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Food science and biotechnology v.14 no.3 = no.63, 2005년, pp.410 - 412   피인용횟수: 2

Analysis of Vibrio parahaemolyticus OMPs and Production of Antibodies against OMPs

Kim, Soo-Min    (Interdisciplinary Program in Bioinformatics, Seoul National University   ); Noh, Bong-Soo    (Dept. of Food and Microbial Technology, Seoul Women's University   ); Kim, Hae-Yeong    (Dept. of Food Biotechnology, Institute of Life Science, Kyung Hee University   ); Park, Se-Jin    (Dept. of Food Science and Nutrition & Research Institute of Human Ecology, Seoul National University   ); Ji, Geun-Eog    (Dept. of Food Science and Nutrition & Research Institute of Human Ecology, Seoul National University  );
  • 초록

    Vibrio parahaemolyticus is a gram-negative bacterium which acts as a causative agent for food poisoning. Studies with respect to specific extracellular proteins of V. parahaemolyticus would be useful for the development of specific detection methods against V. parahaemolyticus. In our present study, outer membrane proteins (OMPs) of V. parahaemolyticus were obtained from insoluble traction of 1% sarkosyl treated-cell wall materials. SDS-PAGE analysis showed the presence of several conserved outer membrane proteins among five strains of V. parahaemolyticus, and three bands were identified as V. parahaemolyticus OMPs through MALDI-TOF analysis. Polyclonal antibodies enriched with anti-OmpU were obtained from immunized rabbits. The antibodies against these proteins may be useful for the development of detection methods for V. parahaemolyticus.


  • 주제어

    Vibrio parahaemolyticus .   outer membrane proteins .   OmpU .   antibodies.  

  • 참고문헌 (16)

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  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" Food science and biotechnology, 15(4): 578~581     
    2. 2006. "" Food science and biotechnology, 15(6): 866~870     

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