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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.772 - 779   피인용횟수: 5

Enrichment of Ammonia-Oxidizing Bacteria for Efficient Nitrification of Wastewater

KIM WON-KYOUNG    (Department of Environmental Engineering and Biotechnology, Myongji University   ); CUI RONG    (Department of Environmental Engineering and Biotechnology, Myongji University   ); JAHNG DEOKJIN    (Department of Environmental Engineering and Biotechnology, Myongji University  );
  • 초록

    Ammonia-oxidizing bacteria (AOB) were enriched by repeating fed-batch cultivations in an AOB-selective medium of activated sludges from a domestic wastewater treatment plant. Enriched culture showed strong capabilities of ammonia oxidation [0.810 mg $NH_4^+$ -N/mg mixed liquor suspended solids (MLSS) $\cdot$ day] as well as $NO_x^-$ -N production (0.617 mg $NO_x^-$ -N/ mg MLSS $\cdot$ day). Degree of enrichment was examined through fluorescent in situ hybridization (FISH) analyses using an AOB-specific Cy3-labeled oligonucleotide probe (NSOl90) and terminal-restriction fragment length polymorphism (T-RFLP) analyses. FISH analyses confirmed that the fraction of AOB among 4',6-diamidino-2-phenylindole (DAPI)-stained cells increased from about less than $0.001\%$ to approximately $42\%$ after enrichment of AOB, and T-RFLP analyses showed that bacterial community became simpler as enrichment was continued. When the enriched culture of AOB was added (150 mg/l as dry suspended solid) to the normal activated sludge (3,000 mg/l as dry suspended solid), nitrification efficiencies were improved from 0.020 mg $NO_x^-$ -N/mg MLSS $\cdot$ day to 0.041 mg $NO_x^-$ -N/mg MLSS $\cdot$ day in a synthetic wastewater and also from 0.0007 mg $NO_x^-$ -N/mg MLSS $\cdot$ day to 0.0918 mg $NO_x^-$ -N/mg MLSS $\cdot$ day in a real domestic wastewater. Therefore, it is expected that this enrichment method could be used for improving efficiency of nitrification in wastewater treatment plants.


  • 주제어

    Enrichment .   AOB .   nitrification .   FISH .   T-RFLP.  

  • 참고문헌 (35)

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