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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.683 - 688   피인용횟수: 3

An Efficient and Stable Method for the Transformation of Heterogeneous Genes into Cephalosporium acremonium Mediated by Agrobacterium tumefaciens

XU WEI    (Department of Pharmaceutical Engineering, Shenyang Pharmaceutical University   ); ZHU CHUNBAO    (Department of Biopharmacevtics, Shanghai Institute of Pharmaceutical Industry(SIPI)   ); ZHU BAOQUAN    (Department of Biopharmacevtics, Shanghai Institute of Pharmaceutical Industry(SIPI)  );
  • 초록

    A transformation system mediated by Agrobacterium tumefaciens is routinely used for the genetic engineering of plants. Here, we report an efficient and stable method for transformation of heterogeneous genes into an industrial Cephalosporium acremonium by using a similar transformation system established in plants. Both the phleomycin-resistant gene and vgb gene were used as screening markers to confirm the success of transformation by either Southern hybridization or PCR amplification. It was found that acetosyringone (AS) was necessary only for protoplast transformation and the heterogeneous genes transferred were integrated into the genome of C. acremonium. The transformation efficiency obtained with this system was much higher than the conventional techniques used for transformation of C. acremonium.


  • 주제어

    Agrobacterium tumefaciens .   Cephalosporium acremonium .   protoplast .   Ti plasmid .   transformation .   triparental mating .   vgb.  

  • 참고문헌 (16)

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    14. Raeder, U. and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1: 17-20 
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  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of microbiology and biotechnology, 16(8): 1236~1239     
    2. 2007. "" Journal of microbiology and biotechnology, 17(4): 547~559     
    3. 2008. "" Journal of microbiology and biotechnology, 18(2): 234~241     

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