본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.484 - 490   피인용횟수: 3

DNA Repair Activity of Human rpS3 is Operative to Genotoxic Damage in Bacteria

JANG CHANG-YOUNG    (Laboratory of Biochemistry, School of Life Sciences & Biotechnology, and Bioinstitute, Korea University   ); LEE JAE YUNG    (Department of Biology, Mokpo National University   ); KIM JOON    (Laboratory of Biochemistry, School of Life Sciences & Biotechnology, and Bioinstitute, Korea University  );
  • 초록

    Human ribosomal protein S3 (rpS3), which has a DNA repair endonuclease activity, is a multifunctional protein. This protein is involved in DNA repair, translation, and apoptosis. In particular, rpS3 has a lyase activity, which cleaves the phosphodiester bond of damaged sites such as cyclobutane pyrimidine dimers and AP sites. Here, using deletion analysis, we identified that the repair endonuclease domain resides in the C-terminal region (165-243 aa) of rpS3. We also found that ectopic expression of GST-rpS3 in bacterial strain BL21 promoted the resistance of these cells to ultraviolet (UV) radiation and hydrogen peroxide ( $H_{2}O_{2}$ ) treatment. The repair domain of rpS3 was sufficient to exhibit the resistance to UV irradiation and recover cell growth and viability, showing that the repair activity of rpS3 is responsible for the resistance to UV irradiation. Our study suggests that rpS3 is able to process DNA damage in bacteria via its repair domain, showing the resistance to genotoxic stress. This implies that rpS3-like activity could be operative in bacteria.

  • 주제어

    rpS3 .   DNA repair .   repair domain .   endonuclease .   UV resistance.  

  • 참고문헌 (38)

    1. Lim, Y., S. M. Lee, J. Y. Lee, E. P. Moon, B. J. Lee, and J. Kim. 2002. Complete genomic structure of human rpS3: Identification of functional U 15b snoRNA in the fifth intron. Gene 286: 291-297 
    2. Naora, H.,l. Takai, M. Adachi, and H. Naora. 1998. Altered cellular responses by varying expression of a ribosomal protein gene: Sequential coordination of enhancement and suppression of ribosomal protein S3a gene expression induces apoptosis. J. Cell Biol. 141: 741-753 
    3. Neumann, F. and U. Krawinkel. 1997. Constitutive expression of human ribosomal protein L7 arrests the cell cycle in G1 and induces apoptosis in Jurkat T-Iymphoma cells. Exp. Cell Res. 230: 252-261 
    4. Slupphaug, G., B. Kavli, and H. E. Krokan. 2003. The interacting pathways for prevention and repair of oxidative DNA damage. Mutat. Res. 531: 231-251 
    5. Thayer, M. M., H. Ahern, D. Xing, R. P. Cunningham, and J. A. Tainer. 1995. Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure. EMBO J. 14: 4108-4120 
    6. Tudek, B. 2003. Oxidative DNA damage and its repair; Base excision repair. Mutat. Res. 531: 1-3 
    7. Ziemiecki, A., R. G. Muller, F. Xiao-Chang, N. E. Hynes, and S. Kozma. 1990. Oncogenic activation of the human trk proto-oncogene by recombination with the ribosomal large subunit protein L7a. EMBO J. 9: 191-196 
    8. Gu, L., S. M. Huang, and M. Sander. 1993. Drosophila Rrp1 complements E. coli xth nfo mutants: Protection against both oxidative and alkylation-induced DNA damage. Nucleic Acids Res. 21: 4788-4793 
    9. Joo, Y.-J., H.-J. Kim, J. Y. Lee, and J. Kim. 2004. Biochemical quantitation of PM2 page DNA as a substrate for endonuclease assay. J. Microbiol. 42: 99-102 
    10. Kim, S. H., J. T. Lee, and J. Kim. 2005. Characterization of a wide range base-damage endonuclease activity of mammalian rpS3. Biochem. Biophys. Res. Commun. 328: 962-967 
    11. You, Y. H. and P. G. P. Feifer. 2001. Similarities in sunlightinduced mutational spectra of CpG-methylated transgenes and the p53 gene in skin cancer point to an important role of 5-methylcytosine residues in solar UV mutagenesis. J. Mol. Biol. 305: 389-399 
    12. Jeon, K. S., E. J. Geun, and I. K. Hwang, 2002. Assay of ${\beta}$-glucosidase activity of bifidobacteria and the hydrolysis of isoflavone glycosides by Bifidobacterium sp. Int-57 in soymilk fermentation. J. Microbiol. Biotechnol. 12: 8-13 
    13. Kastan, M. B., O. Onyekwere, D. B. V. Sidransky, and R. W. Craig. 1991. Participation of p53 protein in the cellular response to DNA damage. Cancer Res. 51: 6304-6311 
    14. Yarosh, D. B. 2002. Enhanced DNA repair of cyclobutane pyrimidine dimers changes the biological response to UV-B radiation. Mutat. Res. 509: 221-226 
    15. Bommer, U. A., G. Lutsch, J. Stahl, and R. Bielka. 1991. Eukaryotic initiation factors eIF-2 and eIF-3: Interactions, structure and localization in ribosomal initiation complexes. Biochimie (Paris) 73: 1007-1019 
    16. Yacoub, A., L. Augeri, M. R. Kelley, P. W. Doetsch, and W. A. Deutsch. 1996. A Drosophila ribosomal protein contains 8-oxoguanine and a basic site for DNA repair activities. EMBO J. 15: 2306-2312 
    17. Hegde, V., M. R. Kelley, Y. Xu, I. S. Mian, and W. A. Deutsch. 2001. Conversion of the bifunctional 8-oxoguanine/beta-delta apurinic/apyrimidinic DNA repair activities of Drosophila ribosomal protein S3 into the human S3 monofunctional beta-elimination catalyst through a single amino acid change. J. Biol.Chem. 276: 27591-27596 
    18. Jang, C.-Y., J. Y. Lee, and J. Kim. 2004. RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis. FEBS Lett. 561: 81-85 
    19. Sandigursky, M., A. Yacoub, M. R. Kelley, W. A. Deutsch, and W. A. Franklin. 1997. The Drosophila ribosomal protein S3 contains a DNA deoxyribophosphodiesterase (dRpase) activity. J. Biol. Chem. 272: 17480-17484 
    20. Kim, J., L. S. Chubatsu, A. Admon, J. Stahl, R. Fellous, and S. Linn. 1995. Implication of mammalian ribosomal protein S3 in the processing of DNA damage. J. BioI. Chem. 270: 13620-13629 
    21. Hegde, V., M. R. Kelley, Y. Xu, I. S. Mian, and W. A. Deutsch. 2001. Conversion of the bifunctional 8-oxoguanine/${\beta}-{\delta}$ apurinic/apyrimidinic DNA repair activities of Drosophila ribosomal protein S3 into the human S3 monofunctional ${\beta}$-elimination catalyst through a single amino acid change. J. BioI. Chem. 276: 27591-27596 
    22. Friedberg, E. C. 2001. How nucleotide excision repair protects against cancer. Nat. Rev. Cancer 1: 22-33 
    23. Naora, H. and H. Naora. 1999. Involvement of ribosomal proteins in regulating cell growth and apoptosis: Translational modulation or recruitment for extraribosomal activity? lmmunol. Cell BioI. 77: 197-205 
    24. Wilson, D. M., W. A. Deutsch, and M. R. Kelley. 1994. Drosophila ribosomal protein S3 contains an activity that cleaves DNA at apurinic/apyrimidinic sites. J. Biol. Chem. 269:25359-25364 
    25. Baik, S. H., P. K. Cho, M. H. Kim, and S. E. Yun. 2003. Enhanced production of benzoylformate reductase in Enterococcus faecalis under oxidative stress established by natural electron carriers. J. Microbiol. Biotechnol. 13: 100-104 
    26. Christmann, M., M. T. Tomicic, W. P. Roos, and B. Kaina. 2003. Mechanisms of human DNA repair: An update. Toxicology 193: 3-34 
    27. Jung, S. O., C. K. Auh, J. C. Ryu, and J. Kim. 2002. A new method for substrate preparation of PM2 phage DNA for endonuclease assay. Antonie van Leeuwenhoek 83: 223-229 
    28. Kim, J. and S. Linn, 1989. Purification and characterization of UY endonucleases from murine plasmacytoma cells. J. BioI. Chem. 264: 2739-2745 
    29. Kuo, C. F., D. E. McRee, C. L. Fisher, S. F. O'Handley, R. P. Cunningham, and J. A. Tainer. 1992. Atomic structure of the DNA repair [4Fe-4S] enzyme endonuclease III. Science 258: 434-440 
    30. Kuznetsov, S. V., O. M. Sidorkina, J. Laval, and A. Ansari. 2001. Characterization of thermal stability of the Escherichia coli FapyDNA glycosylase. Biochem. Biophys. Res. Commun. 288: 121-128 
    31. Van Lijesbettens, M., R. Vanderhaeghen, M. De Block, G. Bauw, R. Villarroel, and M. Van Montagu. 1994. An S18 ribosomal protein gene copy at the Arabidopsis PFL locus affects plant development by its specific expression in meristems. EMBO J. 13: 3378-3388 
    32. Esther, E. A. V., M. van Kesteren, J. J. Turner, G. A. van der Marel, J. H. van Boom, G. F. Moolenaar, and N. Goosen. 2002. The C-terminal region of Escherichia coli UvrC contributes to the flexibility of the UvrABC nucleotide excision repair system. Nucleic Acids Res. 30: 2492-2500 
    33. Scharer, O. D. and J. Jiricny. 2001. Recent progress in the biology, chemistry and structural biology of DNA glycosylases. Bioessays 23: 270-281 
    34. Chen, D. S., C. Law, and P. Keng. 1993. Reduction of radiation cytotoxicity by human apurinic endonuclease in a radiation-sensitive Escherichia coli mutant. Radiat. Res. 135: 405-410 
    35. Min, J. and M. B. Gu. 2003. Genotoxicity assay using chromosomally-integrated bacterial recA::Lux. J. Microbiol. Biotechnol. 13: 91-99 
    36. Atri, N. and L. C. Rai. 2003. Differential responses of three cyanobacteria to UV-B and Cd. J. Microbiol. Biotechnol. 13: 538-544 
    37. Deutsch, W. A., A. Yacoub, P. Jaruga, T. H. Zastawny, and M. Dizdaroglu. 1997. Characterization and mechanism of action of Drosophila ribosomal protein S3 DNA glycosylase activity for the removal of oxidative damaged DNA bases. J. BioI. Chem. 272: 32857-32860 
    38. Friedberg, E. C. 2003. DNA damage and repair. Nature 42: 436-440 
  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of microbiology and biotechnology, 16(10): 1583~1590     
    2. 2006. "" Journal of microbiology and biotechnology, 16(7): 1144~1148     
    3. 2007. "" Journal of microbiology and biotechnology, 17(8): 1390~1393     

 저자의 다른 논문

  • Jang, Chang-Young (2)

    1. 2002 "Multiple functions of human UV DNA repair endonuclease III" Journal of photoscience : an international journal officail organ of the korean society of photoscience 9 (2): 182~185    
    2. 2003 "Human rpS3 is involved in DNA repair and cell cycle control" Journal of photoscience : an international journal officail organ of the korean society of photoscience 10 (2): 195~198    
  • 이재영 (10)

  • Kim, Joon (20)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기