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Food science and biotechnology v.14 no.6 = no.66, 2005년, pp.866 - 870   피인용횟수: 1
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The Binding of Aflatoxin $B_1$ Modulates the Adhesion Properties of Lactobacillus casei KCTC 3260 to a HT29 Colon Cancer Cell Line

Hwang, Kwon-Tack   (Biofood Research Center, Chonnam National UniversityUU0001112  ); Lee, Won-Jae   (Department of Food Science, University of Wisconsin-MadisonUU0015082  ); Kim, Gye-Yeop   (Department of Exercise Prescription, Dongshin UniversityUU0000476  ); Lee, Shin-Kyung   (Department of Food and Nutrition, Nambu UniversityUU0000326  ); Lee, Jeong-Min   (Department of Food and Nutrition, Nambu UniversityUU0000326  ); Jun, Woo-Jin   (Department of Food and Nutrition, Chonnam National UniversityUU0001112  );
  • 초록

    The 14 lactic acid bacteria (LAB) have been evaluated to determine the binding capacity to HT29 cell and Aflatoxin $B_1$ ( $AFB_1$ ). The interaction of LAB to HT29 cells has been further investigated to identify the possibility of competing the binding sites with $AFB_1$ . Of 14 LAB strains, Lactobacillus casei KCTC 3260 demonstrated the higher adhesiveness to HT29 and $AFB_1$ with the rate of 19.6% and 46.3%, respectively. In competitive analysis for binding sites, the adhesion of L. casei KCTC 3260 to HT29 cells was reduced with 100 nmol $AFB_1$ by 31.2%. The protoplast of L. casei KCTC 3260 showed no binding capacity to HT29 cells with increment of $AFB_1$ concentration, indicating that cell wall components might serve as a critical factor for the binding. To discriminate the major component influencing on L. casei KCTC 3260 binding to HT29 cells and $AFB_1$ , four different pre-treatments (lipase, pronase E, sodium m-periodate, and urea) were employed. Of those, sodium m-periodate treatment caused the lower adhesion of L. casei KCTC 3260 to HT29 cells with the increment of $AFB_1$ concentration. These results indicated that carbohydrate moiety on the cell wall of L. casei KCTC 3260 might be the most critical component in binding to both HT29 cells and $AFB_1$ .


  • 주제어

    lactobacillus .   HT29 cell .   aflatoxin $B_1$ .   cell wall .   adhesion.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2006. "" Food science and biotechnology, 15(6): 896~901     

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