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Responsiveness to Lipopolysaccharide Changes According to the Aging of Periodontal Ligament Fibroblasts

Jun, Ji-Hae    (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Dental Research Institute, Seoul National University   ); Kim, Gwan-Shik    (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Dental Research Institute, Seoul National University   ); Woo, Kyung-Mi    (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Dental Research Institute, Seoul National University   ); Min, Byung-Moo    (Department of Oral Biochemistry, College of Dentistry, Dental Research Institute, Seoul National University   ); Baek, Jeong-Hwa    (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Dental Research Institute, Seoul National University  );
  • 초록

    The elderly suffer from an impaired immune function being obvious in a higher susceptibility to infections. Although the inflammatory cells are the major immunomodulatory cells, fibroblasts also secrete a variety of inflammatory cytokines and chemokines. Therefore periodontal tissue aging might playa role in development and progress of periodontitis. In this study, we investigated the effect of in vitro periodontal ligament cellular aging on the inflammatory cytokines, chemokines, and matrix metalloprotease(MMP)-2 expression induced by lipopolysaccharide(LPS) treatment. Three different cell populations were used; passages 4-5, 14-15, and 24-25 (at passage 27, more than 90% cells were replicative senescent). LPS increased the expression of interleukin(IL)-1 ${\beta}$ , IL-6, and tumor necrosis factor- ${\alpha}$ , IL-8, RANTES, and MMP-2. However, the order of induction folds were passages 14-15 > 4-5 > 24-25. While the expression level of Toll-like receptor(TLR) 4 decreased according to the increase in passage number, the level of TLR2 was highest at passages 14-15 and then decreased at passages 24-25. While the spontaneous expression of IL-8 decreased according to the increase in passage number, that of RANTES and proMMP-2 increased according to the increase in passage number. These results suggest that the aging of periodontal ligament fibroblasts differentially affect the role as immunomodulatory cells in response to periodontopathic bacteria and therefore might be another risk factor of periodontitis progression.


  • 주제어

    In vitro aging .   Periodontal ligament fibroblasts .   Lipopolysaccharide .   Inflammatory cytokine .   Chemokine .   Matrix metalloprotease-2 .   Toll-like receptor.  

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    1. 2007. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 32(1): 35~43     

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