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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.579 - 586   피인용횟수: 1

Intracellular Responses of Antibody-Producing H69K-NGD Transfectoma Subjected to Hyperosmotic Pressure

Bae, Sung-Won    (Division of Cardiology, Samsung Medical Center & Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine   ); Lee, Gyun-Min    (Department of Biological Sciences, Korea Advanced Institute of Science and Technology  );
  • 초록

    When subjected to hyperosmotic pressure by NaCl addition, H69K-NGD transfectoma, like KR12H-2 transfectoma, displayed decreased specific growth rate ( ${\mu}$ ) and increased specific antibody productivity ( $q_{Ab}$ ): Elevation of medium osmolality from 280 mOsm/kg to 415 mOsm/kg decreased ${\mu}$ by $79\%$ in batch cultures of H69K-NGD transfectoma, while it increased $q_{Ab}$ by $103\%$ . However, unlike KR12H-2 tranfectoma, enhanced $q_{Ab}$ of H69K-NGD transfectoma at hyperosmolalities was not due to elevated levels of Ig mRNAs. In hyperosmotic cultures of H69K-NGD transfectoma, heavy-chain mRNA per cell was not enhanced with increasing osmolality. Hyperosmotic pressure was found to preferentially enhance immunoglobulin (Ig) translation rates of H69K-NGD transfectoma. However, under hyperosmotic pressure, the translation rate of Ig polypeptides was not enhanced as much as $q_{Ab}$ . This result suggests that hyperosmotic pressure also influences the post-translational process. Taken together, the results obtained show that intracellular response of transfectomas to hyperosmotic pressure, in regard to the main intracellular steps of the antibody secretory pathway, is cell-line dependent.


  • 주제어

    Antibody .   hyperosmotic pressure .   transfectoma.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2007. "" Journal of microbiology and biotechnology, 17(6): 1036~1040     

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