본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.560 - 567   피인용횟수: 2

Characterization of Exopolysaccharides Produced by Submerged Culture of an Entomopathogenic Fungus Paecilomyces sinclairii by Using a Multi-Angle Laser Light Scattering System

KIM SANG-WOO    (Department of Biotechnology, Daegu University   ); HWANG HYE-JIN    (Department of Biotechnology, Daegu University   ); CHOI JANG-WON    (Department of Bioindustry, Daegu University   ); YUN JONG-WON    (Department of Biotechnology, Daegu University  );
  • 초록

    Three groups of exopolysaccharides (EPSs) (designated as Fr-I, Fr-II, and Fr-III) were isolated from the culture filtrates of Paecilomyces sinclairii by gel filtration chromatography on Sepharose CL-4B. Their molecular characteristics were examined by multi-angle laser light scattering (MALLS) connected online to a size exclusion chromatography (SEC) and refractive index (RI) detector system. The weight-average molar mass of Fr-I, Fr-II, and Fr-III of EPSs were determined to be $1.540{\times}10^6,\;6.302{\times}10^4$ , and $9.389{\times}10^4\;g/mol$ , respectively. All three EPSs showed a fairly low polydispersity indice, ranging from 1.008 to 1.059 (nearly mono disperse behavior), and showed different carbohydrates and amino acids compositions; all fractions of EPSs consisted of mainly cystine, valine, and arginine in the protein moiety, and mainly ribose, galactose, and glucose in the carbohydrate moiety. The determination of gyration radii of the EPSs in SEC/MALLS analysis revealed the molecular shape of the Fr-I to be a rod-like structure, whereas the Fr-II and Fr-III had a random-coil structure in an aqueous solution.


  • 주제어

    Exopolysaccharides .   MALLS .   fungus .   Paecilomyces sinclairii.  

  • 참고문헌 (34)

    1. Chihara, G., J. Himuri, Y. Y. Maeda, Y. Arai, and F. Fukuoka. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing. (an edible mushroom). Cancer Res. 30: 2776-2781 
    2. Jeong, S. C., S. P. Cho, B. K. Yang, Y. T. Jeong, K. S. Ra, and C. H. Song. 2004. Immunomodulating activity of the exopolymer from submerged mycelial culture of Phellinus pini. J. Microbiol. Biotechnol. 14: 15-21 
    3. Kim, H. H., J. G. Na, Y. K. Chang, G. T. Chun, S. J. Lee, and Y. H. Jeong. 2004. Optimization of submerged culture conditions for mycelial growth and exopolysaccharides production by Agaricus blazei. J. Microbiol. Biotechnol. 14: 944-951 
    4. Knobloch, J. E. and P. N. Shaklee. 1997. Absolute molecular weight distribution of low-molecular-weight heparins by size-exclusion chromatography with multiangle laser light scattering detection. Anal. Biochem. 245: 231-241 
    5. Park, Y. H., E. K. Moon, Y. K. Shin, M. A. Bae, J. K. Kim, and Y. H. Kim. 2000. Antitumor activity of Paecilomyces japonica is mediated by apoptotic cell death. J. Microbiol. Biotechnol. 10: 16-20 
    6. Song, C. H., Y. J. Jeon, B. K. Yang, K. S. Ra, and J. M. Sung. 1998. The anti-complementary activity of exo-polymers produced from submerged mycelial cultures of higher fungi with particular reference to Cordyceps militaris. J. Microbiol. Biotechnol. 8: 536-539 
    7. Tabata, K., W. Itoh, T. Kojima, S. Kawabate, and K. Misaki. 1981. Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune FRIES. Carbohydr. Res. 89: 121-135 
    8. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60: 258-274 
    9. Xu, C. P., S. W. Kim, H. J. Hwang, and J. W. Yun. 2002. Application of statistically-based experimental designs for the optimization of the exo-polysaccharide production by Cordyceps militaris NG3. Biotechnol. Appl. Biochem. 36: 127-131 
    10. Yokoyama, W., J. J. Renner-Nantz, and C. F. Shoemaker. 1998. Starch molecular mass and size by size-exclusion chromatography in DMSO-LiBr coupled with multiple angle laser light scattering. Cereal Chem. 75: 530-535 
    11. Kim, S. W., H. J. Hwang, C. P. Xu, Y. S. Na, S. G. Song, and J. W. Yun. 2002. Influence of nutritional conditions on the mycelial growth and exopolysaccharide production in Paecilornyces sinclairii. Lett. Appl. Microbiol. 34: 389-393 
    12. Picton, L., G. Mocanu, D. Mihai, A. Carpov, and G. Muller. 1995. Chemically modified exopolysaccharide pullulans: Physico-chemical characteristics of ionic derivatives. Carbohydr. Polym. 28: 131- 136 
    13. Debye, P. 1947. Molecular-weight determination by light scattering. J. Phys. Coli. Chem. 51: 18-32 
    14. Zimm B. H. 1948. The scattering of light and the radial distribution function of high polymer solutions. J. Chem. Phys. 16: 1093-1099 
    15. Park, J. P., S. W. Kim, H. J. Hwang, and J. W. Yun. 2001. Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris. Lett. Appl. Microbiol. 33: 76-81 
    16. Yoo, S. H. and J. L. Jane. 2002. Molecular weights and gyration radii of amylopectins determined by high-performance size-exclusion chromatography equipped with multi-angle laser-light scattering and refractive index detectors. Carbohydr. Polym. 49: 307-314 
    17. Sone, Y., R. Okuda, N. Wada, E. Kishida, and A. Misaki. 1985. Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum. Agric. Biol. Chem. 49: 2641-2653 
    18. Jong, S. C. and J. M. Birmingham. 1992. Medicinal benefits of the mushroom Ganoderma. Adv. Appl. Microbiol. 37: 101-134 
    19. Wyatt, P. J. 1993. Light scattering and absolute characterization of macromolecules. Anal. Chim. Acta 272: 1-40 
    20. Hanselmann, R., M. Ehrat, and H. M. Widmer. 1995. Sedimentation field flow fractionation combined with multi angle laser light scattering applied for characterization of starch polymers. Starch/Staerke 46: 345-349 
    21. Rouhier, P. M., M. Bruneteau, and G. Nichel. 1995. Structure analysis on ${\beta}$-glucans from Phythophthora capsid. J. Carbohydr. Chem. 14: 247-254 
    22. Yang, B. K., M. A. Wilson, K. Y. Cho, and C. H. Song. 2004. Hypoglycemic effect of exo- and endopolymers produced in submerged mycelial culture of Ganoderma lucidum in Streptozotocin-induced diagetic rats. J. Microbiol. Biotechnol. 14: 972-977 
    23. Bradford, M. H. 1996. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
    24. Yu, K. W., K. S. Shin, Y. M. Choi, and H. J. Suh. 2004. Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran. J. Microbiol. Biotechnol. 14: 658-664 
    25. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugar and related substance. Anal. Chem. 28: 350-356 
    26. Jumel, K., I. Fiebrig, and E. Harding. 1996. Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi laser light scattering. Int. J. Biol. Macromol. 18: 133-139 
    27. Kim, S. Y., S. K. Park, H. K. Park, and S. W. Kim. 1994. Compositional sugar analysis of antitumor polysaccharides by high perfomance liquid chromatography and gas chromatography. Arch. Pharm. Res. 17: 337-342 
    28. Kiho, T., A. Yamane, J. Hui, S. Usui, and S. Ukai. 1996. Polysaccharides in fungi. XXXVI. Hypoglycemic activity of polysaccharide (CF-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol. Pharm. Bull. 19: 294-296 
    29. Aberle, T., W. Burchard, W. Vorwerg, and S. Radosta. 1994. Conformational contributions of amylose and amylopectin to the structural properties of starches from various sources. Starch/Staerke 46: 329-335 
    30. Adachi, Y., N. Ohno, M. Ohsawa, S. Oikawa, and T. Yadomae. 1990. Change of biological activities of (1-3)beta-D-glucan from Grifolafrondosa upon molecular weight reduction by heat treatment. Chem. Pharm. Bull. 38: 477-481 
    31. Turquois, T. and H. Gloria. 2000. Determination of the absolute molecular weight averages and molecular weight distribution of alginates used as ice cream stabilizer by using multiangle laser light scattering. J. Agric. Food Chem. 48: 5455-5458 
    32. Berry, G. C. 1966. Thermodynamic and conformational properties of polystyrene. I. Light-scattering studies on dilute solutions of linear polystyrenes. J. Chem. Phys. 44: 4550-4564 
    33. Capron, I., G. Brigand, and G. Muller. 1997. About the native and renatured conformation of xanthan exopolysaccharide. Polymer 21: 5289-5295 
    34. Fredheim, G. E., S. M. Braaten, and B. E. Christensen. 2002. Molecular weight determination of lignosulfonates by size-exclusion chromatography and multi-angle laser light scattering. J. Chromatogr. A 942: 191-199 
  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" Journal of microbiology and biotechnology, 16(8): 1240~1247     
    2. 2009. "" Journal of microbiology and biotechnology, 19(4): 416~423     

 저자의 다른 논문

  • KIM SANG-WOO (3)

    1. 2004 "Phellinus baumii으로부터 세포외 다당체 생산의 최적화" 생명과학회지 = Journal of life science 14 (1): 51~56    
    2. 2006 "Influence of Agitation Intensity and Aeration Rate on Production of Antioxidative Exopolysaccharides from Submerged Mycelial Culture of Ganoderma resinaceum" Journal of microbiology and biotechnology 16 (8): 1240~1247    
    3. 2008 "Chitosan Oligosaccharides Inhibit Adipogenesis in 3T3-L1 Adipocytes" Journal of microbiology and biotechnology 18 (1): 80~87    
  • HWANG HYE-JIN (3)

  • Choi, Jang-Won (25)

  • Yun, Jong-Won (23)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기