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Asian-Australasian journal of animal sciences v.18 no.1, 2005년, pp.17 - 21   피인용횟수: 4

Effects of Melatonin on Gene Expression of IVM/IVF Porcine Embryos

Jang, H.Y.    (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University   ); Kong, H.S.    (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University   ); Choi, K.D.    (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University   ); Jeon, G.J.    (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University   ); Yang, B.K.    (College of Animal Resources Science, Kangwon National University   ); Lee, C.K.    (School of Agricultural Biotechnology, Seoul National University   ); Lee, H.K.    (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University  );
  • 초록

    The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR. Porcine embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% $CO_2$ and 20% $O_2$ at $37^{\circ}C$ in NCSU23 medium. Melatonin was added to medium at concentration of 1nM, 5 nM, and 10 nM. When treated with 1nM (39.0%) of melatonin, the developmental rate of embryos beyond the morula stage were higher than that of control group (31.0%) (p ${\pm}$ 0.5 and 17.3 ${\pm}$ 0.8), 1 nM (23.6 ${\pm}$ 0.6 and 19.0 ${\pm}$ 0.5), and 5 nM (23.3 ${\pm}$ 1.1 and 16.3 ${\pm}$ 0.8) treated with melatonin were higher than in 10 nM (20.0 ${\pm}$ 0.5 and 13.3 ${\pm}$ 0.8) treated with melatonin (p


  • 주제어

    Porcine Embryo .   RT-PCR .   Catalase .   Bax .   Caspase-3 .   Melatonin.  

  • 참고문헌 (21)

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  • 이 논문을 인용한 문헌 (4)

    1. 2007. "" Asian-Australasian journal of animal sciences, 20(12): 1820~1826     
    2. 2007. "" Asian-Australasian journal of animal sciences, 20(10): 1510~1516     
    3. 2007. "" Asian-Australasian journal of animal sciences, 20(5): 693~700     
    4. 2008. "" Asian-Australasian journal of animal sciences, 21(7): 980~987     

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