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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.532 - 536   피인용횟수: 6

Gentisyl Alcohol Inhibits Apoptosis by Suppressing Caspase Activity Induced by Etoposide

KIM JINHEE    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); KIM DONGHYUN    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); KIM MEEREE    (Department of Food and Nutrition, Chungnam National University   ); KWON HOJEONG    (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University   ); OH TAEKWANG    (Microbial Genomics and Applications Center, Korea Research Institute of Bioscience and Biotechnology   ); LEE CHOONGHWAN    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide ( $10\;{\mu}g/ml$ ), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with $IC_{50}$ value of $3.0\;{\mu}g/ml$ after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.


  • 주제어

    Apoptosis .   U937 cells .   gentisyl alcohol.  

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  • 이 논문을 인용한 문헌 (6)

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