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Effects of Achyranthes Radix Extracts on Osteoblasts and Osteocalsts

Kim, Kang-Yong    (Dept. of Oral Biochemistry, Dankook University   ); Kim, Se-Won    (Dept. of Dental Pharmacology, Dankook University   ); Kim, Jung-Keun    (Dept. of Oral Biochemistry, Dankook University   ); Ko, Seon-Yle    (Dept. of Oral Biochemistry, Dankook University  );
  • 초록

    The present study was performed to investigate whether Achyranthes Radix extracts play roles in the bone metabolism. Three kinds of Achyranthes Radix extracts (methylene chloride (MC), ethylacetate (Ea), and water (W)) were used for bioassay. We examined cellular activities of osteoblasts by measurement of cell proliferation rate, alkaline phosphatase (ALP) activity, and calcified nodule formation. Osteoclast generation was assayed by measuring the number of tartrate-resistant acid phosphatase (TRAP) (+) multinucleated cells after culture of osteoclast precursor cells. There was a maximum 20% increase in proliferation rate of osteoblastic cells after treatment with MC. First and second subfraction of MC layer increased proliferation of osteoblast. Ea layer and second subfraction of MC layer increased ALP activity. Also MC layer and second subfraction of MC layer from Achyranthes Radix extracts increased the calcified nodule. MC layer and second subfraction of MC layer from Achyranthes Radix extracts significantly decreased in the number of TRAP (+) multinucleated cells. Taken together, Achyranthes Radix stimulates the proliferation and bioactivities of bone-forming osteoblasts, and inhibits activities of bone-resorbing osteoclasts.


  • 주제어

    Achyranthes Radix .   Osteoblast .   Osteoclast.  

  • 참고문헌 (42)

    1. Akhtar, M.S. and Iqbal, J.: Evaluation of the hypoglycemic effect of Achyranthes aspera in normal and alloxan-diabetic rabbits. J. Ethnopharmacology 31:49-57, 1991 
    2. Cryer, R. and Bauer, D.C.: Oral bisphosphonates and upper gastrointestinal tract problem: what is the evidence? Mayo Clin. Proc. 77: 1031-1043, 2002 
    3. Farley, J.R. and Baylink, D.: Skeletal alkaline phosphatase activity as a bone formation index in vitro. Metabolism 35:563-571, 1986 
    4. Genant, H.K., Baylink, D.J. and Gallagher, J.C.: Estrogens in the prevention of osteoporosis in postmenopausal women. Am. J. Obest. Gynecol. 161:1842-1846, 1989 
    5. John, D.: One hundred useful raw drugs of the Kani tribes of Trivandrum, Forest division, Kerala State, India. Int. J. Crude Drug Res. 22: 17-39, 1984 
    6. Khurana, S.M.P. and Bhagva, K.S.: Effect of plant extracts on the activity ofthree papaya viruses. J. Gen. Appl. Microbiol. 16:225-230, 1970 
    7. Kirtikar, K.R, and Basu, B.D.: Indian medicinal plants, L.M. p 2066, Allahabad, 1935 
    8. Lu, T., Mao, C., Zhang, L. and Xu, W.: The research on analgestic and anti-inflammatory action of different processed products of Achyranthes bidentata. Zhong Yao Cai. 20:507-509, 1997 
    9. Ratra, P.S.: Alkaloids in two species of Achyranthes at different stages of their growth. Trends Sci. Life 4:81-85, 1979 
    10. Reginster, J.Y.: Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin. Horm. Res. 43:83-88, 1995 
    11. Rodan, G.A.: Emerging therapies in osteoporosis. Ann. Rep. Med. Chem. 29:275-285, 1994 
    12. Vetrichelvan, T. and Jegadeesan, M.: Effect of alchol extract of Achyranthes aspera Linn. on acute and subacute inflammation. Phytotherapy Res. 17:77-79, 2003 
    13. Satyavati, G.V, Raina, M.K. and Sharma, M.: Medicinal Plants of India, vol 1. Indian Council of Medical Research, New Delhi, pp 10-14, 1976 
    14. Gao, X.Y., Wang, D.W. and Li, F.M.: Determination of ecdysterone in Achyranthes bidentata B1. and its activity promoting proliferation of osteoblast-like cells. Yao Xue Xue Bao. 35:868-870, 2000 
    15. Recker, R.P.: Current therapy for osteoporosis. J. Clin. Endocrinol. Metab. 76:14-16, 1993 
    16. Reddy, M.B., Reddy, K.R. and Reddy, M.N.: A survey of plant crude drugs of Anantapur District, Andhrapradesh, India, Int. J Crude Drug Res. 27: 145-155, 1989 
    17. Seshatri, V, Barta, A.K. and Rangaswami, S.: Structure of two new saponins from A chyranthes aspera. Indian J. Chem. Ser. 20:773-775, 1981 
    18. Nijweide, P.J., Burger, E.H. and Feyen, J.H.M.: Cells of bone: Proliferation, differentiation, and hormonal regulation. Physiol. Rev. 66:855-886, 1986 
    19. Ojha, D. and Singh, G.C.: Achyranthes aspera in the treatment of lepromatous leprosy. Leprosy Rev. 39:23, 1968 
    20. Kapoor, V.K. and Singh, H.: Isolation of betaine from Achyranthes aspera. Indian J. Chem. 4:461, 1966 
    21. Nelson, H.D, Humphrey, L.L., Nygren, P., Teutsch, S.M., and Allan, J.D.: Postmenopausal hormone replacement therapy: scientific review. JAMA 288:872-881, 2002 
    22. Mundy, G.R. and Roodman, G.D.: Osteoclast ontogeny and function, In Bone and Mineral Research, vol. 5, edited by Peck, W.A., pp 209-279, Elsevier, Amsterdam, 1987 
    23. Takahashi, N., Yamana, H., Yoshiki, S., Roodman, G.D., Mundy, G.R., Jones, S.J., Boyde, A. and Suda, T.: Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology 122:1373-1382, 1988 
    24. Gessler, M.C., Nkunya, M.H., Mwasumbi, L.B., Heinrich, M. and Tanner, M.: Screening Tanzanian medicinal plants for antimalarial activity. Acta Trop. 56:65-77, 1994 
    25. Jain, S.P. and Puri, H.S.: Ethnomedical plants of Jaunsar-Bawar hills Uttar Pradesh, India. J. Ehnopharmacol. 12:213- 222, 1984 
    26. Nadkami, K.M. and Nadkami, A.K.: Indian materia Medica, vol 1., Popular Prakashan, Bombay, p21, 1976 
    27. Bhattari, M.K.: Medical ethnobotany in the Rapti zone. Nepal. Fitoterapia 64:483-489, 1993 
    28. Weinreb, M., Rodan, G.A. and Thompson, D.D.: Osteopenia in the immobilized rat hind limb is associated with increased bone resorption and decreased bone formation. Bone 10:187-194, 1989 
    29. Desta, B.:Ethiopian traditional herbal drugs. Part II: Antimicrobial activity of 63 medicinal plants. J. Ethnopharmacol. 39:129-39, 1993 
    30. Tian, G.Y, Li, S.T., Song, M.L., Zheng, M.S. and Li, W.: Synthesis of Achyranthes bidentata polysaccharide sulfate and its antivirus activity. Yao Xue Xue Bao. 30:107-111, 1995 
    31. Lemay, A.: The relevance of the women's health initiative results on combined hormone replacement therapy in clinical practice. J. Obstet. Bynaecol. Can. 24:711-715, 2002 
    32. Kong, Y.C., Hu, S.C., and Lau, F.K.: Potential antifertility plants from Chinese medicine. Am. J. Chin. Med. 4: 105-128, 1976 
    33. Wadhwa, V., Singh, M.M., Gupta, D.N., Singh, C. and Kamboj, V,P.: Contraceptive and hormonal properties of Achyranthes aspera in rats and hamsters. Planta Med. 3:231-233, 1986 
    34. Heaney, R.P.: Calcium, In Principles of Bone biology, edited by Bilezikian, J.P., Raisz, L.G. and Rodan, G.A., pp 1007-1017, Academic Press, USA, 1996 
    35. Singh, Y.N.: Traditional medicine in Fiji, some herbal folk cures used by Fiji indians. J. Ethnopharmacol. 15: 57-88, 1986 
    36. Frost, H.M.: Dynamics of bone remodeling, In Bone Biodynamics, pp 315, Little and Brown, Boston, MA, USA, 1964 
    37. Minkin, C.: Bone acid phosphatase: Tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif. Tissue Int. 34:285-290, 1982 
    38. Raman, M.H. and Faroque, A.S.M.: Studies on the antibacterial properties of Achyranthes aspera stem. Fitoterapia LXII: 92-93, 1996 
    39. Xiang, D.B. and Li, X.Y.: Effects of Achyranthes bidentata polysaccharides on interleukin-l and tumor necrosis factoralpha production from mouse peritoneal macrophages. Zhongguo Yao Li Xue Bao. 14:332-336, 1993 
    40. Khanna, A.K., Chandra, A., Singh, C., Srivastava, A.K. and Kapoor, N.: Hypolipidamic activity of Achyranthes aspera Linn. in normal and triton induced hyperlipidamic rats. J. Exp. Biol. 30: 128-130, 1992 
    41. Stein, G.S., Lian, J.B., Stein, J.L., van Wijnen, A.J. and Montecino, M.: Transcriptional control of osteoblast growth and differentiation. Physiol. Rev. 76:593-625, 1996 
    42. Fleisch, H.: Bisphosphonates : Mechanism of action and clinical use, In Principles of Bone biology, edited by Bilezikian, J.P., Raisz, L.G. and Rodan, G.A., pp 1007-1017, Academic Press, USA, 1996 

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