본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Effect of TGF-${\beta}1$ on Osteoclast Differentiation

Park, Su-Jin   (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National UniversityUU0000691  ); Ko, Jea-Seung   (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National UniversityUU0000691  ); Kim, Hyun-Man   (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National UniversityUU0000691  );
  • 초록

    Although it has been known that TGF- ${\beta}1$ acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF- ${\beta}1$ on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF- ${\kappa}B$ ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF- ${\beta}1$ stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF- ${\beta}1$ in the early stage than the later differentiation. TGF- ${\beta}1$ increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF- ${\kappa}B$ MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF- ${\beta}1$ may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation.


  • 주제어

    osteoclast .   TGF- ${\beta}$ .   differentiation .   bone.  

  • 참고문헌 (34)

    1. Hughes, D. E., Dai, A., Tiffee, J. C., Li, H. H., Mundy, G. R., and Boyce, B. E: Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-$\beta$. Nat. Med. 2:1132-1136, 1996 
    2. Karsdal, M. A., Fjording, M. S., Foged, N. T., Delaisse, J. M., and Lochter, A.: Transforming growth factor-beta-induced osteoblast elongation regulates osteoclastic bone resorption through a p38 mitogen-activated protein kinase- and matrix metalloproteinase-dependent pathway. J. Biol. Chem. 276:39350-39358, 2001 
    3. Matsumoto, M., Sudo, T., Saito, T., Osada, H and Tsujimoto, M.: Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J Biol Chem. 275:31155-31161, 2000 
    4. Pelton, R. W., Saxena, B., Jones, M., Moses, H. L., and Gold, L. I.: Immunohistochemical localization of TGF-$\beta$1, TGF-$\beta$2, and TGF-$\beta$3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development. J. Cell Biol. 115:1091-1105, 1991 
    5. Quinn, J. M., Itoh, K., Udagawa, N., Hausler, K., Yasuda, H., Shima, N., Mizuno, A., Higashio, K., Takahashi, N., Suda, T., Martin, T. J., and Gillespie, M. T.: Transforming growth factor beta affects osteoclast differentiation via direct and indirect actions. J. Bone Miner. Res. 16:1787-1794, 2001 
    6. Karsdal, M. A., Larsen, L., Engsig, M. T., Lou, H., Ferreras, M., Lochter, A., Delaisse, J. M., and Foged, N. T.: Matrix metalloproteinase-dependent activation oflatent transforming growth factor-beta controls the conversion of osteoblasts into osteocytes by blocking osteoblast apoptosis. J. Biol. Chem. 277:49343-49349, 2001 
    7. Matsumoto, M., Sudo, T., Maruyama, M., Osada, H and Tsujimoto, M.: Activation of p38 mitogen-activated protein kinase is crucial in osteoclastogenesis induced by tumor necrosis factor. FEBS Lett. 486:23-28, 2000 
    8. Yan, T., Riggs, B. L., Boyle, W. J. and Khosla, S.: Regulation of osteoclastogenesis and RANK expression by TGF-beta1. J Cell Biochem. 83:320-325, 2001 
    9. Marcelli, C., Yates, A. J., and Mundy, G. R.: In vivo effects of human recombinant transforming growth factor beta on bone turnover in normal mice. J. Bone Miner. Res. 5:1087-1096, 1990 
    10. Takai, H., Kanematsu, M., Yano, K., Tsuda, E., Higashio, K., Ikeda, K., Watanabe, K., and Yamada, Y.: Transforming growth factor $\beta$ stimulates the production of osteoprotegerin/osteoclastogenesis inhibitory factor by bone marrow stroma cells. J. Biol. Chem. 273:27091-27096, 1998 
    11. Murakami, T., Yamamoto, M., Ono, K., Nishikawa, M., Nagata, N., Mytoyoshi, K., and Akatsu, T.: Transforming growth factor-zil increases mRNA levels of osteoclasto-genesis inhibitory factor in osteoblastic/stroma cells and inhibits the survival of murine osteoclast-like cells. Biochem. Biophys. Res. Commun. 252:747-752, 1998 
    12. Kim, H. H., Chung, W. J., Lee, S. W., Chung, P. J., You, J. W., Kwon, H. J., Tanaka, S., and Lee, Z. H.: Association of sustained ERK activity with integrin beta3 induction during receptor activator of nuclear factor kappaB ligand (RANKL)-directed osteoclast differentiation. Exp Cell Res. 289:368-77, 2003 
    13. Kato, M.: Transcriptional regulation by the transforming growth factor-beta signaling. Tanpakushitsu Kakusan Koso. 48:2247-2253, 2003 
    14. Tanaka, S., Nakamura, I., Inoue, J., Oda, H., and Nakamura, K.: Signal transduction pathways regulating osteoclast differentiation and function. J Bone Miner Metab. 21:123-33, 2003 
    15. Fuller, K. Lean, J. M., Bayley, K. E., Wani, M. R., and Chambers, T. J.: A role for TGF-$\beta$1 in osteoclast differentiation and survival. J. Cell Sci. 113:245-253, 2000 
    16. Sandberg, M., Vuorio, T., Hirvonen, H., Alitalo, K., and Vuorio, E. Enhanced expression of TGF-$\beta$ and c-fos mRNA in the growth plates of developing human long bones. Development. 102:1461-1470, 1988 
    17. Kim, H. H., Lee, D.E., Shin, J. N., Lee, Y. S., Jeon, Y. M., Chung, C. H., Ni, J., Kwon, B. S., and Lee, Z. H.: Receptor activator of NF-kappaB recruits multiple TRAF family adaptors and activates c-Jun N-terminal kinase. FEBS Lett. 443:297-302, 1999 
    18. Mizukami, J., Takaesu, G., Akatsuka, H., Sakurai, H., Ninomiya-Tsuji, J., Matsumoto, K., Sakurai, N.: Receptor activator of NF-kappa B ligand (RANKL) activates TAK1 mitogen-activated protein kinase kinase kinase through a signaling complex containing RANK, TAB2, and TRAF6. Mol Cell Biol. 22:992-1000, 2002 
    19. Seyedin, S. M., Thompson, A. Y., Bentz, H., Rosen, D. M., Mcpherson, J. M., Conti, A., Siegel, N. R., Galluppi, G. F., and Piez, K. A.: Cartilage-inducing factor-A : apparent identity to transforming growth factor-$\beta$, J. Biol. Chem. 261:5693-5695, 1986 
    20. Zhang, L., Duan, C. J., Binkley, C., Li, G., Uhler, M. D., Logsdon, C. D., and Simeone, D. M.: A transforming growth factor beta-induced Smad3/Smad4 complex directly activates protein kinase A. Mol Cell Biol. 24:2169-2180, 2004 
    21. Li, X., Udagawa, N., Itoh, K., Suda, K., Murase, Y., Nishihara, T., Suda, T and Takahashi, N.: p38 MAPK-mediated signals are required for inducing osteoclast differentiation but not for osteoclast function. Endocrinology. 143:3105-3113, 2002 
    22. Toshio, K., Takaki, N., Mari, N., Aichi, O., Hirnori. K., Takuya, S., Hiroshi, M., Masayoshi, K., and Yoshiyuki, H.: Endogenous production of TGF-$\beta$ is essential for osteoclastogenesis induced by a combination of Receptor Activator of NF-$\kappa$B Ligand and Macrophage-Colony stimulating Factor. J. Immunol. 165:4254--4263, 2000 
    23. Matsumoto, M., Sudo, T., Saito, T., Osada, H., and Tsujimoto, M.: Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J Biol Chem. 275:31155-61, 2000 
    24. Hauschka, P. V., Mavrakos, A. E., Iafrati, M. D., Doleman, S. E., and Klagsbrun, M.: Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose. J. Biol. Chem. 261:12665-12674, 1986 
    25. Lee, S. W., Han, S. I., Kim, H. H., and Lee, Z. H.: TAK1-dependent activation of AP-l and c-Jun N-terminal kinase by receptor activator of NF-kappaB. J Biochem Mol Biol. 35:371-376, 2002 
    26. Erlebacher, A. and Derynck, R.: Increased expresiion of TGF-$\beta$2 in osteobalsts results in an osteoporosis-like phenotypes. J. Cell Biol. 132:195-210, 1996 
    27. Bonewald, L. F. and Mundy, G. R.: Role of transforming growth factor-beta in bone remodeling. Clin. Orthop. 250:261-276, 1990 
    28. Filvaroff, E. H., Ebner, R., and Derynck, R.: Inhibition of myogenic differentiation in myoblasts expressing a truncated type II II TGF-$\beta$ receptor. Development. 120:1085-1095, 1994 
    29. Robey, P. G., Young, M. F., Flanders, K. C., Roche, N. S., Kondaiah, P., Reddi, A., Termine, J. D., Sporn, M. B., and Roberts, A. N.: Osteoblasts synthesize and respond to transforming growth factor-type $\beta$(TGF-$\beta$) in vitro. J. Cell Biol. 105:457-463, 1987 
    30. Dallas, S. L., Rosser, J. L., Mundy, G. R., and Bonewald, L. F.: Proteolysis of latent transforming growth factor-beta (TGF-$\beta$)-binding protein-l by osteoclasts. A cellular mechanism for release of TGF-beta from bone matrix. Biol. Chem. 277:21352-21360, 2002 
    31. Javelaud, D. and Mauviel, A.: Transforming growth factorbetas: smad signaling and roles in physiopathology. Pathol Biol (Paris). 52:50-54, 2004 
    32. Massaque, J.: The transforming growth factor-beta family. Ann. Rev. Cell. Biol. 6:597-609, 1990 
    33. Fox, S. W., Fuller, K., Bayley, K.E., Lean, J. K., and Chambers, T. J.: TGF-beta 1 and IFN-gamma direct macrophage activation by TNF-alpha to osteoclastic or cytocidal phenotype. J. Immunol. 165:4957-4963, 2000 
    34. Karsdal, M. A., Hjorth, P., Henriksen, K., Kirkegaard, T., Nielsen, K. L., Lou, H., Delaisse, J. M and Foged, N. T.: Transforming growth factor-$\beta$ controls human osteoclastogenesis through the p38 MAPK and regulation of RANK expression. J Biol Chem. 27:44975-44987, 2003 

 저자의 다른 논문

  • 고재승 (16)

    1. 1980 "테트라싸이클린의 경구투여가 백서의 치주염에 미치는 영향에 관한 조직학적 연구" 대한치주과학회지 = The journal of Korean academy of periodontology 10 (1): 41.2~41.2    
    2. 1980 "신선 상악골 및 대퇴골 이식에 관한 연구" 大韓齒科醫師協會誌 = The journal of the Korean dental association 18 (2): 109~114    
    3. 1990 "상아질 지각과민증 치료후 임상 및 주사전자현미경적 관찰" 대한치주과학회지 = The journal of Korean academy of periodontology 20 (2): 163~163    
    4. 1991 "LOADED IMPLANT와 UNLOADED IMPLANT의 조직학적 비교 연구" 대한치과보철학회지 = The journal of Korean academy of prosthodontics 29 (2): 1~16    
    5. 1993 "실험가토의 악관절원판 변위시 후방부착조직의 변화" 대한치과교정학회지 = The Korean journal of orthodontics 23 (4): 503~527    
    6. 1993 "테트라싸이클린이 치주결체조직의 재부착에 미치는 효과" 대한치주과학회지 = The journal of Korean academy of periodontology 23 (2): 203~218    
    7. 1993 "구연산, 테트라싸이클린, 섬유소 전색제로 처리된 상아질면에 대한 결체조직의 조기부착" 대한치주과학회지 = The journal of Korean academy of periodontology 23 (2): 283~299    
    8. 1996 "생체요업재료와 차폐막의 복합사용후 골연하 결손부의 재생효과" 대한치주과학회지 = The journal of Korean academy of periodontology 26 (1): 47~67    
    9. 1996 "발치와의 육아조직 이식이 치근이개 결손부의 재생에 미치는 영향" 대한치주과학회지 = The journal of Korean academy of periodontology 26 (3): 735~751    
    10. 1997 "($CO_2$)레이저 치료에 의한 치근표면의 구조적 변화" 대한치주과학회지 = The journal of Korean academy of periodontology 27 (3): 469~478    
  • Kim, Hyun-Man (12)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기