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Food science and biotechnology v.14 no.2 = no.62, 2005년, pp.263 - 267   피인용횟수: 3

Isolation of Hyaluronidase Inhibitory Component from the Roots of Astraglus membranaceus Bunge (Astragali Radix)

Lee, Yun-Mi    (Department of Biological Engineering, Inha University   ); Choi, Soo-Im    (Department of Biological Engineering, Inha University   ); Lee, Jae-Won    (Department of Biological Engineering, Inha University   ); Jung, Sun-Mi    (Department of Biological Engineering, Inha University   ); Park, Sang-Min    (Department of Biological Engineering, Inha University   ); Heo, Tae-Ryeon    (Department of Biological Engineering, Inha University  );
  • 초록

    In order to isolate hyaluronidase (HAase) inhibitor from Astragali radix (AR), dried roots were extracted with ethanol, prior to sequential fractionations with n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. The n-butanol soluble fraction was found to exhibit the most pronounced inhibitory effect (68%) on HAase, and the active components were separated using various chromatographic methods, including column chromatography and preparative HPLC. The active component was isolated from the n-butanol soluble fraction of AR and was structurally identified as calycosin-7-O- ${\beta}$ -D-glucopyranoside by LC-MS, IR, $^1H$ NMR, and $^{13}C$ NMR analysis. The $IC_{50}$ of calycosin-7-O- ${\beta}$ -D-glucopyranoside's HAase activity was found to be 3.7 mg/mL.


  • 주제어

    Astragalus membranaceus Bunge .   calycosin-7-O- ${\beta}$-D-glucopyronoside .   hyaluronidase inhibitor.  

  • 참고문헌 (19)

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  • 이 논문을 인용한 문헌 (3)

    1. 2007. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 30(10): 1318~1327     
    2. 2008. "" Food science and biotechnology, 17(1): 38~45     
    3. 2008. "" Food science and biotechnology, 17(2): 402~407     

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