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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.672 - 677   피인용횟수: 1

Diversity and Genotypic Structure of ECOR Collection Determined by Repetitive Extragenic Palindromic PCR Genome Fingerprinting

HWANG KEUM-OK    (Department of Environmental Sciences, Hankuk University of Foreign Studies   ); JANG HYO-MI    (Department of Environmental Sciences, Hankuk University of Foreign Studies   ); CHO JAE-CHANG    (Department of Environmental Sciences, Hankuk University of Foreign Studies  );
  • 초록

    The standard reference collection of strains for E. coli, the ECOR collection, was analyzed by a genome-based typing method. Seventy-one ECOR strains were subjected to repetitive extragenic palindromic PCR genome fingerprinting with BOX primers (BOX-PCR). Using a similarity value of 0.8 or more after cluster analysis of BOX-PCR fingerprinting patterns to define the same genotypes, we identified 28 genotypes in the ECOR collection. Shannon's entropy-based diversity index was 3.07, and the incident-based coverage estimator indicated potentially 420 genotypes among E. coli populations. Chi-square test of goodness-of-fit showed statistically significant association between the genotypes defined by BOX-PCR fingerprinting and the groups previously defined by multi-locus enzyme electrophoresis. This study suggests that the diversification of E. coli strains in natural populations is actively ongoing, and rep-PCR fingerprinting is a convenient and reliable method to type E. coli strains for the purposes ranging from ecology to quarantine.ine.


  • 주제어

    Genotype .   diversity .   ECOR .   BOX-PCR.  

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    1. 2006. "" Journal of microbiology and biotechnology, 16(11): 1773~1777     

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