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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.491 - 496   피인용횟수: 5

Isolation and Characterization of Kasugamycin Biosynthetic Genes from Streptomyces kasugaensis KACC 20262

JO YOU-YOUNG    (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University   ); LIU JING    (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University   ); JIN YING-YU    (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University   ); YANG YOUNG-YELL    (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University   ); SUH JOO-WON    (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University  );
  • 초록

    The biosynthetic gene cluster for the aminoglycoside antibiotic kasugamycin was isolated and characterized from the kasugamycin producing strain, Streptomyces kasugaensis KACC 20262. By screening a fosmid library using kasA, the gene encoding aminotransferase, we isolated a 22 kb DNA fragment. The fragment contained seventeen complete open reading frames (ORFs); one of these ORFs, kasD, was identified as the gene for dNDP-glucose 4,6-dehydratase, which catalyzes the conversion of dNDP-glucose to 4-keto-6-deoxy-dNDP-glucose. The enzyme showed a broad spectrum of substrate specificity. In addition, ksR was overexpressed in E. coli BL21 and proved to be a self-resistance gene against kasugamycin. These findings suggest that the isolated gene cluster is highly likely responsible for the biosynthesis of kasugamycin.


  • 주제어

    Kasugamycin .   biosynthetic gene cluster .   dNDP-glucose-4,6-dehydratase .   resistance gene.  

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