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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.477 - 483   피인용횟수: 2

Thymidine Production by Corynebacterium ammoniagenes Mutants

Song, Kyung-Hwa    (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies   ); Kwon, Do-Young    (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies   ); Kim, Sang-Yong    (BioNgene Co., Ltd.   ); Lee, Jung-Kul    (BioNgene Co., Ltd.   ); Hyun, Hyung-Hwan    (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies  );
  • 초록

    Corynebacterium ammoniagenes ATCC 6872, which does not accumulate pyrimidine nucleoside or nucleotide, was metabolically engineered to secrete a large amount of thymidine. Characteristics of 5-fluorouracil resistance ( $FU^r$ ), hydroxyurea resistance ( $HU^r$ ), trimethoprim resistance ( $TM^r$ ), thymidylate phosphorylase deficiency ( $deoA^-$ ), inosine auxotrophy ( $ino^-$ ), 5-fluorocytosine resistance ( $FC^r$ ), thymidine kinase deficiency, and thymidine resistance ( $thym^r$ ) were successively introduced into mutant strains KR3 and DY5T9-5, and shake-flask cultures were able to accumulate 408.1 mg/l and 428.2 mg/l of thymidine, respectively, as a major product. The mutant strains did not accumulate thymine at all and accumulated less than 10 mg/l of other pyrimidine nucleosides, such as cytosine, cytidine, and deoxycytidine, as byproducts.


  • 주제어

    Corynebacterium ammoniagenes .   thymidine .   pyrimidine .   metabolic engineering.  

  • 참고문헌 (13)

    1. Freisheim J. H., C. C. Smith, and P. M. Guzy. 1972. Dihydrofolate reductase and thymidylate synthetase in strains of Streptococcus faecium resistant to pyrimethamine, chlorguanide triazine, trimethoprim, and amethopterin. Arch. Biochem. Biophys. 148: 1-9 
    2. Neuhard, J. 1983. Utilization of preformed pyrimidine bases and nucleoside, pp. 95-148. In Munch-Petersen, A. (ed.) Metabolism of Nucleotides, Nucleosides and Nucleobases in Microorganism. Academic Press, London 
    3. Roland, K. L., F. E. Powell, and C. L. Jr. Turnbough. 1985. Role of translation and attenuation in the control of pyrBl operon expression in Escherichia coli K-12. J. Bacteriol. 163: 991-999 
    4. Potvin, B. W., R. J. Jr. Kelleher, and H. Gooder. 1975. Pyrimidine biosynthetic pathway of Bacillus subtilis. J. Bacteriol. 123: 604-615 
    5. Timson J. 1975. Hydroxyurea. Mutat. Res. 32: 115-132 
    6. Hammer, J. K. 1983. Nucleotide catabolism, pp. 203-258 In Munch-Petersen, A. (ed.), Metabolism of Nucleotides, Nucleosides and Nucleobases in Microorganism. Academic Press, London 
    7. Schwartz, M. 1976. Thymidine phosphorylase from Escherichia coli, pp. 442-443. In A. H. Patricia and E. J. Mary (eds.). Methods in Enzymology, vol. 51, Academic Press, Avenue, New York, U.S.A 
    8. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. BioI. Chem. 193: 265-275 
    9. Scocca, J. J. 1971. Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus irifluenzae. J. BioI. Chem. 246: 6606-6610 
    10. Tsen, S. D. 1994. Chemostat selection of Escherichia coli mutants secreting thymidine, cytosine, uracil, guanine, and thymine. Appl. Microbiol. Biotechnol. 41: 232-238 
    11. Santi, D. V. and C. S. McHenry. 1972. 5-Fluoro-2'deoxyuridylate: Covalent complex with thymidylate synthetase. Proc. Natl. Acad. Sci. USA 69: 1855-1857 
    12. Saunders P. P., B. A. Wilson, and G. F. Saunders. 1969. Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus. J. BioI. Chem. 244: 3691-3697 
    13. Asahi, S. and Y. Tsueni. 1989. Method for production of cytidine and/or deoxycytidine. US patent 4,839,285 
  • 이 논문을 인용한 문헌 (2)

    1. 2007. "" Journal of microbiology and biotechnology, 17(6): 1010~1017     
    2. 2011. "" The journal of microbiology, 49(1): 53~57     

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