본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.633 - 639   피인용횟수: 5

Therapeutic Effect of Astaxanthin Isolated from Xanthophyllomyces dendrorhous Mutant Against Naproxen-Induced Gastric Antral Ulceration in Rats

KIM JEONG-HWAN    (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University   ); KIM SEUNG-WOOK    (Department of Chemical and Biological Engineering, Korea University   ); YUN CHEOL-WON    (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University   ); CHANG HYO-IHL    (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University  );
  • 초록

    Frequently used for humans as a nonsteroidal anti-inflammatory drug, naproxen has been known to induce ulcerative gastric lesions. The present study was undertaken to investigate the in vivo therapeutic effect of astaxanthin, isolated from a Xanthophyllomyces dendrorhous mutant, against naproxen-induced gastric antral ulceration in rats. The rats were treated with three doses of astaxanthin [1, 5, and 25 mg/kg body weight (B.W.), respectively] once daily for 2 weeks after pretreatment of 80 mg of naproxen/kg B.W. twice daily for 3 days, while the control rats received only 80 mg of naproxen/kg B.W. twice daily for 3 days. The oral administration of astaxanthin (1,5, and 25 mg/kg B.W.) showed a curative effect against naproxen (80 mg/kg B.W.)-induced gastric antral ulcer and reduced the elevated lipid peroxide level in gastric mucosa. In addition, astaxanthin treatment resulted in significant increase in the activities of radical scavenging enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. A histologic examination clearly proved that acute gastric mucosal lesion induced by naproxen nearly disappeared after the astaxanthin treatment. These results suggest that astaxanthin eliminated the lipid peroxides and free radicals induced by naproxen and may be a potential candidate for remedy of gastric ulceration.


  • 주제어

    Anti-ulcer drug .   astaxanthin .   gastric antral ulceration .   orogastric administration .   Xanthophyllomyces dendrorhous.  

  • 참고문헌 (40)

    1. Andrew, A. G., H. J. Phaff, and M. P. Starr. 1976. Carotenoids of Phaffia rhodozyma, a red pigmented fermenting yeast. Phytochemistry 15: 1003 - 1007 
    2. Beck, W. S., H. T. Schneider, K. Dietzel, B. Nuernberg, and K. Brune. 1990. Gastrointestinal ulcerations induced by antiinflammatory drugs in rats. Arch. Toxicol. 64: 210-217 
    3. Bendich, A. and J. A. Olson. 1989. Biological actions of carotenoides. FASEB J. 3: 1927 -1932 
    4. Cioli, V., S. Putzolu, V. Rossi, B. P. Scorza, and C. Corradino. 1979. The role of direct tissue contact in the production of gastrointestinal ulcers by anti-inflammatory drugs in rats. Toxicol. Appl. Pharmacol. 50: 283-289 
    5. Kim, J. H., C. W. Kim, and H. I. Chang. 2004. Screening and characterization of red yeast Xanthophyllomyces dendrorhous mutants. J. Microbiol. Biotechnol. 14: 570-575 
    6. Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358 
    7. Sun, N. K., S. H. Lee, G. H. Ahn, M. S. Won, and K. B. Song. 2002. Low-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant. J. Microbiol. Biotechnol. 12: 1010-1012 
    8. Tanaka, T., H. Makita, M. Ohnishi, H. Mori, K. Satoh, and A. Hara. 1995. Chemoprevention of rat oral carcinogenesis by naturally occurring xanthophylls, astaxanthin and canthaxanthin. Cancer Res. 55: 4059-4064 
    9. Tenenbaum, J. 1999. The epidemiology of nonsteroidal anti-inflammatory drugs. Can. J. Gastroenterol. 13: 119-122 
    10. Parks, D. A. 1989. Oxygen radicals: Mediators of gastrointestinal pathophysiology. Gut 30: 293-298 
    11. Tanaka, T., T. Kawamori, M. Ohnishi, H. Makita, H. Mori, K. Satoh, and A. Hara. 1995. Suppression of azoxymethane-induced rat colon carcinogenesis by dietary administration of naturally occurring xanthophylls, astaxanthin and canthaxanthin, during the postinitiation phase. Carcinogenesis 16: 2957-2963 
    12. Jyonouchi, H., L. Zhang, M. Gross, and Y. Tomita. 1994. Immunomodulating actions of carotenoids: Enhancement of in vivo and in vitro antibody production to T-dependent antigens. Nutr. Cancer 21: 47-58 
    13. McCarthy, D. M. 1990. NSAID-induced gastro-intestinal damage - a critical review of prophylaxis and therapy. J. Clin. Gastroenterol. 12: S13-S20 
    14. Tanaka, T., Y. Morishita, M. Suzuki, T. Kojima, A. Okumura, and H. Mori. 1994. Chemoprevention of mouse urinary bladder carcinogenesis by the naturally occurring carotenoid astaxanthin. Carcinogenesis 15: 15-19 
    15. Lim, B. P., A. Nagao, J. Terao, K. Tanaka, T. Suzuki, and K. Takama. 1992. Antioxidant activity of xanthophylls on peroxyl radical-mediated phospholipid peroxidation. Biochim. Biophys. Acta 1126: 178-184 
    16. Sedmak, J. J., D. K. Weerasinghe, and S. O. Jolly. 1990. Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotech. Tech. 4: 107-112 
    17. Lanza, F. L. 1984. Endoscopic studies of gastric and duodenal injury after the use of ibuprofen, aspirin, and other nonsteroidal anti-inflammatory agents. Am. J. Med. 13: 1924 
    18. McCarthy, D. M. 1995. Mechanisms of mucosal injury and healing: The role of nonsteroidal anti-inflammatory drugs. Scand. J. Gastroenterol. 208: 24-29 
    19. Johnson, E. A. and M. J. Lewis. 1979. Astaxanthin formation by the yeast Phaffia rhodozyma. J. Gen. Microbiol. 115: 173-183 
    20. Kim, J. H., S. K. Choi, W. J. Lim, and H. I. Chang. 2004. Protective effect of astaxanthin produced by Xanthophyllomyces dendrorhous mutant on indomethacin-induced gastric mucosal injury in rats. J. Microbiol. Biatechnol. 14: 996-1003 
    21. Krinsky, N. I. 1989. Antioxidant function of carotenoids. Free Radic. Biol. Med. 7: 617-635 
    22. Aebi, H. 1974. In H. U. Bergmeyer (ed.). Methods of Enzymatic Analysis. pp. 674-678. Academic Press, New York, U.S.A 
    23. Yoshikawa, T., Y. Naito, S. Ueda, H. Oyamada, T. Takemura, N. Yoshida, S. Sugino, and M. Kondo. 1990. Role of oxygen-derived free radicals in the pathogenesis of gastric mucosal lesions in rats. J. Clin. Gastroenterol. 12: 65-71 
    24. Naguib, Y. M. 2000. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food Chem. 48: 1150-1154 
    25. Lewis, M. J., N. Ragot, M. C. Berlant, and M. Miranda. 1990. Selection of astaxanthin-overproducing mutants of Phaffia rhodozyma with ${\beta}$-ionone. Appl. Environ. Microbiol. 56: 2944-2945 
    26. Palozza, P. and N. I. Krinsky. 1992. Astaxanthin and canthaxanthin are potent antioxidants in a membrane model. Arch. Biochem. Biophys. 297: 291-295 
    27. Kim, S. J., G. J. Kim, D. H. Park, and Y. W. Ryu. 2003. High-level production of astaxanthin by fed-batch culture of mutant strain Phaffia rhodozyma AJ-6-1. J. Microbiol. Biotechnol. 13: 175-181 
    28. Roth, S. H. and R. E. Bennett. 1987. Non-steroidal anti-inflammatory drug gastropathy: Recognition and response. Arch. Intern. Med. 147: 2093-2100 
    29. Jyonouchi, H., S. Sun, K. Lijima, and M. D. Gross. 2000. Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer 36: 59-65 
    30. Kurashige, M., E. Okimasu, M. Inoue, and K. Utsumi. 1990. Inhibition of oxidative injury of biological membranes by astaxanthin. Physiol. Chem. Physics Med. NMR 22: 27-38 
    31. Okai, Y. and K. Higashi-Okai. 1996. Possible immunomodulating activities of carotenoids in in vitro cell culture experiments. Int. J. Immunopharmacol. 18: 753-758 
    32. An, G. H., D. B. Schuman, and E. A. Johnson. 1989. Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl. Environ. Microbiol. 55: 116-124 
    33. Calhoun, W., S. C. Gilman, L. J. Datko, T. W. Copenhaver, and R. P. Carlson. 1992. Interaction studies of tilomisole, aspirin and naproxen in acute and chronic inflammation with assessment of gastrointestinal irritancy in the rat. Agents Actions 36: 99-106 
    34. Hogeboom, G. H. 1955. In S. P. Colowick and N. O. Kaplan (eds.). Methods in Enzymology, pp. 16-19. Academic Press, New York, U.S.A 
    35. McCord, J. M. and J. Fridovich. 1967. Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6055 
    36. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
    37. Bjarnason, I., J. Hayllar, A. J. MacPherson, and A. Russell. 1993. Side effects of nonsteroidal anti-inflammatory drugs on the small and large intestine in humans. Gastroenterology 104: 1832-1847 
    38. Lawrence, R. A. and R. F. Burk. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71: 952-958 
    39. Mortensen, A., L. H. Skibsted, and T. G. Truscott. 2001. The interaction of dietary carotenoids with radical species. Arch. Biochem. Biophys. 385: 13 - 19 
    40. Suwa, T., H. Urano, Y. Kohno, A. Suzuki, and T. Amano. 1987. Comparative studies on the gastrointestinal lesions caused by several nonsteroidal anti-inflammatory agents in rats. Agents Actions 21: 167-172 
  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" Journal of microbiology and biotechnology, 16(12): 1890~1896     
    2. 2006. "" Journal of microbiology and biotechnology, 16(3): 438~442     
    3. 2006. "" Journal of microbiology and biotechnology, 16(6): 821~831     
    4. 2006. "" Journal of microbiology and biotechnology, 16(8): 1222~1228     
    5. 2007. "" Journal of microbiology and biotechnology, 17(9): 1573~1578     

 저자의 다른 논문

  • 김정환 (4)

    1. 2004 "Screening and Characterization of Red Yeast Xanthophyllomyces dendrorhous Mutants" Journal of microbiology and biotechnology 14 (3): 570~575    
    2. 2004 "Protective Effect of Astaxanthin Produced by Xanthophyllomyces dendrorhous Mutant on Indomethacin-Induced Gastric Mucosal Injury in Rats" Journal of microbiology and biotechnology 14 (5): 996~1003    
    3. 2006 "High-Level Production of Astaxanthin by Xanthophyllomyces dendrorhous Mutant JH1, Using Chemical and Light Induction" Journal of microbiology and biotechnology 16 (3): 381~385    
    4. 2006 "Effect of Culture Conditions on Astaxanthin Formation in Red Yeast Xanthophyllomyces dendrorhous Mutant JH1" Journal of microbiology and biotechnology 16 (3): 438~442    
  • Kim, Seung-Wook (64)

  • Yun, Cheol-Won (8)

  • Chang, Hyo-Ihl (23)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기