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GABAergic Synaptic Input to Mesencephalic Trigeminal Neurons in Rat

Ryu, Hyo-Chel    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Piao, Zheng Gen    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Choi, Se-Young    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Lee, Sung-Joong    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Park, Kyung-Pyo    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Kim, Joong-Soo    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University   ); Oh, Seog-Bae    (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University  );
  • 초록

    The mesencephalic trigeminal nucleus (Mes V) contains cell bodies of primary afferent sensory neurons that relay proprioceptive information from the periodontium and masticatory muscles and function as typical sensory neurons or potentially as integrative interneurons. In the present study, we studied these two potential functions using combined experimental approaches of retrograde labeling and whole cell patch clamp recording. Mes V neurons that presumably originate from periodontal nerve fibers in subsets of Mes V nucleus were identified by retrograde labeling with a fluorescent dye, DiI, which was applied onto inferior alveolar nerve. These cells were elliptical perikarya shaped cells about $40{\mu}m$ in diameter. In these neurons, we measured high voltage-activated calcium channel (HVACC) currents. $GABA_B$ agonist, baclofen, inhibited calcium currents, and the HVACC currents inhibition by baclofen was voltage-dependent, exhibited prepulse facilitation, indicating that it was mediated by $G_i/_G_o$ protein. Taken together, our results demonstrate that Mes V neurons not only have cell bodies originating from periodontium, but also receive synaptic inputs including GABAergic neurons suggesting that Mes V neurons function as both primary sensory neurons and integrative interneurons.


  • 주제어

    mesencephalic trigeminal nucleus .   retrograde labeling .   DiI .   whole-cell patch clamp recording .   calcium currents.  

  • 참고문헌 (28)

    1. Bae, Y. C., Park, K. P., Yoshida, A., Nakagawa, S., Kurata, S., Chen, K., Takemura, M. and Shigenaga, Y. Identifi-cation of gamma-aminobutyric acid-immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus. J Comp Neurol 389:127-138, 1997 
    2. Hinrichsen, C. F. & Larramendi, L. M. Synapses and cluster formation of the mouse mesencephalic fifth nucleus. Brain Res 7:296-299, 1968 
    3. Kayahara, T., Sakashita, S. & Takimoto, T. Evidence for spinal origin of neurons synapsing with dorsal root ganglion cells of the cat. Brain Res 293:225-230, 1984 
    4. Kolta, A., Lund, J. P. & Rossignol, S. Modulation of activity of spindle afferents recorded in trigeminal mesen-cephalic nucleus of rabbit during fictive mastication. J Neurophysiol 64:1067-1076, 1990 
    5. Lazarov, N. E. Comparative analysis of the chemical neuroanatomy of the mammalian trigeminal ganglion and mesencephalic trigeminal nucleus. Prog Neurobiol 66:19- 59, 2002 
    6. Liem, R. S., Copray, J. C. and Van Willigen, J. D. Distribution of synaptic boutons in the mesencephalic trigeminal nucleus of the rat--a quantitative electron-microscopical study. Acta Anat (Basel) 143:74-78, 1992 
    7. Witkovsky, P. and Roberts, B. L. Electron microscopic observations of the mesencephalic nucleus of the fifth nerve in the Selachian brain. J Neurocytol 5:643-660, 1976 
    8. Hinrichsen, C. F. and LARRAMENDI, L. M. The trigeminal mesencephalic nucleus. II. Electron microscopy. Am J Anat 127:303-319, 1970 
    9. Liem, R. S., Copray, J. C. and Van Willigen, J. D. Ultra-structure of the rat mesencephalic trigeminal nucleus. Acta Anat (Basel) 140:112-119, 1991 
    10. Dessem, D., Donga, R. and Luo, P. Primary- and secondary-like jaw-muscle spindle afferents have characteristic topographic distributions. J Neurophysiol 77:2925-2944, 1997 
    11. Yang, B. H., Piao, Z. G, Kim, Y. B., Lee, C. H., Lee, J. K., Park, K., Kim, J. S. and Oh, S. B. Activation of vanilloid receptor 1 (VR1) by eugenol. J Dent Res 82:781-785, 2003 
    12. Linden, R. W., Millar, B. J. and Halata, Z. A comparative physiological and morphological study of periodontal ligament mechanoreceptors represented in the trigeminal ganglion and the mesencephalic nucleus of the cat. Anat Embryol (Berl) 190:127-135, 1994 
    13. Lazarov, N. E. and Chouchkov, C. N. Immunocyto-chemical localization of tyrosine hydroxylase and gamma-aminobutyric acid in the mesencephalic trigeminal nucleus of the cat: a light and electron microscopic study. Anat Rec 242:123-131, 1995 
    14. Oh, S. B., Piao, Z. G, Shin, S. S., Ren, D., Park, K. and Kim, J. S. GABAergic and serotonergic modulation of calcium currents in rat trigeminal motoneurons. Biochem Biophys Res Commun 309:58-65, 2003 
    15. Yamamoto, M. and Kondo, H. Calcitonin gene-related peptide (CGRP)-immunoreactive nerve varicosities in synaptic contact with sensory neurons in the trigeminal ganglion of rats. Neurosci Lett 104:253-257, 1989 
    16. Luo, P. and Dessem, D. Morphological evidence for recurrent jaw-muscle spindle afferent feedback within the mesencephalic trigeminal nucleus. Brain Res 710:260-264, 1996 
    17. Hille, B., Beech, D. J., Bernheim, L., Mathie, A., Shapiro, M. S. and Wollmuth, L. P. Multiple G-protein-coupled pathways inhibit N-type Ca channels of neurons. Life Sci 56:989-992, 1995 
    18. Peng, Y. Y. and Frank, E. Activation of GABAB receptors causes presynaptic inhibition at synapses between muscle spindle afferents and motoneurons in the spinal cord of bullfrogs. J Neurosci 9:1502-1515, 1989 
    19. Gottlieb, S., Taylor, A. & Bosley, M. A .. The distri-bution of afferent neurones in the mesencephalic nucleus of the fifth nerve in the cat. J Comp Neurol 228:273-283, 1984 
    20. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85-100, 1981 
    21. Levy, R. A. The role of GABA in primary afferent depolarization. Prog Neurobiol 9:211-267, 1977 
    22. Hassanali, J. Quantitative and somatotopic mapping of neurones in the trigeminal mesencephalic nucleus and ganglion innervating teeth in monkey and baboon. Arch Oral Biol 42:673-682, 1997 
    23. Zhang, J. D., Yoshida, A. and Shigenaga, Y. Ultra-structural analysis of inputs around the soma of an intra-cellularly labeled masseter muscle spindle afferent in cat mesencephalic trigeminal nucleus. J Hirnforsch 38:495-502, 1997 
    24. Nomura, S. and Mizuno, N. Differential distribution of cell bodies and central axons of mesencephalic trigeminal nucleus neurons supplying the jaw-closing muscles and periodontal tissue: a transganglionic tracer study in the cat. Brain Res 359:311-319, 1985 
    25. Zamponi, G. W. and Snutch, T. P. Modulation of voltage-dependent calcium channels by G proteins. Curr Opin Neurobiol 8:351-356, 1998 
    26. Hayar, A., Poulter, M. O., Pelkey, K., Feltz, P. and Marshall, K. C. (1997). Mesencephalic trigeminal neuron responses to gamma-aminobutyric acid. Brain Res 753:120-127 
    27. Kayahara, T., Takimoto, T. & Sakashita, S. Synaptic junctions in the cat spinal ganglion. Brain Res 216:277-290, 1981 
    28. Ikeda, S. R. Double-pulse calcium channel current facilitation in adult rat sympathetic neurones. J Physiol (Lond) 439: 181-214, 1991 

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