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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.831 - 837   피인용횟수: 5

Cold Shock Response of Leuconostoc mesenteroides SY1 Isolated from Kimchi

KIM JONG HWAN    (Division of Applied Life Science, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University   ); PARK JAE-YONG    (Division of Applied Life Science, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University   ); JEONG SEON-JU    (Division of Applied Life Science, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University   ); CHUN JIYEON    (Division of Applied Life Science, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University   ); KIM JEONG HWAN    (Division of Applied Life Science, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University  );
  • 초록

    Low-temperature adaptation and cryoprotection were studied in Leuconostoc mesenteroides SYl, a strain isolated from Kimchi. L. mesenteroides SY1 cells grown in exponential growth phase at $30^{\circ}C$ were exposed to $15^{\circ}C,\;10^{\circ}C$ , and $5^{\circ}C$ for 2, 4, and 6 h, respectively, and then frozen at $- 70^{\circ}C$ for 24 h. Survival ratio was measured after the cells were thawed. The freezing-thawing cycles were repeated four times. Preadapted cells survived better than non-adapted control cells, and the highest survival ratio ( $96\%$ ) was observed for cells preadapted for 2 h at $5^{\circ}C$ , whereas control cells showed only $22\%$ . The 2D gel showed that two proteins (spots A and B) were induced in cells preadapted at lower temperatures. Spots A and B have the same molecular weight (7 kDa), but the pI was 4.6 for spot A and 4.3 for spot B. The first 29 and 15 amino acid sequences from spots A and B were determined, and they were identical, except for one amino acid. A csp gene was cloned, and nucleotide sequencing confirmed that the gene encoded spot A cold shock protein.


  • 주제어

    Cold shock .   Leuconostoc mesenteroides .   Kimchi .   cold shock proteins .   adaptation.  

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  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" Journal of microbiology and biotechnology, 16(6): 983~987     
    2. 2006. "" Journal of microbiology and biotechnology, 16(6): 988~992     
    3. 2007. "" Journal of microbiology and biotechnology, 17(8): 1353~1360     
    4. Jin, Hyo-Sang ; Kim, Jong-Bum ; Yun, Yeong-Ju ; Lee, Kyung-Ja 2008. "Selection of Kimchi Starters Based on the Microbial Composition of Kimchi and Their Effects" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 37(5): 671~675     
    5. 2008. "" Journal of microbiology and biotechnology, 18(4): 746~753     

 저자의 다른 논문

  • Kim, Jong-Hwan (5)

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    5. 2006 "Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi" Journal of microbiology and biotechnology 16 (1): 149~152    
  • Park, Jae-Yong (11)

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