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Food science and biotechnology v.14 no.3 = no.63, 2005년, pp.350 - 354   피인용횟수: 8

Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cells by Essential Oil of Chrysanthemum boreale Makino

Cha, Jeong-Dan    (Major in Food and Nutrition, Wonkwang University   ); Jeong, Mi-Ran    (Institute of Oral Bioscience, Department of Oral Microbiology, School of Dentistry, Chonbuk National University   ); Lee, Young-Eun    (Professional Graduate School of Oriental Medicine, Wonkwang University  );
  • 초록

    The effect of the essential oil obtained from Chrysanthemum boreale Makino on the apoptosis of KB cells was investigated. Cytotoxicity and cellular DNA content were analyzed by MTT assay, flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. The various cytotoxic effects of the essential oil which are hallmarks of apoptosis, including DNA fragmentation, apoptotic body formation, and sub-G1 DNA content, all progressed in a dose-dependent manner. Treatment with an apoptosis-inducing concentration of the essential oil caused rapid and transient induction of caspase 3 activity. Further, the efficacious induction of PARP cleavage and caspase-3 activation was observed at an essential oil concentration of 0.1 and 0.2 mg/mL for 12 hr.

  • 주제어

    Chrysanthemum boreale Makino .   essential oil .   apoptosis .   caspase-3 .   PARP.  

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