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Immune network : official journal of the Korean association of immunobiologists v.5 no.4, 2005년, pp.215 - 220  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

마우스 내장 림프조직에서 우세하게 발현되는 IgA Isotype Switching 관련 전사체의 분석
Preferential Expression of IgA Isotype Switching-associated Transcripts in Mouse Intestinal Lymphoid Tissues

채병철   (강원대학교 자연대학 미생물학과UU0000037  ); 전성기   (강원대학교 자연대학 미생물학과UU0000037  ); 서구영   (강원대학교 자연대학 미생물학과UU0000037  ); 김현아   (강원대학교 자연대학 미생물학과UU0000037  ); 김평현   (강원대학교 자연대학 미생물학과UU0000037  );
  • 초록

    Background: Transforming growth factor- ${\beta}$ (TGF- ${\beta}1$ ) directs class switch recombination (CSR) to IgA isotype, which is a predominant antibody in mucosal surfaces. Although IgA is preferentially committed in mucosal lymphoid tissues, it is not definitely established whether hallmarks of IgA CSR such as IgA germ-line transcripts (GLT ${\alpha}$ ), post-switch transcripts (PST ${\alpha}$ ) and circle transcripts (CT ${\alpha}$ ) are readily expressed in such tissues. Therefore, we compared the expression of these transcripts among mouse Peyer's patches (PP), mesenteric lymph nodes (MLN), and spleen. Methods: Levels of GLTs, PSTs and CTs were measured by RT-PCR in isolated PPs, MLNs and spleen cells. Results: GLT ${\alpha}$ and PST ${\alpha}$ were well expressed in PP and MLN cells but in spleen cells. Similar patterns were observed in the expression of GL ${\gamma}$ 2b and PST ${\gamma}$ 2b. On the other hand, these transcripts were only inducible in spleen cells upon stimulated with LPS and TGF- ${\beta}1$ . In addition, CT ${\alpha}$ and CT ${\gamma}$ 2b were detected in PP cells. Conclusion: PP B cells readily express IgA GLT, PST, and CT. Overall expression patterns of these transcripts were similar in MLN cells. Thus, these results suggest that microenvironment of PP and MLN influences spontaneous IgA CSR, which lacks in systemic lymphoid tissues such as spleen.


  • 주제어

    TGF- ${\beta}1$ .   IgA .   CSR .   GLT .   PST .   CT .   PPs .   MLNs.  

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