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Polymorphism of the Promoter Region of Hsp70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in Lymphocytes in Peripheral Blood of Heat Shocked Dairy Cows

Cai, Yafei    (College of Animal Science and Technology, Nanjing Agricultural University   ); Liu, Qinghua    (College of Animal Science and Technology, Nanjing Agricultural University   ); Xing, Guangdong    (College of Animal Science and Technology, Nanjing Agricultural University   ); Zhou, Lei    (College of Animal Science and Technology, Nanjing Agricultural University   ); Yang, Yuanyuan    (College of Animal Science and Technology, Nanjing Agricultural University   ); Zhang, Lijun    (College of Animal Science and Technology, Nanjing Agricultural University   ); Li, Jing    (Center for Molecular Recognition, Columbia University   ); Wang, Genlin    (College of Animal Science and Technology, Nanjing Agricultural University  );
  • 초록

    The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax- $\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax- $\alpha$ . The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax- $\alpha$ mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.


  • 주제어

    Polymorphism .   hsp70 Gene .   Dairy Cow .   HSP70mRNA .   HSF1mRNA .   Bcl-2mRNA/Bax- $\alpha$mRNA.  

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