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콜라겐 유도 관절염에서 콜라겐 항원 특이 $V{\beta}3$+CD4+T 세포의 선택적 증식
Selective Expansion of TCR $V{\beta}3$+CD4+T Cells in Collagen-induced Arthritis in DBA/1 Mice

이재선   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 조미라   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 이정은   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 민소연   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 윤종현   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 김완욱   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 민준기   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 박성환   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  ); 김호연   (가톨릭대학교 의과학연구원 류마티스연구센터UU0000012  );
  • 초록

    Background: Collagen-induced arthritis (CIA) in mice is animal model of autoimmune disease known as rheumatic arthritis in human. We investigated CII-specific CD4+ T cell receptor usage in CIA mice. Methods: In CIA model, draining lymph node (dLN) CD4+ T cells and splenocytes at $3^{rd},\;5^{th},\;8^{th}$ week, we investigated CII-specific T cell proliferation, production of IL-17, IFN- ${\gamma}$ , TNF- ${\alpha}$ , IL-4 and IL-10. And we also performed anti-CII IgG Ab measurements in serum level, TCRV ${\beta}$ usage and T cell clonality with RT-PCR-SSCP analysis. Also, we performed proliferative response against CII when CII-specific T cell subset is deleted. Results: CIA mice showed more increase in the serum level of anti-CII IgG than normal mice after induction of arthritis. And the level of anti-CII IgG2a in CIA mice was increased after $3^{rd}$ week after primary immunization, while anti-CII IgG1 was decreased. Draining LN CD4+ T cells have proliferated against CII stimulation at $3^{rd}$ week after $1^{st}$ immunization. CD4+T cells derived from dLN of CIA mice produced proinflammatory cytokine IFN- ${\gamma}$ , IL-17 etc. Draining LN CD4 T cells of CIA presented higher proportion of CD4+V ${\beta}3$ +subset compared to those of normal mice at $3^{rd}$ week after $1^{st}$ immunization, and they were increased in proportion by CII stimulation. Draining LN CD4+ T cells without TCRV ${\beta}3+/V{\beta}8.1/8.2+/V{\beta}$ 10b+cells were not responsive against CII stimulation. But, CII-reactive response of TCRV ${\beta}3-/V{\beta}8.1/8.2-/V{\beta}$ 10b- T cells was recovered when $V{\beta}3+$ T cells were added in culture. Conclusion: Our results indicate that CD4+ $V{\beta}3+$ T cells are selectively expanded in dLN of CIA mice, and their recovery upon CII re-stimulation in vitro, as well as the production Th1-type cytokines, may play pivotal role in CIA pathogenesis.


  • 주제어

    TCRV ${\beta}3$ .   antigen-specific T cell .   type II collagen .   collagen-induced arthritis.  

  • 참고문헌 (20)

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