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뇌하수체 제거술이 수행된 랫트의 혈액학 및 혈액화학적 소견
Hematological and blood chemical findings in hypophysectomized rats

김남중    (혜천대학 애완동물자원과   ); 김명철    (충남대학교 수의과대학  );
  • 초록

    The present study was carried out to produce the hematological and blood chemical findings after hypophysectomy in rats. Hypophysectomy was performed by the parapharyngeal method and the sham surgery was performed for the control group. Two weeks after the operation, the body weight of the hypophysectomized and control rats was measured daily for 5 days. We deleted the rats the weight gain of which is less than 5 g during 5 days from the hypophysectomy group. The successful operation rate was approximately 40%. In the hypophysectomized and control rats, their blood samples were collected from posterior vena cava after celiotomy under generally anesthesia with ether. Hematological parameters such as erythrocyte count, leukocyte count, hemoglobin concentration, hematocrit level, and platelet count were determined by Animal Blood Counter. The erythrocyte count, hemoglobin concentration, and hematocrit level were lower significantly (p 0.05) between hypophysectomy group and control group. Also, blood chemical parameters such as glucose, blood urea nitrogen (BUN), aspartate animotransferase, albumin, total protein, cholesterol, calcium, and magnesium in serum were determined. Except BUN concentration, all parameters were not affected by hypophysectomy. But the BUN concentration was higher significantly (p


  • 주제어

    hypophysectomy .   hematological findings .   rat.  

  • 참고문헌 (18)

    1. Bauman DE. Bovine somatotropin: review of an emerging animal technology. J Dairy Sci 1992, 75, 3432-3451 
    2. Meji BP, Voorhout G, Van Den Ingh TS, Hazewinkel HA, Teske E, Rijnberk A. Results of transsphenoidal hypophysectomy in 52 dogs with pituitary-dependent hyperadrenocorticism. Vet Surg 1998, 27, 246-261 
    3. Niebauer GW, Evans SM. Transsphenoidal hypophysectomy in the dog. A new technique. Vet Surg 1988, 17, 296-303 
    4. Engstrom G, Lindstrom P, Savendahl L. Lack of evidence for acute effects of growth hormone-releasing hormone on serum insulin and glucose levels normal and hypophysectomized rats. Horm Res 1994, 41, 21-26 
    5. Groesbeck MD, Parlow AF. Highly improved precision of the hypophysectomized female rat body weight gain bioassay for growth hormone by increased frequency of injections, avoidance of antibody formation, and other simple modifications. ENDO 1987, 120, 2582-2590 
    6. Naik DV, Sheriff AQ. An improved technique for transauricular hypophysectomy and auto transplant of isolated pieces of pars intermedia tissue in the evacuated pituitary capsule of the rat. Acta Endocrinol 1975, 79, 677-687 
    7. Shaar CJ, Tinsley FC, Smith MC, Clemens JA, Neubauer BL. Recombinant DNA-derived human insulin-like growth factor II (hIGF-II) stimulates growth in hypophysectomized rats. Endocr Res 1989, 15, 403-411 
    8. Park SJ, Kim NJ, Kwon SC, Lee SJ, Cho JM. Efficacy tests of recombinant human growth hormone produced from saccharomyces cerevisiae. J Biochem Mol Biol 1995, 28, 437-442 
    9. Meji BP, Voorhout G, Van Den Ingh TS, Rijnberk A. Transsphenoidal hypophysectomy for treatment of pituitary-dependent hyperadrenocorticism in 7 cats. Vet Surg 2001, 30, 72-86 
    10. Asakawa K, Hizuka N, Takano K, Horikawa R, Sukegawa I, Toyoda C, Shizume K. Human growth hormone stimulates liver regeneration in rats. J Endocrinol Invest 1989, 12, 343-347 
    11. Mol JA, Rijnberk A. Pituitary function. In: Kaneko J. Harvey J, Bruss M(eds.). Clinical Biochemistry of Domestic Animals. pp. 576-609, 4th ed. Academic Press, Washington DC, 1989 
    12. Meji BP, Voorhout G, Van Den Ingh TS, Hazewinkel HA, Van't Verlaat JW. Transsphenoidal hypophysectomy in beagle dogs: evaluation of a microsurgical technique. Vet Surg 1997, 26, 295-309 
    13. Gregory FG. A Technique for hypophysectomy of neonatal rats. Life Sci 1980, 26, 971-977 
    14. Engstrom KG, Ohlsson L, Oscarsson J. Effect of hypophysectomy and growth hormone substitution on red blood cell morphology and filterability in rats. J Lab Clin Med 1990, 116, 196-205 
    15. Niebauer GW, Eigenmann JE, Van Winkle TJ. Study of long-term survival after trassphenoidal hypophysectomy in clinically normal dogs. Am J Vet Res 1990, 51, 677-681 
    16. Waynforth HB. Hypophysectomy. In: Waynforth HB, Flecknell PA (eds.). Experimental and Surgical Technique in the Rat. pp. 143-150, 1st ed. Academic Press, London, 1980 
    17. Schwartz A. Endocrine System. In: Textbook of Small Animal Surgery. pp. 1840-1851, 1st ed. Saunders, San Diego, 1985 
    18. Meij BP. Hypophysectomy as a treatment for canine and feline Cushing's disease. Vet Clin North Am Small Anim Pract 2001, 31, 1015-1041 

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