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Agricultural chemistry & biotechnology v.48 no.1, 2005년, pp.16 - 25   피인용횟수: 1

Development of an ELISA for the Detection of Fenazaquin Residues in Fruits

Lee, Jae-Koo  
  • 초록

    To develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the residues of the acaricide fenazaquin, five haptens were synthesized and assessed. A competitive indirect format was used with polyclonal antibodies. Under an optimized condition using the selected rabbit C antiserum, an $IC_{50}$ of $96.97\;ng{\cdot}ml^{-1}$ , the detection range of $14.9{\sim}631\;ng{\cdot}ml^{-1}$ , and the lowest detection limit of $8\;ng{\cdot}ml^{-1}$ were obtained. Some structurally related compounds of practical use showed low crossreactivities to the antibody. Highest cross-reactivity observed with hapten IV indicates that the antiserum C recognizes very well quinazoline ring, 4-tert-butylphenyl, and an adequate length of spacer arm. The length of spacer arm affected recognition of quinazoline ring and 4-tert-butylphenyl moieties. When applied to apple and pear, recoveries were within acceptable ranges of $93.18{\sim}104.77%$ (n = 4) and $79.40{\sim}111.95%$ (n = 4), respectively.


  • 주제어

    ELISA .   polyclonal antibody .   fenazaquin .   cross-reactivity .   monitoring.  

  • 참고문헌 (22)

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    16. An immunoassay for pyrethroids: Detection of permethrin in meat , Stanker, L.H.;Bigbee, C.;Van Emon, J.;Watkins, B.;Jensen, R.H.;Morris, C.;Vanderlaan, M. , J. Agric. Food Chem. / v.37,pp.834-839,
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    22. Development of an enzyme-linked immunosorbent assay for the detection of the fungicide fenarimol , Lee, J.K.;Park, S.H.;Lee, E.Y.;Kim, Y.J.;Kyung, K.S. , J. Agric. Food Chem. / v.52,pp.7206-7213,
  • 이 논문을 인용한 문헌 (1)

    1. 2009. "" Journal of the Korean Society for Applied Biological Chemistry, 52(4): 380~388     

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