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Food science and biotechnology v.14 no.1 = no.61, 2005년, pp.171 - 174   피인용횟수: 5

Analysis of Flavonoids in Concentrated Pomegranate Extracts by HPLC with Diode Array Detection

Lee, Jeong-Hwan   (Institute of Food and Culture Research, Pulmuone Co., Ltd.  ); Kim, Seung-Deok   (Institute of Food and Culture Research, Pulmuone Co., Ltd.  ); Lee, Ja-Young   (Institute of Food and Culture Research, Pulmuone Co., Ltd.  ); Kim, Kyung-Nam   (Institute of Food and Culture Research, Pulmuone Co., Ltd.  ); Kim, Hyun-Su   (Institute of Food and Culture Research, Pulmuone Co., Ltd.  );
  • 초록

    Three flavonoid compounds - quercetin, luteolin, and kaempferol - were analyzed from two commercially available concentrated pomegranate extracts produced in Turkey and Italy, respectively. The samples were freeze-dried and hydrolyzed by 0.4 M hydrochloric acid in 50% ethanol at $80^{\circ}C$ . HPLC (high-performance liquid chromatography) with DAD (diode array detection) at a wavelength of 260 nm was used for the detection of the three flavonoids. The detection limits of the three compounds were in hundreds of picograms and the signal-to-noise ratio ranged from 4 to 5: quercetin: >308 pg, s/n=4.0; luteolin: >262 pg, s/n=4.5; kaempferol: >688 pg, s/n=5.0. Quercetin, but not luteolin and kaempferol, was detected in the both pomegranate extracts. The concentrations of quercetin were $49.7\;{\mu}g/g$ and $27.7\;{\mu}g/g$ in the two pomegranate extracts made in Turkey and Italy, respectively.

  • 주제어

    pomegranate .   quercetin .   flavonoid .   HPLC.  

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  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" Food science and biotechnology, 15(5): 735~738     
    2. 2006. "" Food science and biotechnology, 15(6): 959~966     
    3. 2007. "" Food science and biotechnology, 16(5): 740~746     
    4. 2007. "" Food science and biotechnology, 16(5): 828~831     
    5. 2010. "" Food science and biotechnology, 19(3): 809~813     

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