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Food science and biotechnology v.14 no.2 = no.62, 2005년, pp.297 - 300   피인용횟수: 1

Mixed-type Inhibition of Human Hepatic Cytochrome P450 1-Catalyzed Ethoxyresorufin O-deethylation by Volatile Allyl Sulfides

Kim, Hyun-Jung    (Korea Food Research Institute   ); Chun, Hyang-Sook    (Korea Food Research Institute  );
  • 초록

    Effects of allyl sulfides on kinetic behavior of cytochrome P450 1 (CYP1)-catalyzed ethoxyresorufin O-deethylase (EROD) activity were studied using microsomes from benzo[a]pyrene-treated human hepatoma cells. Apparent $K_m$ and $V_{max}$ values were calculated as $2.8\;{\mu}M$ and $3.0\;{\mu}mol$ resorufin/min/mg protein based on Lineweaver-Burk plot of microsomal EROD activity, respectively. Diallyl disulfide (DADS) and diallyl trisulfide (DATS) affected $K_m$ and $V_{max}$ values of EROD activity and acted as mixed-type inhibitors for CYP1 isozymes. Apparent Ki values of DADS and DATS were calculated as 1.07 and 0.88 mM, respectively, by re-plotting slopes of Lineweaver-Burk plot and inhibitor concentrations.


  • 주제어

    allyl sulfides .   benzo[a]pyrene .   cytochrome P450 1 .   human hepatoma cell .   mixed-type inhibition.  

  • 참고문헌 (19)

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  • 이 논문을 인용한 문헌 (1)

    1. Lee, Young-Guen 2007. "Elution Profiles of Volatile Compounds and Free Amino Acids during Alcohol Soaking of Garlic(Allum sativum L.)" 생명과학회지 = Journal of life science, 17(2): 286~292     

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