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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.767 - 771   피인용횟수: 7

Expression of Hepatitis C Virus Structural Proteins in Saccharomyces cerevisiae

LEE JONG-SOO    (BioLeaders Corporation   ); YU JUNG    (Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University   ); SHIN HYUN-JIN    (BioLeaders Corporation   ); KIM YOUNG-SANG    (Department of Biochemistry, Chungnam National University   ); AHN JEONG-KEUN    (Microbiology, College of Natural Sciences, Chungnam National University   ); LEE CHONG-KIL    (Department of Pharmacy, Chungbuk National University   ); POO HARYOUNG    (Proteome Research Lab., Korea Research Institute of Bioscience and Biotechnology   ); KIM CHUL-JOONG    (Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University  );
  • 초록

    Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/ $cm^3$ . These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.


  • 주제어

    HCV structural proteins .   yeast expression .   vaccine.  

  • 참고문헌 (20)

    1. Chan-Fook, C., W. R. Jiang, B. E. Clarke, N. Zitzmann, C. Maidens, J. A. McKeating, and J. M. Jones. 2000. Hepatitis C virus glycoprotein E2 binding to CD81: The role of E 1 E2 cleavage and protein glycosylation in bioactivity. Virology 273: 60-66 
    2. Charloteaux, B., L. Lins, H. Moereels, and R. Brasseur. 2002. Analysis of the C-terrninal membrane anchor domains of hepatitis C virus glycoproteins EI and E2: Toward a topological model. J. Virol. 76: 1944-1958 
    3. Choukhi, A., A. Pillez, H. Drobecq, C. Sergheraert, C. Wychowski, and J. Dubuisson. 1999. Characterization of aggregates of hepatitis C virus glycoproteins. J. Gen. Virol. 80: 3099-3107 
    4. Falcon, V., C. Garcia, M. C. Rosa, I. Menendez, J. Seoane, and J. M. Grillo. 1999. Ultrastructural and immunocytochemical evidences of core-particle formation in the methylotrophic Pichia pastoris yeast when expressing HCY structural proteins (core-El). Tissue Cell 31: 117-125 
    5. Hinnen, A., J. B. Hicks, and G R. Fink. 1992. Transformation of yeast. Biotechnology 24: 337-341 
    6. Yasui, K., T. Wakita, K. Tsukiyama-Kohara, S. Funahashi, M. Ichikawa, T. Kajita, D. Moradpour, J. R. Wands, and M. Kohara. 1998. The native form and maturation process of hepatitis C virus core protein. J. Virol. 72: 6048-6055 
    7. Matatsumoto, M., S. B. Hwang, K. S. Jeng, N. Zhu, and M. N. Lai. 1996. Homotypic interaction and multimerization of hepatitis C virus core protein. Virology 218: 43-51 
    8. Clayton, R. F., A. Owsianka, J. Aitken, S. Graham, D. Bhella, and A. H. Patel. 2002. Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C viruslike particles. J. Virol. 76: 7672- 7682 
    9. Santolini, E., G Migliaccio, and N. Monica 1994. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J. Virol. 68: 3631-3641 
    10. Dubuisson, J. 1999. Folding, assembly and subcellular localization of HCY glcoproteins. Curr. Top. Microbiol. Immunol. 242: 135-148 
    11. Kuroda, S., T. Otaka, T. Miyazaki, M. Nakao, and Y. Fujisawa. 1992. Hepatitis B virus envelope L protein particles. J. Biol. Chem. 267: 1953-1961 
    12. Acosta-Rivero, N., J. C. Alvarez-Obregon, A. Musacchio, V. Falcon, S. Duenas-Carrera, J. Marante, J. Menendez, and J. Morales. 2002. In vitro self-assembly HCV core virus-like particles induce a strong antibody immune response in sheep. Biochem. Biophys. Res. Commun. 290: 300-304 
    13. Bartenschlager, R. and V. Lohmann. 2000. Replication of hepatitis C virus. J. Gen. Virol. 81: 1631- 1648 
    14. Kang, H. A., M. S. Jung, W. K. Hong, J. H. Sohn, E. S. Choi, and S. K. Rhee. 1998. Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisae. J. Microbiol. Biotechnol. 8: 42-48 
    15. Shin, D. J., J. C. Park, H. B. Lee, S. B. Chen, and S. Bai. 1998. Construction of a secretory expression vector producing an ${\alpha}$-amylase of yeast, Schwanniomyces occidentalis in Saccharomyces. J. Microbiol. Biotechnol. 8: 625-630 
    16. Op De Beeck, A., L. Cocquerel, and J. Dubuisson. 2001. Biogenesis of hepatitis C virus envelope glycoproteins. J. Gen. Virol. 82: 2589-2595 
    17. Reed, K. E. and C. M. Rice. 2002. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol. 242: 55-84 
    18. Han, B. K., B. Y. Lee, M. K. Min, and K. H. Jung. 1998. Expression and characterization of recombinant E2 protein of hepatitis C virus by insect cellibaculovirus expression system. J. Microbiol. Biotechnol. 8: 361-368 
    19. Baumert, T. F., J. Vergalla, J. Satoi, M. Thomson, M. Lechmann, D. Herion, H. B. Greenberg, S. Ito, and T. J. Lian. 1999. Hepatitis C virus-like particles synthesized in insect cells as a potential vaccine candidate. Gastroenterology 117: 1397-1407 
    20. Grakoui, A., C. Wychowski, C. Lin, S. M. Feinstone, and C. M. Rice. 1993. Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67: 1385-1395 
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