본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.532 - 536   피인용횟수: 6

Gentisyl Alcohol Inhibits Apoptosis by Suppressing Caspase Activity Induced by Etoposide

KIM JINHEE    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); KIM DONGHYUN    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology   ); KIM MEEREE    (Department of Food and Nutrition, Chungnam National University   ); KWON HOJEONG    (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University   ); OH TAEKWANG    (Microbial Genomics and Applications Center, Korea Research Institute of Bioscience and Biotechnology   ); LEE CHOONGHWAN    (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide ( $10\;{\mu}g/ml$ ), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with $IC_{50}$ value of $3.0\;{\mu}g/ml$ after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

  • 주제어

    Apoptosis .   U937 cells .   gentisyl alcohol.  

  • 참고문헌 (24)

    1. Bhunia, A. K. and X. Feng. 1999. Examination of cytopathic effect and apoptosis in Listeria monocytogenes-infected hybridoma B-Iymphocyte (Ped-2E9) line in vitro. J. Microbiol. Biotechnol. 9: 398-403 
    2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
    3. Kim, J. W., H. Adachi, K. Shin-Ya, Y. Hayakawa, and H. Seto. 1997. Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp. J. Antibiot. 50: 628-630 
    4. Nicholson, D. W., A. Ali, N. A. Thornberry, J. P. Vaillancourt, C. K. Ding, M. Gallant, Y. Gareau, P. R. Griffin, M. Labelle, and Y. A. Lazebnik. 1995. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43 
    5. Yamashita, N., K. Shin- Ya, K. Furihata, Y. Hayakawa, and H. Seto. 1998. New ravidomycin analogues, FE35A and FE35B, apoptosis inducers produced by Streptomyces rochei. J. Antibiot. 51: 1105 -1108 
    6. Jeong, Y. O., K. S. Kim, J. Y. Oh, J. C. Park, J. H. Bang, S. W. Choi, and J. C. Lee. 2003. Growth inhibition and apoptosis induction of gastric cancer cells by copper glycinate complex. J. Microbiol. Biotechnol. 13: 394-399 
    7. Lee, C. H., M. A. Lee, Y. H. Cho, H. Y. Lim, K. H. Kim, and Y. H. Lim. 2001. In vitro detection of apoptosis in human promyelocytic leukemia HL-60 cells by $^1H$-NMR. J. Microbiol. Biotechnol. 11: 539-542 
    8. Lee, C. H., J. H. Kim, H. J. Lee, and Y. H. Kho. 2001. Two new constituents of Isodon excisus and their evaluation in an apoptosis inhibition assay. J. Nat. Prod. 64: 659-660 
    9. Wiley, S. R., K. Schooley, P. J. Smolak, W. S. Din, C. P. Huang, J. K. Nicholl, G. R. Sutherland, T. D. Smith, C. Rauch, and C. A. Smith. 1995. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 3: 673-682 
    10. Lademann, U., K. Cain, M. Gyrd-Hansen, D. Brown, D. Peters, and M. Jattela. 2003. Diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodaIton apoptosome complex. Mol. Cell Biol. 23: 7829-7837 
    11. Cavalieri, E., S. Mariotto, C. Fabrizi, A. C. Prati, R. Gottardo, S. Leone, L. V. Berra, G. M. Lauro, A. R. Ciampa, and H. Suzuki. 2004. alpha-Bisabolol, a nontoxic natural compound, strongly induces apoptosis in glioma cells. Biochem. Biophys. Res. Commun. 315: 589-594 
    12. Miura, M., H. Zhu, R. Rotello, E. A. Hartwieg, and J. Yuan. 1993. Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3. Cell 75: 653-660 
    13. Goodwin, C. J., S. J. Holt, S. Downes, and N. J. Marshall. 1995. Microculture tetrazolium assays: A comparison between two new tetrazolium salts, XTT and MTS. J. Immunol. Methods 179: 95-103 
    14. Meier, P. and G. Evan. 1998. Dying like flies. Cell 95: 295-298 
    15. Kakeya, H., H. P. Zhang, K. Kobinata, R. Onose, C. Onozawa, T. Kudo, and H. Osada. 1997. Cytotrienin A, a novel apoptosis inducer in human leukemia HL-60 cells. J. Antibiot. 50: 370-372 
    16. Bae, M. A., K. Yamada, D. Uemura, J. H. Seu, and Y. H. Kim. 1998. Aburnatubolactam C, a novel apoptosis-inducing substance produced by marine Streptomyces sp. SCRC A-20. J. Microbiol. Biotechnol. 8: 455-460 
    17. Ishikawa, Y., K. Morimoto, and T. Hamasaki. 1986. Gentisyl alcohol produced by Penicillium commune, a potent antioxidant and synergist for tocopherol. J. Jpn. Oil Chem. Soc. 35: 13-16 
    18. Alfaro, C., A. Urios, M. C. Gonzalez, P. Moya, and M. Blanco. 2003. Screening for metabolites from Penicillium novae-zeelandiae displaying radical-scavenging activity and oxidative mutagenicity: Isolation of gentisyl alcohol. Mutat. Res. 539: 187-194 
    19. Ji, L., G. Zhang, and Y. Hirabayashi. 1995. Inhibition of tumor necrosis factor alpha- and ceramide-induced internucleosomal DNA fragmentation by herbimycin A in U937 cells. Biochem. Biophys. Res. Commun. 212: 640-647 
    20. Fernandes-Alnemri, T., G. Litwack, and E. S. Alnemri. 1994. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269: 30761-30764 
    21. Steller, H. 1995. Mechanisms and genes of cellular suicide. Science 267: 1445-1449 
    22. Gurtu, V., S. R. Kain, and G. Zhang. 1997. Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal. Biochem. 251: 98-102 
    23. Dove, A. 2001. Making a living out of the art of dying. Nat. Biotechnol. 19: 615-619 
    24. Guo, Y. and N. Kyprianou. 1999. Restoration of transforming growth factor beta signaling pathway in human prostate cancer cells suppresses tumorigenicity via induction of caspase-1-mediated apoptosis. Cancer Res. 59: 1366-1371 
  • 이 논문을 인용한 문헌 (6)

    1. 2006. "" Journal of microbiology and biotechnology, 16(3): 475~479     
    2. 2006. "" Journal of microbiology and biotechnology, 16(8): 1262~1268     
    3. 2007. "" Journal of microbiology and biotechnology, 17(11): 1856~1861     
    4. 2007. "" Journal of microbiology and biotechnology, 17(4): 638~643     
    5. 2008. "" Journal of microbiology and biotechnology, 18(3): 523~531     
    6. 2009. "" Journal of microbiology and biotechnology, 19(9): 911~917     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기