본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.640 - 645   피인용횟수: 5

The Role of a Second Protein (Des VIII) in Glycosylation for the Biosynthesis of Hybrid Macrolide Antibiotics in Streptomyces venezuelae

HONG JAY SUNG JOONG    (Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University   ); KIM WON SEOK    (Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University   ); LEE SANG KIL    (School of Chemical and Biological Engineering, College of Engineering, Seoul National University   ); KOH HWA SOO    (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University   ); PARK HEE SUB    (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University   ); PARK SU JIN    (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University   ); KIM YOUN SANG    (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University   ); YOON YEO JOON    (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University  );
  • 초록

    The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose.


  • 주제어

    Glycosylation .   Streptomyces venezuelae .   deoxysugar.  

  • 참고문헌 (23)

    1. Aguirrezabalaga, J., C. Olano, N. Allende, L. Rodriguez, A. F. Brana, C. Mendez, and J. A. Salas. 2000. Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus. Antimicrob. Agents Chemother. 44: 1266-1275 
    2. Kim, C. Y., H. J. Park, and E. S. Kim. 2003. Heterologous expression of hybrid type 2 polyketide synthase system in Streptomyces species. J. Microbiol. Biotechnol. 13: 819-822 
    3. Kim, W. G. 2002. Benzastatin J, a new demethylated derivative of benzastatin B produced by controlled fermentation of Streptomyces nitrosporeus. J. Microbiol. Biotechnol. 12: 838-840 
    4. Xue, Y., L. Zhao, H. W. Liu, and D. H. Sherman. 1998. A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity. Proc. Natl. Acad. Sci. USA 95: 12111-12116 
    5. Xue, Q., G. Ashley, C. R. Hutchinson, and D. V. Santi. 1999. A multi-plasmid approach to preparing large libraries of polyketides. Proc. Natl. Acad. Sci. USA 96: 11740-11745 
    6. Sohng, J. K., H. C. Lee, K. Liou, E. B. Lee, S.-Y. Kang, and J.-S. Woo. 2003. Cystocin, a novel antibiotic, produced by Streptomyces sp. GCA0001 : Production and characterization of cystocin. J. Microbiol. Biotechnol. 13: 483-486 
    7. Jung, W. S., E. S. Kim, H. Y. Kang, C. Y. Choi, D. H. Sherman, and Y. J. Yoon. 2003. Site-directed mutagenesis on putative macrolactone ring size determinant in the hybrid pikromycin-tylosin polyketide synthase. J. Microbiol. Biotechnol. 13: 823-828 
    8. Summers, R. G., S. Donadio, M. J. Staver, E. Wendt-Pienkowski, C. R. Hutchinson, and L. Katz. 1997. Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythrea that are involved in L-mycarose and D-desosamine production. Microbiology 143: 3251-3262 
    9. Borisova, S. A., L. Zhao, D. H. Sherman, and H. W. Liu. 1999. Biosynthesis of desosamine: Construction of a new macrolide carrying a genetically designed sugar moiety. Org. Lett. 1: 133-136 
    10. Hoffmeister, D., K. S. lchinose, B. Domann, A. Faust, G. Trefzer, A. Drager, C. Kirschning, E. Fischer, D. Kunzel, J. Bearden, J. Rohr, and A. Bechthold. 2000. The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: Cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. Chem. Biol. 7: 821-831 
    11. McDaniel, R., A. Thamchaipenet, C. Gustafsson, H. Fu, M. Betlach, and G. Ashley. 1999. Multiple genetic modifications of the erythromycin gene cluster to produce a library of novel unnatural natural products. Proc. Natl. Acad. Sci. USA 96: 1846-1851 
    12. Zhao, L., D. H. Sherman, and H. W. Liu. 1998. Biosynthesis of desosamine: Construction of a new methymycin/neomethymycin analogue by deletion of a desosamine biosynthetic gene. J. Am. Chem. Soc. 120: 10256-10257 
    13. Zhao, L., S. Borisova, S. M. Yeung, and H. W. Liu. 2001. Study of C-4 deoxygenation in the biosynthesis of desosamine: Evidence implicating a novel mechanism. J. Am. Chem. Soc. 123: 7909-7910 
    14. Rodriguez, L., I. Aguirrezabalaga, N. Allende, F. Brana, C. Mendez, and J. A. Salas. 2002. Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms: A tool to produce novel glycosylated bioactive compounds. Chem. Biol. 9: 721-729 
    15. Otten, S. L., X. Liu, J. Ferguson, and C. R. Hutchinson. 1995. Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyltransferase involved in daunorubicin biosynthesis. J. Bacteriol. 177: 6688-6692 
    16. Weymouth-Wilson, A. C. 1997. The role of carbohydrates in biologically active natural products. Nat. Prod. Rep. 14: 99-100 
    17. Vara, J., M. Lewandowska-Skarbek, Y. G. Wang, S. Donadio, and C. R. Hutchinson. 1989. Cloning of genes governing the deoxysugar portion ofthe erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J. Bacteriol. 171: 5872-5881 
    18. Zhao, L., N. J. Beyer, S. A. Borisova, and H. W. Liu. 2003. ${\beta}$-glucosylation as a part of self-resistance mechanism in methymycin/pikromycin producing strain Streptomyces venezuelae. Biochemistry 42: 14794-14804 
    19. Borisova, S. A., L. Zhao, C. E. Melancon III, C. L. Kao, and H. W. Liu. 2004. Characterization of the glycosyltransferase activity of des VII: Analysis of and implications for the biosynthesis of macrolide antibiotics. J. Am. Chem. Soc. 126: 6534-6535 
    20. Raty, K., T. Kunnari, J. Hakala, P. Mantsala, and K. Ylihonko. 2000. A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. Mol. Gen. Genet. 264: 164-172 
    21. Keiser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces Genetics. Norwich, The John Innes Foundation 
    22. Zhao, L., N. L. S. Que, Y. Xue, D. H. Sherman, and H. W. Liu. 1998. Mechanistic studies of desosamine biosynthesis: C-4 deoxygenation precedes C-3 transamination. J. Am. Chem. Soc. 120: 12159-12160 
    23. Ward, J. M., G. R. Janssen, T. Kieser, M. J. Bibb, and M. J. Buttner. 1986. Construction and characterization of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol. Gen. Genet. 203: 468-478 
  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" Journal of microbiology and biotechnology, 16(5): 667~672     
    2. 2006. "" Journal of microbiology and biotechnology, 16(6): 974~978     
    3. 2007. "" Journal of microbiology and biotechnology, 17(12): 1909~1921     
    4. 2007. "" Journal of microbiology and biotechnology, 17(3): 539~542     
    5. 2008. "" Journal of microbiology and biotechnology, 18(5): 866~873     

 저자의 다른 논문

  • HONG JAY SUNG JOONG (1)

    1. 2003 "Premature Release of Polyketide Intermediates by Hybrid Polyketide Synthase in Amycolatopsis mediterranei S699" Journal of microbiology and biotechnology 13 (4): 613~619    
  • 이상길 (3)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기