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Journal of microbiology and biotechnology v.15 no.3, 2005년, pp.678 - 682   피인용횟수: 2

Efficient Expression of a Carbon Starvation Promoter Activity Under Nutrient-Limited Chemostat Culture

KIM DAE-SUN    (Division of Biotechnology, Catholic University of Korea   ); PARK YONG-IL    (Division of Biotechnology, Catholic University of Korea   ); LEE HYANG BURM    (School of Biological Sciences, Seoul National University   ); KIM YOUNGJUN    (Division of Biotechnology, Catholic University of Korea  );
  • 초록

    The promoter region of a carbon starvation gene isolated from Pseudomonas putida was cloned and analyzed for its potential use for in situ bioremediation and bioprocessing. We constructed a recombinant plasmid pMKD101 by cloning the 0.65 kb promoter region of the gene into the promoter proving vector, pMK301, which contains the lacZ for ${\beta}$ -galactosidase activity as a reporter gene. pMKD101 was transformed into the wild-type P. putida MK1, resulting in P. putida RPD101, and analyzed for ${\beta}$ -galactosidase activity under different culture conditions. When RPD101 was grown on the minimal medium plus $0.1\%$ glucose as a sole carbon source in batch cultures, ${\beta}$ -galactosidase activity was found to be 3.2-fold higher during the stationary phase than during the exponential phase. In chemostat cultures, ${\beta}$ -galactosidase activity was found to be 3.1-fold higher at the minimal growth rate (dilution rate= $0.05\;h^{-1}$ ) than at the maximal growth rate (dilution rate= $0.173;h^{-1}$ ). The results suggest that a carbon starvation promoter can be utilized to maximize the expression of a desired gene under nutrient limitation.


  • 주제어

    Starvation promoter .   bioremediation .   bioprocessing .   Pseudomonas putida.  

  • 참고문헌 (17)

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  • 이 논문을 인용한 문헌 (2)

    1. 2007. "" Journal of microbiology and biotechnology, 17(9): 1452~1459     
    2. 2007. "" Journal of microbiology and biotechnology, 17(2): 373~377     

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