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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.734 - 739   피인용횟수: 3

Purification and Characterization of CDMHK, a Growth Inhibitory Molecule Against Cancer Cell Lines, from Myxobacterium sp. HK1 Isolated from Korean Soil

LEE HAN-KI    (Division of Bioscience and Bioinformatics, Myongji University   ); LEE IN-HYE    (Division of Bioscience and Bioinformatics, Myongji University   ); YIM JEE-SUN    (Division of Bioscience and Bioinformatics, Myongji University   ); KIM YONG-HO    (Division of Bioscience and Bioinformatics, Myongji University   ); LEE SANG-HEE    (Division of Bioscience and Bioinformatics, Myongji University   ); LEE KISAY    (Department of Environmental Engineering and Biotechnology, Myongji University   ); KOO YOON-MO    (Department of Biological Engineering, Inha University   ); KIM SANG-JIN    (Microbiology Laboratory, Korea Ocean Research and Development Institute   ); JEONG BYEONG-CHUL    (Division of Bioscience and Bioinformatics, Myongji University  );
  • 초록

    Myxobacterium sp. HK1, isolated from Korean soil, degrades cellulose, differentiates to fruiting body, and its 16s rDNA has $95\%$ similarity to Polyangium sp. An anticancer molecule, CDMHK, was identified from culture broth of Myxobacterium sp. HK1, and purified by Diaion HP20, Silica gel, Sephadex LH-20 chromatography, and preparative HPLC using an YMC OSD-A C18 column. The molecular structure and formula were determined to be $C_{l2}H_{l9}N_3O_2$ (M.W 237) by MS spectrometry, 300 MHz $^{1}H\;and\;^{13}C$ NMR. The CDMHK was not active against Escherichia coli, Staphylococcus aureus, and Candida albicans. However, this molecule inhibited the growth of various cancer cell lines. The $ED_{50}$ values of CDMHK were determined to be 0.147, 0.086, 0.18, 0.166, and 0.142 $\mu$ g/ml against A549, SK-OV-3, SK-MEL-2, VF498, and HCTl5 cancer cell lines, respectively. In addition, the CDMHK was able to induce apoptosis of the CCRF-CEM cancer cell line, evidenced by DNA fragmentation assay and DAPI staining.


  • 주제어

    Myxobacterium sp.   HK1 .   growth inhibitor .   cancer cell lines .   apoptosis.  

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  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of microbiology and biotechnology, 16(8): 1262~1268     
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