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Food science and biotechnology v.14 no.3 = no.63, 2005년, pp.387 - 391   피인용횟수: 7

Rapid Enumeration of Listeria monocytogenes in Pork Meat Using Competitive PCR

Lim, Hyung-Kun    (Department of Biotechnology, Dongguk University   ); Hong, Chong-Hae    (Department of Veterinary Medicine, Kangwon National University   ); Choi, Weon-Sang    (Department of Biotechnology, Dongguk University  );
  • 초록

    Competitive polymerase chain reaction (cPCR) was used to develop a direct enumeration method of Listeria monocytogenes in pork meat. Pork meat was artificially inoculated with L. monocytogenes and DNA was extracted using guanidine thiocyanate-phenol-chloroform and subjected to PCR amplification. Sixteen primer sets for L. monocytogenes hlyA gene were tested for sensitive detection and the DG69/DG74 primer set was selected. The detection limit achieved with this primer set was as low as 860 colony-forming units (cfu) per 0.1 g of pork meat. When the samples were cultured at $30^{\circ}C$ for 16 hr in Brain Heart Infusion (BHI) medium, even a single bacterium could be detected with this primer set by PCR. For cPCR, the hlyA gene, which features a 148 bp-deletion, was cloned in the pGEM-4Z vector. A known amount of competitor DNA which has the same primer binding sites was co-amplified with L. monocytogenes total DNA from the artificially inoculated pork meat. The cell-number determined by cPCR was approximately equal to cfu from the Most Probable Number (MPN) method. The whole procedure took only 5 hr.


  • 주제어

    pork meat .   Listeria monocytogenes .   hlyA gene .   polymerase chain reaction .   competitive PCR (cPCR).  

  • 참고문헌 (22)

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  • 이 논문을 인용한 문헌 (7)

    1. 2006. "" Food science and biotechnology, 15(2): 324~327     
    2. 2006. "" Food science and biotechnology, 15(6): 967~974     
    3. Moon, Ae-Rie ; Choi, Weon-Sang 2007. "Rapid Enumeration of Salmonella spp. in Contaminated Pork Meat Using Competitive PCR" 한국식품위생안전성학회지 = Journal of food hygiene and safety, 22(4): 248~256     
    4. 2007. "" Food science and biotechnology, 16(4): 515~519     
    5. 2007. "" Food science and biotechnology, 16(3): 337~342     
    6. 2007. "" Food science and biotechnology, 16(3): 355~360     
    7. 2008. "" Food science and biotechnology, 17(4): 761~765     

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    10. 2001 "우유의 HACCP 시스템에서 Predictive Food Microbiology Model 이용" 한국식품위생안전성학회지 = Journal of food hygiene and safety 16 (2): 103~110    
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