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Purification and Characterization of ${\alpha}$-L-Arabinosidase from Trichoderma sp. SY

Jung, Bo-Ra   (Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk UniversityUU0000050  ); Kim, Bong-Gyu   (Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk UniversityUU0000050  ); Lee, Yoon-Jung   (Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk UniversityUU0000050  ); Ahn, Joong-Hoon   (Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk UniversityUU0000050  );
  • 초록

    Trichoderma sp. SY most effectively produces an extracellular ${\gamma}$ -L-arabinofuranosidase (AF) using arabinose as a carbon source. AF grown on cellulose as a carbon source was purified 28-fold with 4.4% yield by DEAE exchange and HQ/20 cation exchange chromatographies The purified enzyme was found to be homogeneous on SDS-PAGE with molecular weight of 89 kDa. It exhibited a high level of activity with p-nitrophenyl ${\alpha}$ -L-arabinofuranoside, showing $K_m$ and $V_{max}$ values of $0.15\;{\mu}M$ and $239.85U{\cdot}mg^{-1}$ , respectively and did not require any metal ion for activity. It also released p-nitrophenol from p-nitrophenol conjugated ${\beta}$ -D-xylopyranoside, and ${\beta}$ -D-galactopyranoside not from ${\beta}$ -D-glucopyranoside.


  • 주제어

    Trichoderma .   ${\alpha}$-L-arabinofuranoside .   arabinan.  

  • 참고문헌 (21)

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 저자의 다른 논문

  • Jung, Bo-Ra (1)

    1. 2004 "Purification and Characterization of $\beta$-Xylosidase from Trichoderma sp. SY" Journal of microbiology and biotechnology 14 (3): 643~645    
  • 김봉규 (19)

  • Lee, Yoon-Jung (3)

  • Ahn, Joong-Hoon (22)

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