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Journal of microbiology and biotechnology v.15 no.4, 2005년, pp.899 - 902   피인용횟수: 1

Characteristics of Peptide Assimilation by Helicobacter pylori: Evidence for Involvement of Cell Surface Peptidase

YUN SOON-KYU    (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University   ); CHOI KYUNG-MIN    (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University   ); UHM CHANG-SUB    (Department of Anatomy, College of Medicine, Korea University   ); PARK JEONG-KYU    (Department of Microbiology, College of Medicine, Chungnam National University   ); HWANG SE-YOUNG    (Graduate School of Biotechnology & Department of Bioinformatics and Biotechnology, Korea University  );
  • 초록

    Peptide assimilation by Helicobacter pylori was investigated using L-phenylalanyl-3-thia-phenylalanine (PSP) as a detector peptide; the release of thiophenol upon enzymatic hydrolysis of PSP was spectrophotometrically detected with the aid of 5,5'-dithiobis[2-nitrobenzoic acid] (DTNB). By adding PSP to whole-cell suspension, thiophenol was produced progressively, resembling that found in Esherichia coli or Staphylococcus aureus. Interestingly, the rate of thiophenol production by H pylori in particular was markedly reduced when cells were pretreated with trypsin, indicating surface exhibition of peptidase. According to the competitive spectrophotometry using alanyl-peptides, H pylori did not appear to assimilate PSP through the peptide transport system. No discernible PSP assimilation could be ascertained in H pylori cells, unless provided with some additives necessary for peptidase activity, such as $Ni^{2+}\;or\;Mg^{2+}$ and an appropriate concentration of potassium or ammonium salts. These observations strongly suggest that, regardless of a presumptive peptide transport system, peptide assimilation of H. plori appears to be highly dependent upon milieu conditions, due to unique peptidase exhibition on the cell surface.


  • 주제어

    Helicobacter pylori .   peptide transport .   detector peptide .   peptidase.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2006. "" Journal of microbiology and biotechnology, 16(7): 1078~1083     

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