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Isolation and Characterization of Brain-Derived Neurotrophic Factor Gene from Flounder (Paralichthys olivaceus)

LEE JAE HYUNG    (Department of Microbiology, Pukyong National University   ); CHOI TAE-JIN    (Department of Microbiology, Pukyong National University   ); NAM SOO WAN    (Department of Biotechnology and Bioengineering, Dong-Eui University   ); KIM YOUNG TAE    (Department of Microbiology, Pukyong National University  );
  • 초록

    Brain-derived neurotrophic factor (BDNF) is a small secretory protein and a member of the nerve growth factor (NGF) gene family. We cloned the flounder BDNF gene from a flounder brain cDNA library. The nucleotide sequence of the cloned gene showed an open reading frame (ORF) consisting of 810 bp, corresponding to 269 amino acid residues. The tissue distribution of flounder BDNF was determined by reverse transcription-polymerase chain reaction (RT-PCR) in brain, embryo, and muscle tissues. To express fBDNF using a eukaryotic expression system, we constructed the vector mpCTV-BDNF containing the fBDNF gene and transformed this vector into Chlorella ellipsoidea. Stable integration of introduced DNA was confirmed by PCR analysis of genomic DNA, and mRNA expression in C. ellipsoidae was confirmed by RT-PCR analysis.

  • 주제어

    Brain-derived neurotrophic factor .   flounder .   expression .   RT-PCR.  

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