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Asian-Australasian journal of animal sciences v.18 no.4, 2005년, pp.512 - 515   피인용횟수: 4

Effect of C18-polyunsaturated Fatty Acids on Their Direct Incorporation into the Rumen Bacterial Lipids and CLA Production In vitro

Choi, S.H.    (Department of Animal Science, Chungbuk National University   ); Song, M.K.    (Department of Animal Science, Chungbuk National University  );
  • 초록

    An in vitro study was conducted to determine the effect of C18-polyunsaturated fatty acid on direct incorporation into the rumen bacteria, bio-hydrogenation and production of CLA in vitro. Sixty milligrams of linoleic acid ( $C_{18:2}$ ) or linolenic acid ( $C_{18:3}$ ) were absorbed into the 0.5 g cellulose powder was added to the 150 ml culture solution consisting of 120 ml McDougall's buffer and 30 ml strained rumen fluid. Four uCi of 1- $^{14}C_{18:2}$ or 1- $^{14}C_{18:3}$ (1 uCi/15 mg each fatty acid) were also added to the corresponding fatty acids to estimate the direct incorporation into the bacterial lipids. The culture solution was then incubated anaerobically in a culture jar with stirrer at 39 $^{\circ}C$ for 12 h. Ammonia concentration and pH of the culture solution were slightly influenced by the fatty acids. Amount of fatty acid incorporated into the bacteria was 1.20 mg and 0.43 mg/30 ml rumen fluid for $C_{18:2}$ and $C_{18:3}$ , respectively during 12 h incubation. Slightly increased CLA (sum of cis-9, trans-11 and cis-10, trans-12 $C_{18:2}$ ) was obtained from the $C_{18:3}$ addition compared to that from $C_{18:2}$ after 12 h incubation in vitro.


  • 주제어

    Linoleic Acid .   Linolenic Acid .   Bio-hydrogenation .   CLA .   Rumen Bacteria.  

  • 참고문헌 (15)

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    6. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseed. Asian-Aust. J. Anim. Sci. 15:687-694. 
    7. Knight, R., J. D. Sutton, J. E. Storry and P. E. Brumby. 1979. Rumen microbial synthesis of long-chain fatty acids. Proc. Nutr. Soc. 38:4A. 
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    10. McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheeps saliva. Biochem. J. 43:99-109. 
    11. Wang, J. H., S. H. Choi and M. K. Song. 2003. Effects of concentrate to roughage ratio on the formation of cis-9, trans-11 CLA and trans-11 octadecenoic acid in rumen fluid and plasma of sheep when fed high oleic or high linoleic acid oils. Asian-Aust. J. Anim. Sci. 16:1604-1609. 
    12. Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225. 
    13. Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipid in a one-step reaction. J. Lipid Research. 27:114-121. 
    14. Harfoot, C. G. and G. P. Hazlewood. 1988. Lipid metabolism in the rumen. p. 285-322 in The rumen microbial ecosystem (Ed. P. N. Hobson) Elsevier Appl. Sci. London and New York. 
    15. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002b. Addition effect of seed-associated or free linseed oil on the formation of cis-9, trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro. Asian-Aust. J. Anim. Sci. 15:1115-1120. 
  • 이 논문을 인용한 문헌 (4)

    1. 2007. "" Asian-Australasian journal of animal sciences, 20(10): 1525~1538     
    2. 2007. "" Asian-Australasian journal of animal sciences, 20(9): 1417~1423     
    3. 2007. "" Asian-Australasian journal of animal sciences, 20(11): 1694~1698     
    4. 2009. "" Asian-Australasian journal of animal sciences, 22(6): 819~826     

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