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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.573 - 578   피인용횟수: 3

Purification and Characterization of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans

YU, Tae-Shick   (Department of Microbiology, Keimyung UniversityUU0000145  );
  • 초록

    Pyrimidine nucleotide N-ribosidase (pyrimidine 5'-nucleotide phosphoribo(deoxyribo)hydrolase/pyrimidine 5'-nucleotide nucleosidase, EC 3.2.2.10) catalyzes the breakdown of pyrimidine 5'-nucleotide into pyrimidine base and ribose(deoxyribo)-5-phosphate. However, detailed characteristics of the enzyme have not yet been reported. The enzyme was purified to homogeneity 327.9-fold with an overall yield of $6.1\%$ from Pseudomonas oleovorans ATCC 8062. The enzyme catalyzed cytidine monophosphate (CMP) and uridine monophosphate (UMP), but not adenosine monophosphate (AMP) and guanosine monophosphate (GMP). The enzyme optimally metabolized CMP at pH 6.0 and UMP at around 8.5, and the optimum temperature for the overall enzyme reaction was found to be $37^{\circ}C$ . The $K_m$ values of the enzyme for CMP (at pH 6.0) and UMP (at pH 8.5) were 1.6 mM and 1.1 mM, respectively. AMP, deoxyCMP, and deoxyUMP were very effective inhibitors of the reaction. Double-reciprocal plots obtained in the absence and in the presence of AMP revealed that this inhibitory effect was of the mixed competitive type with respect to the breakdown of CMP and of the noncompetitive type with respect to the breakdown of UMP. In the presence of AMP, the enzyme followed sigmoid kinetics with respect to each substrate.


  • 주제어

    Pyrimidine nucleotide N-ribosidase .   Pseudomonas oleovorans.  

  • 참고문헌 (15)

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    13. Kim, J. and T. S. Yu. 2004. Purification and properties of intracellular cytosine deaminase from Chromobacterium violaceum YK 391. J. Microbiol. Biotechnol. 14: 1182-1189 
    14. Neuhard, J. 1968. Pyrimidine nucleotide methabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium. J. Bacteriol. 96: 1519-1527 
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  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of microbiology and biotechnology, 16(7): 1144~1148     
    2. 2007. "" Journal of microbiology and biotechnology, 17(12): 2027~2032     
    3. 2007. "" Journal of microbiology and biotechnology, 17(9): 1504~1512     

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