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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.603 - 608   피인용횟수: 2

Safety Assessment of Lactobacillus fermentum PL9005, a Potential Probiotic Lactic Acid Bacterium, in Mice

PARK JONG-HWAN    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University   ); LEE YEONHEE    (Department of Biology, Seoul Women's University   ); MOON ENPYO    (Department of Biology, A-jou University   ); SEOK SEUNG-HYEOK    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University   ); BAEK MIN-WON    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University   ); LEE HUI-YOUNG    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University   ); KIM DONG-JAE    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University   ); KIM CHANG-HWAN    (Toxicology Center, LG Life Sciences   ); PARK JAE-HAK    (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University  );
  • 초록

    We recently isolated a novel probiotic strain, Lactobacillus fermentum PL9005 (KCCM-10250), from infant feces and showed that it had a potential immunoenhancing effect. In the present study, a safety assessment of the bacteria was performed using a BALB/c mouse model. Mice were administered with L. fermentum PL9005 daily for 28 days. There were no detectable changes in body weight, feed intake, or clinical signs, and no significant difference in hematological parameters or blood biochemistry between the L. fermentum PL9005-fed and control groups. Bacterial translocation was detected in the mesenteric lymph nodes, liver, and spleen of some mice with and without L. fermentum PL9005 feeding, however, the organisms were not related to ingestion of L. fermentum PL9005; this was confirmed by PCR using a species-specific primer. No gross lesions were detected in the liver, spleen, or intestine of L. fermentum PL9005-fed or control mice. Mucosal thickness in the ileum, cecum, and colon of L. fermentum PL9005-fed mice was not significantly different from that of corresponding organs in control mice. No inflammation or epithelial cell degeneration in the intestines was observed in any mice. These results indicate that ingestion of L. fermentum PL9005 is safe in mice and can be applied in the functional food market.


  • 주제어

    Lactobacillus fermentum PL9005 .   immunoenhancing effect .   safety assessment .   mice.  

  • 참고문헌 (38)

    1. Aguirre, M. and M. D. Collins. 1993. Lactic acid bacteria and human clinical infection. J. Appl. Bacteriol. 75: 95-107 
    2. Berg, R. D. 1983. Translocation of indigenous bacteria from the intestinal tract, pp. 333-352. In R. J. Hentges (ed.). Human Intestinal Microflora in Health and Disease. Academic Press, London 
    3. Boyd, H. B. 1993. Safety assessment of novel foods. Intern. Food Ingred. 5: 9-11 
    4. Hale, P. and A. Hill. 1973. The recovery of Lactobacillus sp. from the livers of healthy mice. Lab. Anim. 7: 119-124 
    5. Holzapfel, W. H., P. Haberer, J. Snel, U. Schillinger, and J. H. J. Huis in't Veld. 1998. Overview of gut flora and probiotics. Int. J. Food Microbiol. 41: 85-101 
    6. Kim, S. M., K. H. Lee, N. K. Lee, C. J. Kim, C. H. Kim, and H. D. Park. 2004. Antagonistic activity of polyfermenticin SCD against Helicobacter pylori KCTC 2948. J. Microbiol. Biotechnol. 14: 148-152 
    7. Klein, G., A. Pack, C. Bonaparte, and G. Reuter. 1998. Taxonomy and physiology of probiotic lactic acid bacteria. Int. J. Food Microbiol. 41: 103-125 
    8. Willem, M., R. Fonden, U. Svensson, M. Saxelin, L. Morelli, K. Collins, D. Brassard, E. Shiffrin, G. Mogensen, B. Grenov, S. Salminen, E. Isolauri, A. von Wright, and T. M. Sandholm. 1997. Recommendations for safety of probiotic cultures for traditional or novel foods. Demonst. Nutr. Functional. Probio. Food Newslett. 2: 2 
    9. Fukushima, M. and M. Nakano. 1996. Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet. Brit. J. Nutr. 76: 857-867 
    10. Zhou, J. S., Q. Shu, K. J. Rutherfurd, J. Prasad, M. J. Birtles, P. K. Gopal, and H. S. Gill. 2001. Safety assessment of potential probiotic lactic acid bacterial strains Lactobacillus rhamnosus HN001, Lb. acidophilus HN017, and Bifidobacterium lactis HN019 in BALB/c mice. Int. J. Food Microbiol. 56: 87-96 
    11. Ward, L. J. H., J. C. S. Brown, and G. P. Davey. 1995. Detection of dairy Leuconostoc strains using the polymerase chain reaction. Lett. Appl. Microbiol. 20: 204-208 
    12. De Rodas, B. Z., S. E. Gilliland, and C. V. Maxwell. 1996. Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J. Dairy Sci. 79: 2121-2128 
    13. Ford, H. R., A. Avanoglu, P. R. Boechat, R. Melgoza, R. S. LumCheong, P. Boyle, M. Garrett, and M. I. Rowe. 1996. The microenvironment influences the pattern of bacterial translocation in formula-fed neonates. J. Pediat. Surg. 31: 486-489 
    14. Fuller, R. 1991. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378 
    15. Gill, H. S., K. J. Rutherfurd, J. Prasad, and P. K. Gopal. 2000. Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Brit. J. Nutr. 83: 167-176 
    16. Marteau, P. and J. C. Rambaud. 1993. Potential of using lactic acid bacteria for therapy and immunomodulation in man. Microbiol. Rev. 12: 207-220 
    17. Walter, J., G. W. Tannock, A. Tilsala- Timisjarvi, S. Rodtong, D. M. Loach, K. Munro, and T. Alatossava. 2000. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and speciesspecific PCR primers. Appl. Environ. Microbiol. 66: 297-303 
    18. Zhou, J. S., Q. Shu, K. J. Rutherfurd, J. Prasad, P. K. Gopal, and H. S. Gill. 2000. Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem. Toxicol. 38: 153-161 
    19. Park, J. H., Y. Lee, E. Moon, S. H. Seok, S. A. Cho, M. W. Baek, H. Y. Lee, D. J. Kim, and J. H. Park. 2005. Immunoenhancing effects of a new probiotic strain, Lactobaeillus fermentum PL 9005. J. Food. Prot. 68: 571-576 
    20. Fuller, R. 1992. History and development of probiotics, pp. 1-8. In Fuller, R. (ed.), Probiotics - The Scientific Basis. Chapman and Hall, London 
    21. Hull, R. 1995. Probiotics and human health. Proc. Nutr. Soc. Aust. 19: 88-93 
    22. Tortuero, F., J. Rioperez, E. Fernandez, and M. L. Rodriguz. 1995. Response of piglets to oral administration of lactic acid bacteria. J. Food Prot. 58: 1369-1374 
    23. Donohue, D. C., S. Salminen, and P. Marteau. 1998. Safety of probiotic bacteria, pp. 307. In S. Salminen and A. von Wright (eds.) Lactic Acid Bacteria. Marcel Dekker, New York, U.S.A 
    24. Gill, H. S., Q. Shu, H. Lin, K. J. Rutherfurd, and M. L. Cross. 2001. Protection against trans locating Salmonella typhimurium infection in mice by feeding the immunoenhancing probiotic Lactobacillus rhamnosus strain HN001. Med. Microbiol. Immunol. 190: 97-104 
    25. Swendseid, M. E. 1987. Biochemical assessment of protein and amino acid status, pp. 205-211. In Livingston, G. E. (ed.), Proceedings of a National Conference Sponsored by the Food and Nutrition Council of the American Health Foundation, Nutritional Status Assessment of the Individual. Food and Nutrition Press, New York, U.S.A 
    26. Kim, H. J., J. H. Kim, J. H. Son, H. J. Seo, S. J. Park, N. S. Paek, and S. K. Kim. 2004. Characterization of bacteriocin produced by Lactobacillus bulgaricus. J. Microbiol. Biotechnol. 14: 503-508 
    27. Matar, C., J. C. Valdez, M. Medina, M. Rachid, and G. Perdigon. 2001. Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant. J. Dairy Res. 68: 601-609 
    28. Kato, J., K. Endo, and S. Rybka. 1997. Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Int. J. Immunopharmacol. 16: 29-36 
    29. Lee, H. Y., J. H. Park, S. H. Seok, S. A. Cho, M. W. Baek, D. J. Kim, Y. H. Lee, and J. H. Park. 2004. Dietary intake of various lactic-acid bacteria suppresses type 2 helper T cell-production in antigen-primed mice splenocyte. J. Microbiol. Biotechnol. 14: 167-170 
    30. Ma, L., E. Deitch, R. Specian, E. Steffen, and R. Berg. 1990. Translocation of Lactobacillus murinus from the gastrointestinal tract. Curr. Microbiol. 20: 177-184 
    31. Gasser, F. 1994. Safety of lactic acid bacteria and their occurrence in human clinical infections. Bull. Pasteur Instit. 92: 45-67 
    32. Perdigon, G., M. E. N. de Marcias, S. Alvarez, G. Oliver, and A. P. de Ruiz Holgado. 1990. Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J. Dairy Res. 57: 255-264 
    33. Gordon, L. E., D. Ruml, H. J. Hahne, and C. P. Miller. 1995. Studies on susceptibility to infection following radiation. IV: The pathogenesis of endogenous bacteremia of mice. J. Exp. Med. 102: 413-424 
    34. Shu, Q., K. J. Rutherfurd, S. G. Fenwick, J. Prasad, P. K. Gopal, and H. S. Gill. 2000. Dietary Bifidobacterium lactis (HN019) enhances resistance to oral Salmonella typhimurium infection in mice. Microbiol. Immunol. 44: 213-222 
    35. Kim, S. H., S. J. Yang, H. C. Koo, W. K. Bae, J. Y. Kim, J. H. Park, Y. J. Baek, and Y. H. Park. 2001. Inhibitory activity of Bifidobacteria longum HY8001 against vero cytotoxin of Escherichia coli O157:H7. J. Food Prot. 64: 1667-1673 
    36. Bayer, A. S., A. W. Chow, D. Betts, and L. B. Guze. 1978. Lactobacillemia - report of nine cases, and important clinical and therapeutic considerations. Am. J. Med. 64: 808-813 
    37. Matsuzaki, T. 1998. Immunomodulation by treatment with Lactobacillus casei strain Shirota. Int. J. Food Microbiol. 41: 133-140 
    38. van der Kamp, J. W. 1996. Safety considerations regarding probiotic strains - conclusions of two specialist workshops. Int. Dairy Feder. Newslett. 5: 27 - 28 
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    2. 2008. "" Journal of microbiology and biotechnology, 18(2): 338~342     

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