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The Expression of Galectin-3, a Beta-Galactoside Binding Protein, in Dendritic Cells

Kim, Mi-Hyoung   (Department of Veterinary Medicine, Cheju National UniversityUU0001165  ); Joo, Hong-Gu   (Department of Veterinary Medicine, Cheju National UniversityUU0001165  );
  • 초록

    Background: Dendritic cells (DCs) are the most potent APCs (antigen-presenting cells) and playa critical role in immune responses. Galectin-3 is a biological lectin with a beta-galactoside binding affinity. Recently, proteomic analysis revealed the presence of galectin-3 in the exosome of mature DCs. However, the expression and function of galectin-3 in DCs remains unclear yet. Methods: We used bone marrow-derived DCs of mouse and showed the expression of galectin-3 in DCs by using flow cytometry analysis and Western blot analysis. Results: Galectin-3 was determined as single band of 35 kDa in Western blot analysis. Flow cytometry analysis showed the major growth factor for DCs, granulocyte-macrophage colony stimulating factor (GM-CSF) and maturing agents, anti-CD40 monoclonal antibody (mAb) and lipopolysaccharide (LPS) consistently increased the intracellular expression of galectin-3 in DCs compared to medium alone. In addition, DCs treated with maturing agents did marginally express galectin-3 on their surface. Conclusion: This study suggests that galectin-3 in DCs may be regulated by critical factors for DC function.


  • 주제어

    Dendritic cells .   galectin-3 .   viability .   proliferaion.  

  • 참고문헌 (12)

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