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Cholinomimetic Properties of a Water-Soluble Fraction from Mulberry Leaves in Rats

Lee, Ju-Seon    (Drug and Toxicology Division, National Institute of Scientific Investigation   ); Chung, Sung-Hyun    (College of Pharmacy, Kyung Hee University   ); Lee, Yong-Sup    (College of Pharmacy, Kyung Hee University   ); Jin, Chang-Bae    (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology  );
  • 초록

    The present study examined effects of a water-soluble fraction from mulberry leaves (ML water fraction) on the circulatory and autonomic nervous systems, which were compared with those of acetylcholine (ACh) used as a reference drug in order to elucidate its mechanism of action. Intravenous administration of ACh or a ML water fraction produced temporary depressor and tachycardiac responses in a dose-dependent manner in unrestrained, conscious Sprague-Dawley rats. The systemic hemodynamic effects of ACh and a ML water fraction were almost completely blocked by pretreatment with atropine, a muscarinic antagonist. The depressor responses to ACh and a ML water fraction were slightly enhanced and prolonged by pretreatment with neostigmine, an anticholinesterase, whereas the tachycardiac responses were remarkably blocked by pretreatment with pentolinium, a ganglionic blocking agent. In vitro experiments using the ileum isolated from rats showed that ACh and a ML water fraction increased ileal contractility in a dose-dependent manner. The increases in ileal contractility were also completely abolished in the presence of atropine. Finally, the specific binding of [ $^3H$ ]quinuclidinyl benzilate, a muscarinic antagonist, to rat cortical synaptic membranes was inhibited by a ML water fraction in a concentration-dependent manner with an IC $_{50}$ value of 9.5 mg/ml. The results suggest that the effects of a ML water fraction are mediated through direct stimulation of muscarinic cholinergic receptors by unknown cholinomimetic substance(s) contained in that fraction.


  • 주제어

    Mulberrry Leaves .   Acetylcholine .   Blood pressure .   Heart rate .   Ileal contractility .   Muscarinic receptor.  

  • 참고문헌 (16)

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