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Immune network : official journal of the Korean association of immunobiologists v.5 no.4, 2005년, pp.199 - 204  
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Effect of Nitric Oxide on ADP-ribose Pyrophosphatase Activity

Kim, Jong-Hyun   (Department of Obstetrics & Gynecology, Chonbuk National University Medical SchoolUU0001120  );
  • 초록

    Background: ADP-ribosyl pyrophosphatases (ADPRase) has been known to catalyze the hydrolysis of ADP-ribose to ribose-5-phosphate and AMP. The role of ADPRase has been suggested to sanitize the cell by removing potentially toxic ADP-ribose. In this study, we examined the effect of nitric oxide on ADPRase activity in macrophages. Methods: ADPRase activity was measured in NO-inducing J774 cells. For in vitro experiments, recombinant human ADPRase was prepared in bacteria. Results: ADPRase activity was increased by the treatment of exogenous NO generating reagent, sodium nitroprusside (SNP), in J774 cells. The increased ADPRase activity was mediated by the post-translational modification, likely to cause cADP-ribosylation via nitrosylation of cysteine residue on the enzyme. The stimulation with endogeneous NO inducers, $TNF-{\alpha}/IFN-{\gamma}$ , also increased ADPRase activity through NO synthesis. Futhermore, ADPRase activity may be mediated by the post-translational modification of ADPRase, ADP-ribosylation. Conclusion: These results indicate that NO synthesized by macrophage activation plays a critical role in the increase in ADPRase activity following ADP-ribose metabolism.


  • 주제어

    Nitric oxide .   ADP-ribose pyrophosphatase .   macrophage .   ribosylation.  

  • 참고문헌 (18)

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 저자의 다른 논문

  • Kim, Jong-Hyun (2)

    1. 2003 "Polymerization of ADP-Ribose Pyrophosphatase: Conversion Mechanism of $Mg^{2+}-Dependent$ ADP-Ribose Pyrophosphatase into $Mg^{2+}-Independent$ Form" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 26 (10): 826~831    
    2. 2005 "대식세포의 Fc 수용체를 통한 탐식에 미치는 Inositol-phosphatase의 영향" Immune network : official journal of the Korean association of immunobiologists 5 (3): 144~149    

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