinot, M.L. (2000) The ZIP family of metal transporters. Biochim. Biophys. Acta. 1465(1-2), 190-198 
    23. Gunshin, H., et al. (1997) Cloning and characterization of a mam-malian proton-coupled metal-ion transporter. Nature 388(6641), 482-488 
    24. Hediger, M.A., et al. (2004) The ABCs of solute carriers: physiological, pathological and therapeutic implications of human membrane transport proteinsIntroduction. Pflugers Arch. 447(5), 465-468 
    25. Hertz-Picciotto, I. and Croft, J. (1993) Review of the relation between blood lead and blood pressure. Epidemiol. Rev. 15(2), 352-373 
    26. Holtzman, D., et al.(1984) Maturation of resistance to lead encephalopathy: Cellular and subcellular mechanisms. Neurotoxicology 5(3), 97-124 
    27. Lal, B., Goldstein, G.W. and Bressler, J.P. (1996) Role of anion exchange and thiol groups in the regulation of potassium efflux by lead in human erythrocytes. J. Cell. Phys. 167, 222-228 
    28. Lou, M., Garay, R. and Aida, J.O. (1991) Cadmium uptake through the anion exchanger in human red blood cells. J. Physiology 443, 123-136 
    29. Lupien, S.J., et al. (2000) Child's stress hormone levels correlate with mother's socioeconomic status and depressive state. Biol. Psychiatry 48(10), 976-980 
    30. Markovich, D. and Murer, H. (2004) The SLC13 gene family of sodium sulphate/carboxylate cotransporters. Pflugers Arch. 447(5), 594-602 
    31. Mount, D.B. and Romero M.F. (2004) The SLC26 gene family of multifunctional anion exchangers. Pflugers Arch. 447(5), 710-721 
    32. Olson, C.M. (1999) Nutrition and health outcomes associated with food insecurity and hunger. J. Nutr. 129(2S Suppl), 521S-524S 
    33. Oomen, A.G., et al. (2003) In vitro intestinal lead uptake and transport in relation to speciation. Arch. Environ. Contam. Toxicol. 44(1), 116-124 
    34. Paschal, D.C., et al. (2003) Exposure of the U.S. population aged 6 years and older to cadmium: 1988- 1994. Arch. Environ. Contam. Toxicol. 38(3), 377-383 
    35. Ragan, H.A. (1977) Effects of iron deficiency on the absorption and distribution of lead and cadmium in rats. J. Lab. Clin. Med. 90(4), 700-706 
    36. Satarug, S., Haswell-Elkins, M.R. and Moore, M.R. (2000) Safe levels of cadmium intake to prevent renal toxicity in human subjects. Br. J. Nutr. 84(6), 791-802 
    37. Schopfer, L.M. and Salhany, J.M. (1995) Characterization of the stilbenedisulfonate binding site on band 3. Biochemistry 34(26), 8320-8329 
    38. Selevan, S.G., et. al. (2003) Blood lead concentration and delayed puberty in girls. N. Engl. J. Med. 348(16), 1527-1536 
    39. Simpson, R.J. and Peters, T.J. (1986) Mouse intestinal Fe3+ uptake kinetics in vivo. The significance of brush-border membrane vesicle transport in the mechanism of mucosal Fe3+ uptake. Biochim. Biophys. Acta., 856(1), 115-122 
    40. Stanek, E.J., 3rd and Calabrese, E.J. (2000) Daily soil ingestion estimates for children at a Superfund site. Risk Anal. 20(5), 627-635 
    41. Stewart, W.F., et al. (1999) Neurobehavioral function and tibial and chelatable lead levels in 543 former organolead workers. Neurology 52(8), 1610-1617 
    42. Sugawara, N. and Sugawara, C. (1991) Gastrointestinal absorption of Cd-metallothionein and cadmium chloride in mice. Arch. Toxicol. 65(8), 689-692 
    43. Trinder, D., et al. (2000) Localization of divalent metal transporter 1 (DMT1) to the microvillus membrane of rat duodenal enterocytes in iron deficiency, but to hepatocytes in iron overload [see comments]. Gut 46(2), 270-276 
    44. Vahter, M., et al. (1996) Bioavailability of cadmium from shellfish and mixed diet in women. Toxicol. Appl. Pharmacol. 136(2), 332-341 
    45. Valberg, L.S., Sorbie, J. and Hamilton, D.L. (1976) Gastrointestinal metabolism of cadmium in experimental iron deficiency. Am. J. Physiol. 231(2), 462-467 
    46. Vulpe, C.D., et al. (1999) Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse. Nat. Genet. 21(2), 195-199 
    47. Calabrese, E.J., et al. (1997) Soil ingestion: a concern for acute toxicity in children. Environ Health Perspect, 1997. 105(12), 1354-1358 
    48. Dufner-Beattie, J., et al. (2003) The Acrodermatitis Enteropathica Gene ZIP4 Encodes a Tissue-specific, Zinc-regulated Zinc Transporter in Mice. J. Biol. Chem. 278(35), 33474-33481 
    49. Bebe, F.N. and Panemangalore, M. (1996) Modulation of tissue trace metal concentrations in weanling rats fed different levels of zinc and exposed to oral lead and cadmium. Nutrition Res. 16(8), 1369-1380 
    50. Crowe, A. and Morgan E.H. (1996) Interactions between tissue uptake of lead and iron in normal and iron-deficient rats during development. Biol. Trace Elem. Res. 52(3), 249-261 
    51. Groten, J.P., Luten, J.B. and van Bladeren, P.J. (1992) Dietary iron lowers the intestinal uptake of cadmium-metallothionein in rats. Eur. J.Pharmacol. 228(1), 23-28 
    52. Klein, D., Greim, H. and Summer K.H. (1986) Stability of metallothionein in gastric juice. Toxicology 41(2), 121-129 
    53. Simons, T.J.B. (1986) The role of anion transport in the passive movement of lead across the human red cell membrane. J. Physiol. (Land) 378, 287-312 
    54. Bevensee, M.O., Apkon, M. and Boron, W.F. (1997a) Intracellular pH regulation in cultured astrocytes from rat hippocampus. II. Electrogenic Na/HCO3 cotransport. J. Gen. Physiol. 110(4), 467-483 
    55. Jarup, L., et al. (2000) Low level exposure to cadmium and early kidney damage: The OSCAR study. Occup. Environ. Med. 57 (10), 668-672 
    56. Ohta, H. and Cherian, M.G. (1991) Gastrointestinal absorption of cadmium and metallothionein. Toxicol. Appl.Pharmacol. 107(1), 63-72 
    57. Romero, M.F., Fulton, C.M. and Boron, W.F. (2004) The SLC4 family of HCO3 - transporters. Pflugers Arch. 447(5), 495-509 

 저자의 다른 논문

  • 김용배 (37)

    1. 1996 "연노출근로자에 있어서 흡광광도법과 HPLC법에 의한 요중 δ-ALA 배설량과 연노출지표들 간의 관련성" 한국산업위생학회지 = Journal of Korean Society of Occupational and environmental hygiene 6 (1): 77~87    
    2. 1996 "연 축전지 사업장의 질환 요주의자 및 유소견자의 사후관리 실태 연구" 예방의학회지 = Korean journal of preventive medicine 29 (4): 733~746    
    3. 1996 "축전지 제조업에서 입사 1년 미만 남자 사원들의 연 노출 지표치에 관한 연구" 예방의학회지 = Korean journal of preventive medicine 29 (4): 747~764    
    4. 1997 "연취급 근로자들의 혈중 ZPP 농도 선별기준에 따른 정확도의 변화" 예방의학회지 = Korean journal of preventive medicine 30 (4): 741~751    
    5. 1998 "흡연과 음주가 연취급 근로자들의 건강수준에 미치는 영향" 예방의학회지 = Korean journal of preventive medicine 31 (4): 708~718    
    6. 1999 "연 폭로시 성별에 따른 혈중 Zinc Protoporphyrin의 양-반응 관계" 예방의학회지 = Korean journal of preventive medicine 32 (4): 499~504    
    7. 2000 "연 노출 근로자들의 혈장 δ - aminolevulinic acid 량과 연 노출 지표들과의 관련성" 한국산업위생학회지 = Journal of Korean Society of Occupational and environmental hygiene 10 (2): 165~172    
    8. 2006 "Effects of the Fermented Milk Intake on Human Antioxidant Activity and Blood Alcohol Concentration" Food science and biotechnology 15 (1): 82~85    
    9. 2007 "두경부암종 수술 후 결손부위 재건에 사용된 유리피판술 51예의 고찰" 대한 두경부 종양 학회지 = Korean journal of head & neck oncology 23 (1): 26~31    
    10. 2007 "욕창 환자에서 자율신경성 반사부전증의 경험례" 大韓成形外科學會誌 = Journal of the Korean society of plastic and reconstructive surgeons 34 (4): 531~534    
  • Ko, Hong-Sook (7)

  • Cheong, Jae-Hoon (46)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기