본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Helicobacter pylori Strain 51 (Korean Isolate): Ordered Overlapping BAC Library, Combined Physical and Genetic Map, and Comparative Analysis with H. pylori Strain 26695 and Strain J99

KANG HYUNG-LYUN    (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine   ); LEE WOO-KON    (Department of Microbiology, Gyeongsang National University College of Medicine, Research Institute of Life Science and Central Laboratory, Gyeongsang National University   ); SONG JAE-YOUNG    (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine   ); CHOI SANG-HAENG    (Korea Research Institute for Bioscience and Biotechnology   ); PARK SEONG-GYU    (Department of Microbiology, Gyeongsang National University College of Medicine   ); RYU BOK-DEOK    (Department of Microbiology, Gyeongsang National University College of Medicine   ); LEE EUN-JOO    (Department of Microbiology, Gyeongsang National University College of Medicine   ); KIM JI-SUN    (Korea Research Institute for Bioscience and Biotechnology   ); PARK JEONG-UCK    (Department of Microbiology, Gyeongsang National University College of Medicine, Departmen  ); BAIK SEUNG-CHUL   CHOI MYOUNG-BUM   YOUN HEE-SHANG   KO GYUNG-HYUCK  
  • 초록

    We constructed a defined physical and genetic map of H. pylori strain 51, previously isolated from a Korean patient with a duodenal ulcer, by combining a restriction analysis by pulse-field gel electrophoresis with the construction of a BAC library. A Notl-digest of H. pylori strain 51 genome yielded seven fragments, from which the genomic size was estimated to be 1,698 $\pm$ 24 kb. The BAC library was constructed from 50 to 200 kb fragments of HindIII-digested genomic DNA. From 700 BAC clones, an ordered overlapping maxi-set of 82 BAC clones was assembled that covered the entire genome. The positions of 15 genes were localized in the strain 51 genome with 4-22 kb of resolution and were compared with their orthologues in strain 26695 and strain J99. The arrangement of the 15 genes was identical in strain 51 and strain J99, except for flaA and hpaA. The plasticity zone of strain 51, like that of strain J99, was located in the single region, and was shorter than those of strain 26695 and strain J99. The strain 51 plasticity zone consisted of ORFs common only to strain 51 and J99 or to strain 51 and 26695, as well as strain 51-specific ORFs. Three genetic translocations and/or inversions were found between orthologue ORFs in strain 51 and strain J99. These results show that the chromosomal organization of strain 51 differs from Western strains such as strain 26695 and strain J99.


  • 주제어

    Helicobacter pylori .   genomics .   physical map.  

  • 참고문헌 (47)

    1. Anonymous. 1994. Schistosomes, liver flukes and Helicobacter pylori. IARC Monogr. Eval. Carcinog. Risks Hum. 61: 1241 
    2. Choi, K. M., J. K. Park, and S. Y. Hwang. 2003. Pathogenetic impact of vacuolar degeneration by accelerated transport of Helicobacter pylori VacA. J. Microbiol. Biotechnol. 13: 666-672 
    3. Falush, D., C. Kraft, N. S. Taylor, P. Correa, J. G. Fox, M. Achtman, and S. Suerbaum. 2001 Recombination and mutation during long-term gastric colonization by Helicobacter pylori: Estimates of clock rates, recombination size, and minimal age. Proc. Natl. Acad. Sci. USA 98: 15056-15061 
    4. Jiang, Q., K. Hiratsuka, and D. E. Taylor. 1996. Variability of gene order in different Helicobacter pylori strains contributes to genome diversity. Mol. Microbiol. 20: 833-842 
    5. Jungblut, P. R., D. Bumann, G. Haas, U. Zimny-Arndt, P. Holland, S. Lamer, F. Siejak, A. Aebischer, and T. F. Meyer. 2000. Comparative proteome analysis of Helicobacter pylori. Mol. Microbiol. 36: 710-725 
    6. Kim, J. M., J. E. Shin, M. J. Han, S. H. Park, and D. H. Kim. 2003. Inhibitory effect of Ginseng saponins and polysaccharides on infection and vacuolation of Helicobacter pylori. J. Microbiol. Biotechnol. 13: 706- 709 
    7. Kim, S. M., K. H. Lee, N. K. Lee, C. J. Kim, C. H. Kim, and H. D. Paik. 2004. Antagonistic activity of polyfermenticin SCD against Helicobacter pylori KCTC 2948. J. Microbiol. Biotechnol. 14: 148-152 
    8. Majumder, R., S. Sengupta, G Khetawat, R. K. Bhadra, S. Roychoudhury, and J. Das. 1996. Physical map of the genome of Vibrio cholerae 569B and localization of genetic markers. J. Bacteriol. 178: 1105 -1112 
    9. Parsonnet, J., S. Hansen, L. Rodriguez, A. B. Gelb, R. A. Warnke, E. Jellum, N. Orentreich, J. H. Vogelman, and G. D. Friedman. 1994. Helicobacter pylori infection and lymphoma. N. Engl. J. Med. 330: 1132-1136 
    10. Shizuya, H., B. W. Birren, U. J. Kim, V. Mancino, T. Slepak, Y. Tachiiri, and Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89: 8794-879 
    11. Van Der Ende, A., Z.-J. Pan, A. Bart, R. W. M. van Der Hulst, M. Feller, S.-D. Xiao, G. N. J. Tytgat, and J. Dankert. 1998. cagA-positive Helicobacter pylori populations in China and The Netherlands are distinct. Infect. Immun. 66: 1822-1826 
    12. Warren, J. R. and B. Marshall. 1983. Unified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1: 1273-1275 
    13. Tomb, J.-F., O. White, A. R. Kerlavage, R. A. Clayton, G G Sutton, R. D. Fleischmann, K. A. Ketchum, H. P. Klenk, S. Gill, B. A. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E. F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H. G Khalak, A. Glodek, E. K. McKenney, L. M. Fitzegerald, N. Lee, M. D. Adams, E. K. Hickey, D. E. Berg, J. D. Gocayne, T. Utterback, I. D. Peterson, J. M. Kelley, M. D. Cotton, J. M. Weidman, C. Fujii, C. Bowman, L. Watthey, E. Wallin, W. S. Hayes, M. Borodovsky, P. D. Karp, H. O. Smith, C. M. Fraser, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539-547 
    14. Aim, R. A., L. S. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397: 176-180 
    15. Aim, R. A. and T. J. Trust. 1999. Analysis of the genetic diversity ofHelicobacter pylori: The tale of two genomes. J. Mol. Med. 77: 834-846 
    16. Baik, S.-C., J.-B. Kim, M.-1. Cho, Y.-c. Kim, C.-G. Park, H.-H. Rhou, H.-J. Choi, and K.-H. Rhee. 1990. Prevalence of Helicobacter pylori infection among normal Korean adults. J. Korean Soc. Microbiol. 25: 455-462 
    17. Ito, Y., T. Azuma, S. Ito, H. Suto, H. Miyaji, Y. Yamazaki, Y. Kohli, and M. Kuriyama. 1998. Full-length sequence analysis of the vacA gene from cytotoxic and noncytotoxic Helicobacter pylori. J. Infect. Dis. 178: 1391-1398 
    18. Kersulyte, D., H. Chalkauskas, and D. E. Berg. 1999. Emergence of recombinant strains of Helicobacter pylori during human infection. Mol. Microbiol. 31: 31-43 
    19. Nedenskov-Sorensen, P., G. Bukholm, and K. Bovre. 1990. Natural competence for genetic transformation in Campylobacter pylori. J. Infect. Dis. 161: 365-366 
    20. Wang, G., M. Z. Humayun, and D. E. Taylor. 1999. Mutation as an origin of genetic variability in Helicobacter pylori. Trends Microbiol. 7: 488-493 
    21. Blaser, M. J. 1998. Helicobacter pylori and gastric diseases. Br. Med. J. 316: 1507-1510 
    22. Marshall, D. G., W. G Dundon, S. M. Beesley, and C. J. Smyth. 1998. Helicobacter pylori - a conundrum of genetic diversity. Microbiology 144: 2925-2939 
    23. Hook-Nikanne, J., D. E. Berg, R. M. Peek Jr., D. Kersulyte, M. K. Tummuru, and M. J. Blaser. 1998. DNA sequence conservation and diversity in transposable element IS605 of Helicobacter pylori. Helicobacter 3: 79-85 
    24. Rhee, K.-H., H.-S. Youn, S.-c. Baik, W.-K. Lee, M.-J. Cho, H.-J. Choi, K.-Y. Maeng, and K.-W. Ko. 1990. Prevalence of Helicobacter pylori infection in Korea. J. Korean Soc. Microbiol. 25: 475-490 
    25. Akopyanz, N., N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg. 1992. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res. 20: 5137-5142 
    26. Rhee, K.-H., M.-J. Cho, J.-B. Kim, S.-K. Choi, C.-K. Park, Y.-C. Kim, J.-H. Choi, and K. J. Choe. 1988. A prospective study on the Campylobacter pylori isolated from patients of gastroduodenal inflammatory conditions. J. Korean Soc. Microbiol. 23: 9-16 
    27. Salama, N., K. Guillemin, T. K. McDaniel, G Sherlock, L. Tompkins, and S. Falkow. 2000. A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains. Proc. Natl. Acad. Sci. USA 97: 14668-14673 
    28. Blaser, M. J. 1990. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J. Infect. Dis. 161: 626-633 
    29. Cave, D. R. 1997. Section I: Epidemiology and transmission of Helicobacter pylori infection - how is Helicobacter pylori transmitted? Gastroenterology 113: S9-14 
    30. Hua, J., H. C. Ng, K. G. Yeoh, and B. Ho. 1999. Predominance of a single strain of Helicobacter pylori in gastric antrum. Helicobacter 4: 28-32 
    31. Shizuya, H., B. W. Birren, U. J. Kim, V. Mancino, T. Slepak, Y. Tachiiri, and Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89: 8794-879 
    32. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, K. Mckenny, G. Sutton, W. Fitzhugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L. Liu, A. Glodek, J. M. Kelly, J. F. Weidman, C. A. Philips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus injluenzae Rd. Science 269: 496-512 
    33. Forman, D., D. G. Newell, F. Fullerton, J. W. Yarnell, A. R. Stacey, N. Wald, and F. Sitas. 1991. Association between infection with Helicobacter pylori and risk of gastric cancer: Evidence from a prospective investigation. Br. Med. J. 302: 1302-1305 
    34. Taylor, D. E., M. Eaton, N. Chang, and S. M. Salama. 1992. Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J. Bacteriol. 174: 6800-6806 
    35. Taylor, N. S., J. G. Fox, N. S. Akopyants, D. E. Berg, N. Thompson, B. Shames, L. Van, E. Fontham, F. Janney, and F. M. Hunter. 1995. Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fngerprinting. J. Clin. Microbiol. 33: 918-923 
    36. Feinberg, A. and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13 
    37. Peterson, W. 1991. Helicobacter pylori and peptic ulcer disease. N. Engl. J. Med. 324: 1043-1048 
    38. Salama, N., K. Guillemin, T. K. McDaniel, G Sherlock, L. Tompkins, and S. Falkow. 2000. A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains. Proc. Natl. Acad. Sci. USA 97: 14668-14673 
    39. Blaser, M. J. 1992. Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102: 720- 727 
    40. Achtman, M., T, Azuma, D. E. Berg, Y. Ito, G. Morelli, Z. J. Pan, S. Suerbaum, S. A. Thompson, A. van der Ende, and L. J. van Doom. 1999. Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol. Microbiol. 32: 459-470 
    41. Chakravarti, D. N., M. J. Fiske, L. D. Fletcher, and R. J. Zagursky. 2000. Application of genomics and proteomics for identification of bacterial gene products as potential vaccine candidates. Vaccine 19: 601-612 
    42. Blaser, M. J. 1987. Gastric Campylobacter-like organisms, gastritis and peptic ulcer disease. Gastroenterology 93: 371-383 
    43. Bukanov, N. O. and D. E. Berg. 1994. Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTCI1638. Mol. Microbiol. 11: 509-523 
    44. Baik, S.-C., H.-S. Youn, M.-H. Chung, W.-K. Lee, M.-1. Cho, G.-H. Ko, C.-G. Park, H. Kasai, and K.-H. Rhee. 1996. Increased oxidative DNA damage in Helicobacter pylori-infected human gastric mucosa. Cancer Res. 56: 1279-1282 
    45. Taylor, D. N. and M. J. Blaser. 1991. The epidemiology of Helicobacter pylori infection. Epidemiol. Rev. 13: 42-59 
    46. Woo, J. S., B. H. Ha, T. G. Kim, Y. Lim, and K. H. Kim. 2003. Inhibition of Helicobacter pylori adhesion by acidic polysaccharide isolated from Artemisia capillaries. J. Microbiol. Biotechnol. 13: 853-858 
    47. Watanabe, T., M. Tada, H. Nagai, S. Sasaki, and M. Nakao. 1998. Helicobacter pylori infection induces gastric cancer in Mongolian gerbils. Gastroenterology 115: 642-648 

 저자의 다른 논문

  • Kang, Hyung-Lyun (14)

    1. 2002 "Analysis of cag Pathogenicity Island of Helicobacter pylori Korean Isolate" Journal of bacteriology and virology : JBV 32 (4): 315~330    
    2. 2003 "A Small Cryptic Plasmid pZMO1 of Zymomonas mobilis ATCC10988" Genomics & informatics 1 (1): 55~60    
    3. 2004 "Enrichment of Low Abundance Proteins of Helicobacter pylori Strain 26695 by the Heparin Chromatography" Journal of bacteriology and virology : JBV 34 (4): 261~272    
    4. 2004 "RFLP Analysis of cag7 Gene of Helicobacter pylori" Journal of bacteriology and virology : JBV 34 (3): 171~180    
    5. 2005 "Application of Hemin-Agarose Affinity Chromatography to Enrich Proteome Components of Helicobacter pylori Strain 26695" Journal of bacteriology and virology : JBV 35 (2): 77~85    
    6. 2005 "Helicobacter pylori Urease Induces Mouse Death" Journal of bacteriology and virology : JBV 35 (3): 175~181    
    7. 2007 "Proteomic Analysis of Helicobacter pylori Whole Cell Proteins using the Narrow Range IPG Strips" Journal of bacteriology and virology : JBV 37 (4): 203~212    
    8. 2011 "Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase" Journal of bacteriology and virology : JBV 41 (4): 255~265    
    9. 2013 "Comparison of Gene Expression Patterns between Helicobacter pylor 26695 and its Superoxide Dismutase Isogenic Mutant" Journal of bacteriology and virology : JBV 43 (4): 279~289    
    10. 2018 "Distinct Genetic Variation of Helicobacter pylori cagA, vacA, oipA, and sabA Genes in Thai and Korean Dyspeptic Patients" Microbiology and biotechnology letters = 한국미생물·생명공학회지 46 (3): 261~268    
  • 이우곤 (34)

  • Park, Seong-Gyu (4)

  • Baik, Seung-Chul (34)

  • 윤희상 (69)

  • Ko, Gyung Hyuck (23)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기